Genetic mapping provides a powerful tool for the analysis of quantitative trait loci （QTLs） at the genomic level. Herein, we report a new genetic linkage map developed from an F1-derived doubled haploid （DH） popul...Genetic mapping provides a powerful tool for the analysis of quantitative trait loci （QTLs） at the genomic level. Herein, we report a new genetic linkage map developed from an F1-derived doubled haploid （DH） population of 168 lines, which was generated from the cross between two elite Chinese common wheat （Triticum aestivum L.） varieties, Huapei 3 and Yumai 57. The map contained 305 loci, represented by 283 simple sequence repeat （SSR） and 22 expressed sequence tag （EST）-SSR markers, which covered a total length of 2141.7 cM with an average distance of 7.02 cM between adjacent markers on the map. The chromosomal locations and map positions of 22 new SSR markers were determined, and were found to distribute on 14 linkage groups. Twenty SSR loci showed different chromosomal locations from those reported in other maps. Therefore, this map offers new information on the SSR markers of wheat. This genetic map provides new opportunities to detect and map QTLs controlling agronomically important traits. The unique features of this map are discussed.展开更多
基于山核桃Carya cathayensis脂肪代谢相关cDNA文库的部分测序结果,发现43条cDNA序列具有简单重复序列(SSR)碱基重复特点,用Primer Premier 5设计了34对表达序列标签-简单重复序列(EST-SSR)引物;建立了适用于山核桃的SSR分析体系,并用...基于山核桃Carya cathayensis脂肪代谢相关cDNA文库的部分测序结果,发现43条cDNA序列具有简单重复序列(SSR)碱基重复特点,用Primer Premier 5设计了34对表达序列标签-简单重复序列(EST-SSR)引物;建立了适用于山核桃的SSR分析体系,并用设计的SSR引物对对天然群体山核桃40个样品进行了初步的应用性试验。结果表明:34对引物中有4对无扩增产物,3对引物的扩增产物超过了设计范围,27对引物在供试的40个山核桃材料中有较清晰且稳定的目标扩增产物,其中有18对引物的扩增产生了22个多态性位点,占总扩增位点数的47.8%。将SSR标记分析结果与山核桃已有的显性标记分析结果进行比较,发现SSR标记多态性位点的比例较高,且能够区分显性纯合位点及显性杂合位点。结论:设计开发山核桃的EST-SSR标记是可行有效的。展开更多
基金the National Natural Science Foundation of China (30671270)the Hi-Tech Research and Development (863) Program of China(2006AA10Z1E9 and 2006AA100101)
文摘Genetic mapping provides a powerful tool for the analysis of quantitative trait loci （QTLs） at the genomic level. Herein, we report a new genetic linkage map developed from an F1-derived doubled haploid （DH） population of 168 lines, which was generated from the cross between two elite Chinese common wheat （Triticum aestivum L.） varieties, Huapei 3 and Yumai 57. The map contained 305 loci, represented by 283 simple sequence repeat （SSR） and 22 expressed sequence tag （EST）-SSR markers, which covered a total length of 2141.7 cM with an average distance of 7.02 cM between adjacent markers on the map. The chromosomal locations and map positions of 22 new SSR markers were determined, and were found to distribute on 14 linkage groups. Twenty SSR loci showed different chromosomal locations from those reported in other maps. Therefore, this map offers new information on the SSR markers of wheat. This genetic map provides new opportunities to detect and map QTLs controlling agronomically important traits. The unique features of this map are discussed.
文摘基于山核桃Carya cathayensis脂肪代谢相关cDNA文库的部分测序结果,发现43条cDNA序列具有简单重复序列(SSR)碱基重复特点,用Primer Premier 5设计了34对表达序列标签-简单重复序列(EST-SSR)引物;建立了适用于山核桃的SSR分析体系,并用设计的SSR引物对对天然群体山核桃40个样品进行了初步的应用性试验。结果表明:34对引物中有4对无扩增产物,3对引物的扩增产物超过了设计范围,27对引物在供试的40个山核桃材料中有较清晰且稳定的目标扩增产物,其中有18对引物的扩增产生了22个多态性位点,占总扩增位点数的47.8%。将SSR标记分析结果与山核桃已有的显性标记分析结果进行比较,发现SSR标记多态性位点的比例较高,且能够区分显性纯合位点及显性杂合位点。结论:设计开发山核桃的EST-SSR标记是可行有效的。
