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The effects of DDT on the feeding, respiration, survival, and reproduction of Sinocalanus tenellus(Copepoda:Calanoida) 被引量:3
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作者 XU Donghui LIU Guangxing 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2014年第9期133-138,共6页
DDT (dichloro-diphenyl-trichloroethane) as a type of organochlorine pesticides, is an important compo nent of pesticides pollution whose impact on the marine ecosystem is urgently to be evaluated. To investi gate th... DDT (dichloro-diphenyl-trichloroethane) as a type of organochlorine pesticides, is an important compo nent of pesticides pollution whose impact on the marine ecosystem is urgently to be evaluated. To investi gate the biological effects of DDT on the marine ecosystem, copepods being the main contributor of sec ondary productivity in the marine ecosystem, were selected as target animals. The influence of DDT on the feeding, respiration, survival, and reproduction of Sinocalanus tenellus (S. tenellus) was analyzed and the antioxidant enzymes activities in the individuals were measured under different exposure concentrations of DDT. The 48 h median lethal concentration (LC50) and 96 h LC50 of DDT to S. tenellus were 5.44 and 2.50 pg/dma, respectively. The filtration rates, grazing rates, and respiration of S. tenellus decreased apparently with increased DDT concentrations. Under lower concentration (〈625 ng/dm3) of DDT, the activities of the antioxidant enzymes, including superoxide dismutase and catalase in the animals increased significantly compared with those in the animals without any exposure to DDT, which suggested that the antioxidant enzymes can protect the animals from oxidative damage. However, the activity of the antioxidant enzyme decreased when the animals were exposed to higher concentration (1250-2 500 ng/dma) of DDT. The sur-vival rate of both females and males was reduced when they were exposed to DDT less than 250 ng/dm3, but females showed higher survival rate than males when they are under the same concentration. The hatching ratio and the egg diameters of S. tenellus decreased significantly when they were exposed to DDT with a concentration of 25 and 250 ng/dm3, however, the cumulative egg production did not show any significant variation when the animals were exposed to the above DDT concentration. These data in the preset study suggested that exposure to DDT can cause the variation of the species composition of copepods, and further affect the marine ecosystem. 展开更多
关键词 DDT Sinocalanus tenellus SURVIVAL REPRODUCTION FEEDING RESPIRATION antioxidant enzyme
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Ethanol extract of Chondracanthus tenellus(Harvey)Hommersand attenuates lipopolysaccharide-induced inflammatory and oxidative response by blocking the NF-κB,MAPKs,and PI3K/Akt signaling pathways 被引量:6
