目的探索成年大鼠皮下微环境对移植的幼年SD大鼠睾丸中睾丸间质细胞存活、发育及雄激素分泌的影响。方法将成年雄性SD大鼠随机分为对照组、假手术组、去势组和未去势组。对照组大鼠不进行任何处理;假手术组大鼠去势后不进行睾丸移植;去...目的探索成年大鼠皮下微环境对移植的幼年SD大鼠睾丸中睾丸间质细胞存活、发育及雄激素分泌的影响。方法将成年雄性SD大鼠随机分为对照组、假手术组、去势组和未去势组。对照组大鼠不进行任何处理;假手术组大鼠去势后不进行睾丸移植;去势组大鼠于去势1周后行背部双侧睾丸移植手术,每侧移植1个5~7 d SD大鼠睾丸组织;未去势组直接在正常大鼠背部两侧各移植1个幼年SD大鼠睾丸组织。移植后第4周取材、称量移植睾丸组织质量,HE染色分析移植睾丸组织的组织学特点,免疫组织化学染色法检测移植睾丸组织中17β-羟基类固醇脱氢酶1(HSD-17β1)表达和分布,同时采用ELISA检测受体血清睾酮水平。结果去势组移植睾丸组织质量明显大于未去势组。去势组和未去势组均可见睾丸间质细胞,免疫组织化学染色发现去势组移植睾丸组织中HSD-17β1阳性细胞较未去势组多。ELISA分析发现对照组和未去势组的血清雄激素水平均高于假手术组和去势组,且去势组明显高于假手术组。结论移植的幼年大鼠睾丸组织可在成年受体大鼠皮下进一步发育,形成的睾丸间质细胞还具备一定的雄激素合成和分泌功能。展开更多
Rat testis transplantation with a segment of donor abdominal aorta end-to-side anastomosed to recipient’s abdominal aorta was first reported by Lee.~1 But thrombus was formed in the donor abdominal aorta segment. Gol...Rat testis transplantation with a segment of donor abdominal aorta end-to-side anastomosed to recipient’s abdominal aorta was first reported by Lee.~1 But thrombus was formed in the donor abdominal aorta segment. Goldsein^2 and Lee^3 (improved this) technique using end-to-side anastomosis of an aortic patch with the testicular artery to the recipient’s aorta and the testicular vein was anastomosed to the vena cava of the recipient. With this method, the animal with the testicular artery and vein paralleling and originating no more than 4 mm apart is considered a suitable donor, which is scarce because of the variability of testicular vessels. This problem limits the wide use of testicular transplantation in rats. Based on these findings, we established a novel testicular transplantation model which was recreated as closely as possible to the normal vascular anatomy of the testis in rats. The functional activities of these transplants were also presented.展开更多
文摘目的探索成年大鼠皮下微环境对移植的幼年SD大鼠睾丸中睾丸间质细胞存活、发育及雄激素分泌的影响。方法将成年雄性SD大鼠随机分为对照组、假手术组、去势组和未去势组。对照组大鼠不进行任何处理;假手术组大鼠去势后不进行睾丸移植;去势组大鼠于去势1周后行背部双侧睾丸移植手术,每侧移植1个5~7 d SD大鼠睾丸组织;未去势组直接在正常大鼠背部两侧各移植1个幼年SD大鼠睾丸组织。移植后第4周取材、称量移植睾丸组织质量,HE染色分析移植睾丸组织的组织学特点,免疫组织化学染色法检测移植睾丸组织中17β-羟基类固醇脱氢酶1(HSD-17β1)表达和分布,同时采用ELISA检测受体血清睾酮水平。结果去势组移植睾丸组织质量明显大于未去势组。去势组和未去势组均可见睾丸间质细胞,免疫组织化学染色发现去势组移植睾丸组织中HSD-17β1阳性细胞较未去势组多。ELISA分析发现对照组和未去势组的血清雄激素水平均高于假手术组和去势组,且去势组明显高于假手术组。结论移植的幼年大鼠睾丸组织可在成年受体大鼠皮下进一步发育,形成的睾丸间质细胞还具备一定的雄激素合成和分泌功能。
文摘Rat testis transplantation with a segment of donor abdominal aorta end-to-side anastomosed to recipient’s abdominal aorta was first reported by Lee.~1 But thrombus was formed in the donor abdominal aorta segment. Goldsein^2 and Lee^3 (improved this) technique using end-to-side anastomosis of an aortic patch with the testicular artery to the recipient’s aorta and the testicular vein was anastomosed to the vena cava of the recipient. With this method, the animal with the testicular artery and vein paralleling and originating no more than 4 mm apart is considered a suitable donor, which is scarce because of the variability of testicular vessels. This problem limits the wide use of testicular transplantation in rats. Based on these findings, we established a novel testicular transplantation model which was recreated as closely as possible to the normal vascular anatomy of the testis in rats. The functional activities of these transplants were also presented.
