Background Catestatin, a chromogranin A-derived peptide, is a potent antagonist of nicotine-evoked catecholamine release. We know that catecholamine plays an important role in cardiovascular remodeling induced by hype...Background Catestatin, a chromogranin A-derived peptide, is a potent antagonist of nicotine-evoked catecholamine release. We know that catecholamine plays an important role in cardiovascular remodeling induced by hypertension, therefore we hypothesized that catestatin would affect target-organ structure during hypertension. Methods Twelve spontaneously hypertensive rats （SHRs） were randomized to SHR control group and catestatin group, the normal control group was comprised of six healthy Wistar-Kyoto rats of the same age. Tail-cuff blood pressure and pulse rate were obtained at weeks 1, 4 and 8. At the end of the eight-week period, the heart, abdominal aorta and left kidney were excised and weighed, VG staining was done and the intima-media thickness of vessels and the collagen volume fraction were assessed by an image acquisition and analysis system. The proliferating cell nuclear antigen （PCNA） was observed by immunohistochemistry, and real time reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the mRNA levels of proliferative genes including cyclin A, ki67 and PCNA in the abdominal aorta. Results All the parameters in SHR observed in the present study increased significantly compared to Wistar Kyoto rats （P 〈0.01 ）. With intervention with catestatin, the systolic blood pressure decreased slightly but it was not significantly different from the SHR control, the cardiac mass index and left ventricular mass index both decreased significantly, the collagen volume fraction decreased by nearly 30% in the heart, by 25% in vessels and by 10% in the kidney, and the intima-media thickness and expression of proliferative genes, including cyclin A, ki67 and PCNA, in the abdominal aorta also decreased significantly. Conclusions The present study indicated that catestatin could ameliorate proliferating changes of heart, kidney and vessels during hypertension, especially to the deposition of interstitial collagen. Blood pressure was not the main factor to mediate this effect, which展开更多
目的探讨老年原发性高血压患者血压变异性(BPV)与靶器官损害的关系。方法①收集符合纳入标准的老年人群资料,分为高血压组(n=146)和正常对照组(n=51),比较两组24 h收缩压/舒张压变异性(24 hSBPV/DBPV)、24 h平均收缩压/舒张压(24 h SBP/...目的探讨老年原发性高血压患者血压变异性(BPV)与靶器官损害的关系。方法①收集符合纳入标准的老年人群资料,分为高血压组(n=146)和正常对照组(n=51),比较两组24 h收缩压/舒张压变异性(24 hSBPV/DBPV)、24 h平均收缩压/舒张压(24 h SBP/DBP)、日间收缩压/舒张压变异性(d SBPV/DBPV)及夜间收缩压/舒张压变异性(n SBPV/DBPV);②根据24 h SBPV第50百分位数(P50)将高血压组分为高BPV组和低BPV组,比较高BPV组、低BPV组、正常对照组的血脂、颈总动脉内-中膜厚度(IMT)、左室质量指数(LVMI)、24 h尿微量白蛋白(MA)、合并心血管疾病等指标;③再分别以各危险因素为自变量,IMT、LVMI、MA为因变量进行多因素分析。结果①高血压组24 h SBPV、d SBPV高于对照组(P<0.001,P<0.05)。②高BPV组冠心病、DM、IMT、LVMI、MA、粥样斑块发生率高于低BPV组(P<0.05或P<0.01)。③多因素分析显示,24 h SBPV与IMT、LVMI、MA均有相关关系。结论老年高血压患者BPV增高,BPV是高血压靶器官损伤的重要预测指标。展开更多
文摘Background Catestatin, a chromogranin A-derived peptide, is a potent antagonist of nicotine-evoked catecholamine release. We know that catecholamine plays an important role in cardiovascular remodeling induced by hypertension, therefore we hypothesized that catestatin would affect target-organ structure during hypertension. Methods Twelve spontaneously hypertensive rats （SHRs） were randomized to SHR control group and catestatin group, the normal control group was comprised of six healthy Wistar-Kyoto rats of the same age. Tail-cuff blood pressure and pulse rate were obtained at weeks 1, 4 and 8. At the end of the eight-week period, the heart, abdominal aorta and left kidney were excised and weighed, VG staining was done and the intima-media thickness of vessels and the collagen volume fraction were assessed by an image acquisition and analysis system. The proliferating cell nuclear antigen （PCNA） was observed by immunohistochemistry, and real time reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the mRNA levels of proliferative genes including cyclin A, ki67 and PCNA in the abdominal aorta. Results All the parameters in SHR observed in the present study increased significantly compared to Wistar Kyoto rats （P 〈0.01 ）. With intervention with catestatin, the systolic blood pressure decreased slightly but it was not significantly different from the SHR control, the cardiac mass index and left ventricular mass index both decreased significantly, the collagen volume fraction decreased by nearly 30% in the heart, by 25% in vessels and by 10% in the kidney, and the intima-media thickness and expression of proliferative genes, including cyclin A, ki67 and PCNA, in the abdominal aorta also decreased significantly. Conclusions The present study indicated that catestatin could ameliorate proliferating changes of heart, kidney and vessels during hypertension, especially to the deposition of interstitial collagen. Blood pressure was not the main factor to mediate this effect, which
文摘目的探讨老年原发性高血压患者血压变异性(BPV)与靶器官损害的关系。方法①收集符合纳入标准的老年人群资料,分为高血压组(n=146)和正常对照组(n=51),比较两组24 h收缩压/舒张压变异性(24 hSBPV/DBPV)、24 h平均收缩压/舒张压(24 h SBP/DBP)、日间收缩压/舒张压变异性(d SBPV/DBPV)及夜间收缩压/舒张压变异性(n SBPV/DBPV);②根据24 h SBPV第50百分位数(P50)将高血压组分为高BPV组和低BPV组,比较高BPV组、低BPV组、正常对照组的血脂、颈总动脉内-中膜厚度(IMT)、左室质量指数(LVMI)、24 h尿微量白蛋白(MA)、合并心血管疾病等指标;③再分别以各危险因素为自变量,IMT、LVMI、MA为因变量进行多因素分析。结果①高血压组24 h SBPV、d SBPV高于对照组(P<0.001,P<0.05)。②高BPV组冠心病、DM、IMT、LVMI、MA、粥样斑块发生率高于低BPV组(P<0.05或P<0.01)。③多因素分析显示,24 h SBPV与IMT、LVMI、MA均有相关关系。结论老年高血压患者BPV增高,BPV是高血压靶器官损伤的重要预测指标。