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作者 Da Hye Kwon Cheol Park +7 位作者 Hyesook Lee Su Hyun Hong Gi-Young Kim Hee-Jae Cha Suhkmann Kim Heui-Soo Kim Hye-Jin Hwang Yung Hyun Choi 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第10期450-459,共10页
Objective:To investigate whether the ethanol extract of Chondracanthus tenellus(Harvey)Hommersand,a type of red algae,could exhibit anti-inflammatory potential in lipopolysaccharide(LPS)-stimulated macrophages.Methods... Objective:To investigate whether the ethanol extract of Chondracanthus tenellus(Harvey)Hommersand,a type of red algae,could exhibit anti-inflammatory potential in lipopolysaccharide(LPS)-stimulated macrophages.Methods:The ethanol extract of Chondracanthus tenellus was applied to 100 ng/mL LPS-stimulated RAW 264.7 cells,and cell viability,phagocytic ability,levels of pro-inflammatory factors,and the production of reactive oxygen species were measured.To identify the underlying mechanism of the ethanol extract of Chondracanthus tenellus,the expression of inflammation-regulated genes was estimated.Results:The ethanol extract of Chondracanthus tenellus had no cytotoxic effect at concentrations below 300μg/mL,and reduced the LPS-induced production of inflammatory mediators including nitric oxide(NO)and prostaglandin E2.Furthermore,the extract markedly suppressed the expression of inducible NO synthase and cyclooxygenase-2,as well as the production of reactive oxygen species.The LPS-induced up-regulation of pro-inflammatory cytokines was attenuated by treatment with the ethanol extract of Chondracanthus tenellus,reducing their extracellular secretion.The Chondracanthus tenellus extract also inhibited LPS-mediated activation of nuclear factor-kappa B(NF-κB).In addition,the phosphorylation of mitogen activated protein kinases(MAPKs)and phosphatidylinositol 3 kinase(PI3K)/Akt was markedly increased by LPS,which was significantly abolished by the Chondracanthus tenellus extract.Conclusions:Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-κB,MAPKs,and PI3K/Akt signaling pathways in LPS stimulated RAW 264.7 macrophages.tenellus extract.Conclusions:Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-κB,MAPKs,and PI3K/Akt signaling pathways in LPS stimulated RAW 264.7 macrophages. 展开更多
关键词 Chondracanthus tenellus Inflammation ROS NF-ΚB RAW 264.7 cells
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Immunostimulatory effect of ethanol extract of Chondracanthus tenellus in RAW 264.7 macrophages in vitro 被引量:1
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作者 Cheol Park Da Hye Kwon +8 位作者 Hyesook Lee Su Hyun Hong Gi-Young Kim Hee-Jae Cha Do-Hyung Kim Suhkmann Kim Heui-Soo Kim Hye-Jin Hwang Yung Hyun Choi 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2021年第6期263-272,共10页
Objective:To investigate whether ethanol extracts of Chondracanthus tenellus(EECT)could improve immunomodulatory property of murine monocyte/macrophage RAW 264.7 cells.Methods:Cell viability,phagocytic ability,and nit... Objective:To investigate whether ethanol extracts of Chondracanthus tenellus(EECT)could improve immunomodulatory property of murine monocyte/macrophage RAW 264.7 cells.Methods:Cell viability,phagocytic ability,and nitric oxide were measured.The levels of prostaglandin E2 and cytokines were determined using enzyme-linked immunosorbent assay kits.Expression of immunoregulatory response protein was detected by Western blotting assay.Results:As the concentration of EECT increased,the morphology of the cells changed to a typical active macrophage shape,and the phagocytic activity increased significantly.EECT also effectively enhanced the production and secretion of immunomodulatory mediators,such as nitric oxide and prostaglandin E2,and cytokines.In addition,compared with the control group,EECT markedly stimulated the expression of Toll-like receptor 4(TLR4)and myeloid differentiation factor 88,one of the TLR4 adapter molecules.Furthermore,EECT promoted the nucleus translocation of nuclear factor-kappa B(NF-κB)by increasing the phosphorylation and degradation of the inhibitor of NF-κB-α,indicating activation of the NF-κB signaling pathway.Meanwhile,similar trends were found in cells treated with lipopolysaccharide as a positive control.Conclusions:Taken together,the results indicate that EECT has an immunomodulatory effect by increasing the production of immunomodulatory mediators and cytokines through activation of the TLR4/NF-κB signaling pathway.EECT could be used as a potential candidate for medication or dietary supplements to increase immune activity. 展开更多
关键词 Chondracanthus tenellus Immunomodulatory property TLR4 NF-κB
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几个试验因子对细巧华哲水蚤清滤率和滤食率的影响 被引量:12
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作者 陆开宏 吕耀平 +2 位作者 林霞 郑祯 姚国琴 《海洋科学》 CAS CSCD 北大核心 2001年第4期44-47,共4页
以象山港浮游桡足类优势种细巧华哲水蚤 (Sinocalanustenellus)成体为试验动物投喂以大溪地等鞭金藻(Isochrysisgalbona),对其清滤率F(ml/个·h)和滤食率G(细胞/个·h)进行了实验测定。结果表明 ,温度、海水比重、食物浓度及试... 以象山港浮游桡足类优势种细巧华哲水蚤 (Sinocalanustenellus)成体为试验动物投喂以大溪地等鞭金藻(Isochrysisgalbona),对其清滤率F(ml/个·h)和滤食率G(细胞/个·h)进行了实验测定。结果表明 ,温度、海水比重、食物浓度及试验动物密度的变化对细巧华哲水蚤清滤率和滤食率均有显著影响。水温15℃、比重1.010、食物浓度150000个细胞/ml和试验动物密度0.25个/ml的条件下 ,细巧华哲水蚤滤食率达到最大。 展开更多
关键词 细巧华哲水蚤 清滤率 滤食率 影响因子 饲料
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盐碱池塘细巧华哲水蚤对浮游植物的摄食生态研究 被引量:9
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作者 赵文 董双林 《生态学报》 CAS CSCD 北大核心 2002年第5期682-687,共6页
利用常规显微镜直接计数法和叶绿素 a法研究了盐碱池塘细巧华哲水蚤 ( Sinocalanustenellus)对浮游藻类的原位摄食率及其影响因素。测得该水蚤对水柱浮游植物的滤水率为 1 .73~ 1 5 5 .1 6μl/ ( ind.· h) ,摄食率为 92~ 2 5 38c... 利用常规显微镜直接计数法和叶绿素 a法研究了盐碱池塘细巧华哲水蚤 ( Sinocalanustenellus)对浮游藻类的原位摄食率及其影响因素。测得该水蚤对水柱浮游植物的滤水率为 1 .73~ 1 5 5 .1 6μl/ ( ind.· h) ,摄食率为 92~ 2 5 38cell/( ind.· h)或 0 .7~ 2 7.7ng C/ ( ind.· h)。两种方法测定的滤水率和摄食率基本相符 ( p>0 .5 ,t检验 )。该水蚤可有选择地滤食诸如小色球藻、光甲藻、绿裸藻 ,单生卵囊藻和小环藻等 ,其选择性指数分别为 0 .81、0 .75、0 .72、0 .6 1和 0 .35。不同光处理下细巧华哲水蚤的滤食率多数情况是无光时摄食率高。在本实验的条件下该水蚤在饥饿后的滤水率和摄食率与正常情况相比均显著降低 ( P<0 .0 1 ,t检验 )。 展开更多
关键词 盐碱池塘 细巧华哲水蚤 浮游植物 摄食率 生态系统
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生态育苗池中的桡足类与河蟹苗产量的关系 被引量:8
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作者 张清靖 李晓东 +2 位作者 朱华 刘胥 李永函 《应用生态学报》 CAS CSCD 北大核心 2007年第1期174-178,共5页
探讨了辽宁盘锦辽河三角洲地区中华绒螯蟹(Eriocheir sinensis)生态育苗池中出现的近亲真宽水蚤(Eurytemor affinis)和细巧华哲水蚤(Sinocalanus tenellus)与中华绒螯蟹幼体的关系.结果表明:近亲真宽水蚤和细巧华哲水蚤都严重地影响Ⅰ... 探讨了辽宁盘锦辽河三角洲地区中华绒螯蟹(Eriocheir sinensis)生态育苗池中出现的近亲真宽水蚤(Eurytemor affinis)和细巧华哲水蚤(Sinocalanus tenellus)与中华绒螯蟹幼体的关系.结果表明:近亲真宽水蚤和细巧华哲水蚤都严重地影响Ⅰ期中华绒螯蟹溞状幼体的成活率,其密度越大,Ⅰ期溞状幼体的成活率就越低(P<0.01);Ⅲ期中华绒螯蟹溞状幼体可捕食桡足类无节幼体,且捕食量随着无节幼体密度增加而变大(P<0.01);Ⅴ期中华绒螯蟹溞状幼体和大眼幼体容易捕食到近亲真宽水蚤而很难捕食到细巧华哲水蚤;蟹苗池中大眼幼体的产量与育苗初期池塘中桡足类的数量呈负相关关系,且不同桡足类密度下大眼幼体收获量差异极显著(P<0.01).提出了控制和利用蟹苗池中桡足类的措施. 展开更多
关键词 近亲真宽水蚤 细巧华哲水蚤 中华绒螯蟹幼体 生态育苗池
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温度和盐度对细巧华哲水蚤存活率的影响 被引量:5
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作者 林霞 李春月 陆开宏 《宁波大学学报(理工版)》 CAS 2001年第1期43-46,共4页
研究了温度、盐度及其交互作用对细巧华哲水蚤 24 h存活率的影响.结果表明:该种适温范围1~27℃,最适温度为15~20℃;适盐范围0~29.3‰,最适盐度为12.4~18.9.方差分析显示了温度、盐度及其交互作用对存... 研究了温度、盐度及其交互作用对细巧华哲水蚤 24 h存活率的影响.结果表明:该种适温范围1~27℃,最适温度为15~20℃;适盐范围0~29.3‰,最适盐度为12.4~18.9.方差分析显示了温度、盐度及其交互作用对存活率均有极显著影响,依贡献大小为温度>盐度>交互作用.当培养条件接近自然海区的温度或盐度时,细巧华哲水蚤具有最大的适盐、适温范围. 展开更多
关键词 细巧华哲水蚤 存活率 温度 盐度 适盐范围 适温范围 桡足类
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中国山西、广西美芒藻属两新种 被引量:4
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作者 谢树莲 凌元洁 《植物分类学报》 CSCD 1998年第1期81-83,共3页
中国山西、广西美芒藻属两新种谢树莲凌元洁(山西大学生命科学系太原030006)TWONEWSPECIESOFCOMPSOPOGON(RHODOPHYTA)FROMSHANXIANDGUANGXI,CHINAXIES... 中国山西、广西美芒藻属两新种谢树莲凌元洁(山西大学生命科学系太原030006)TWONEWSPECIESOFCOMPSOPOGON(RHODOPHYTA)FROMSHANXIANDGUANGXI,CHINAXIEShuLianLINGYuanJ... 展开更多
关键词 美芒藻属 细弱美芒藻 疏枝美芒藻 山西 广西 中国
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湖南益阳下奥陶统的细弱匿笔石Adelograptus tenellus(Linnarsson,1871) 被引量:5
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作者 王文卉 冯洪真 +2 位作者 李丽霞 李明 陈文建 《古生物学报》 CAS CSCD 北大核心 2012年第2期186-199,共14页
湖南省益阳市南坝地区下奥陶统印渚埠组产出大量保存良好的细弱匿笔石Adelograptus tenellus(Linnarsson,1871)。在对该种进行详细的系统古生物学研究后,选取重要性状讨论该区A.tenellus的居群内形态变异。在此基础上,综合考虑A.tenellu... 湖南省益阳市南坝地区下奥陶统印渚埠组产出大量保存良好的细弱匿笔石Adelograptus tenellus(Linnarsson,1871)。在对该种进行详细的系统古生物学研究后,选取重要性状讨论该区A.tenellus的居群内形态变异。在此基础上,综合考虑A.tenellus各级枝的长度、胞管组合以及胞管密度等重要性状,将益阳地区的A.tenellus标本分为两种形态类型(类型Ⅰ和Ⅱ),并结合二者的地层分布,以及与北欧、英国、北美等地的对比,提出形态类型Ⅰ向形态类型Ⅱ的演替过程。认为英国、新西兰、皖南等地的A.tenellus与当前形态类型Ⅰ相似;北欧地区的A.tenellus则与当前形态类型Ⅱ较为相似。 展开更多
关键词 Adelograptus tenellus(Linnarsson 1871) 形态变异 下奥陶统 湖南益阳
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