In the present study,we prepared nattokinase-loaded self-double-emulsifying drug delivery system(SDEDDS)and investigated its preliminary pharmacodynamics.The type and concentration of oil phase,inner aqueous phase and...In the present study,we prepared nattokinase-loaded self-double-emulsifying drug delivery system(SDEDDS)and investigated its preliminary pharmacodynamics.The type and concentration of oil phase,inner aqueous phase and emulsifier were screened to prepare optimum nattokinase-loaded SDEDDS.Next,the optimum formulations were characterized based on microstructure,volume-weighted mean droplet size,self-emulsifying rate,yield,storage stability,in vitro release and in vivo pharmacodynamics studies.The water/oil/watermultiple emulsions exhibited typicalmultiple structure,with relatively small volumeweighted mean droplet size 6.0±0.7μm and high self-emulsifying ability(self-emulsifying time<2 min).Encapsulation of nattokinase was up to 86.8±8.2%.The cumulative release of nattokinase within 8 h was about 30%,exhibiting a sustained release effect.The pharmacodynamics study indicated that nattokinase-loaded SDEDDS could significantly prolong the whole blood clotting time in mouse and effectively improve the carrageenan-induced tail thrombosis compared with nattokinase solution.Moreover,we showed that SDEDDS could successfully self-emulsify into water/oil/water multiple emulsions upon dilution in dispersion medium with gentle stirring and effectively protect nattokinase activity in gastric environment.Our findings suggested that SDEDDS could be a promising strategy for peptide and protein drugs by oral administration.展开更多
A red water phenomenon occurred in several communities few days after the change of water source in Beijing, China in 2008. In this study, the origin of this problem, the mechanism of iron release and various control ...A red water phenomenon occurred in several communities few days after the change of water source in Beijing, China in 2008. In this study, the origin of this problem, the mechanism of iron release and various control measures were investigated. The results indicated that a significant increase in sulphate concentration as a result of the new water source was the cause of the red water phenomenon. The mechanism of iron release was found that the high-concentration sulphate in the new water source disrupted the stable shell of scale on the inner pipe and led to the release of iron compounds. Experiments showed that the iron release rate in the new source water within pipe section was over 11-fold higher than that occurring within the local source water. The recovery of tap water quality lasted several months despite ameliora- tive measures being implemented, including adding phosphate, reducing the overall proportion of the new water source, elevating the pH and alkalinity, and utilizing free chlorine as a disinfectant instead of chloramine. Adding phosphate was more effective and more practical than the other measures. The iron release rate was decreased after the addition of 1.5 mg. L-1 orthophosphate- P, tripolyphosphate-P and hexametaphosphate-P by 68%, 83% and 87%, respectively. Elevating the pH and alkalinity also reduced the iron release rate by 50%. However, the iron release rate did not decreased after replacing chloramine by 0.5-0.8 mg. L-1 of free chlorine as disinfectant.展开更多
Based on the temperature of the black body (TBB),station observed and NCEP reanalysis data,the impacts of the eastward propagation of convective cloud systems over the Tibetan Plateau on the southwest vortex (SWV) for...Based on the temperature of the black body (TBB),station observed and NCEP reanalysis data,the impacts of the eastward propagation of convective cloud systems over the Tibetan Plateau on the southwest vortex (SWV) formation that occurred at 1800 UTC on 29 June 2003 are analyzed by using the Zwack-Okossi (Z-O) equation to diagnose the thermal and dynamic processes.It is found that,in summer,severe convective activities often occur over the Tibetan Plateau due to the abundant supply of moisture.The convective cloud near the east edge of the plateau could move eastward with a shortwave trough in the westerly.The divergent center that is induced by latent heat release,which is associated with severe convective activities,moves out with the convective cloud and contributes to the low level decompression which is favorable for the formation of plateau edge cyclogenesis (PEC).The Z-O equation indicates that,in this case,the latent heat release and convergence are the two most important factors for SWV formation,which amounts to about 42% and 15% of the term TOTAL,respectively.It is implied that the thermal process effect was more important than the dynamic process during SWV formation.展开更多
基金supported by National Natural Science Foundation of China(No.81373338).
文摘In the present study,we prepared nattokinase-loaded self-double-emulsifying drug delivery system(SDEDDS)and investigated its preliminary pharmacodynamics.The type and concentration of oil phase,inner aqueous phase and emulsifier were screened to prepare optimum nattokinase-loaded SDEDDS.Next,the optimum formulations were characterized based on microstructure,volume-weighted mean droplet size,self-emulsifying rate,yield,storage stability,in vitro release and in vivo pharmacodynamics studies.The water/oil/watermultiple emulsions exhibited typicalmultiple structure,with relatively small volumeweighted mean droplet size 6.0±0.7μm and high self-emulsifying ability(self-emulsifying time<2 min).Encapsulation of nattokinase was up to 86.8±8.2%.The cumulative release of nattokinase within 8 h was about 30%,exhibiting a sustained release effect.The pharmacodynamics study indicated that nattokinase-loaded SDEDDS could significantly prolong the whole blood clotting time in mouse and effectively improve the carrageenan-induced tail thrombosis compared with nattokinase solution.Moreover,we showed that SDEDDS could successfully self-emulsify into water/oil/water multiple emulsions upon dilution in dispersion medium with gentle stirring and effectively protect nattokinase activity in gastric environment.Our findings suggested that SDEDDS could be a promising strategy for peptide and protein drugs by oral administration.
文摘A red water phenomenon occurred in several communities few days after the change of water source in Beijing, China in 2008. In this study, the origin of this problem, the mechanism of iron release and various control measures were investigated. The results indicated that a significant increase in sulphate concentration as a result of the new water source was the cause of the red water phenomenon. The mechanism of iron release was found that the high-concentration sulphate in the new water source disrupted the stable shell of scale on the inner pipe and led to the release of iron compounds. Experiments showed that the iron release rate in the new source water within pipe section was over 11-fold higher than that occurring within the local source water. The recovery of tap water quality lasted several months despite ameliora- tive measures being implemented, including adding phosphate, reducing the overall proportion of the new water source, elevating the pH and alkalinity, and utilizing free chlorine as a disinfectant instead of chloramine. Adding phosphate was more effective and more practical than the other measures. The iron release rate was decreased after the addition of 1.5 mg. L-1 orthophosphate- P, tripolyphosphate-P and hexametaphosphate-P by 68%, 83% and 87%, respectively. Elevating the pH and alkalinity also reduced the iron release rate by 50%. However, the iron release rate did not decreased after replacing chloramine by 0.5-0.8 mg. L-1 of free chlorine as disinfectant.
基金supported by the National Natural Science Foundation of China (Grant Nos. 40875021 and 40930951)the project of the State Key Laboratory of Severe Weather,Chinese Academy of Meteorological Sciences (Grant No. 2009LASW-A03)
文摘Based on the temperature of the black body (TBB),station observed and NCEP reanalysis data,the impacts of the eastward propagation of convective cloud systems over the Tibetan Plateau on the southwest vortex (SWV) formation that occurred at 1800 UTC on 29 June 2003 are analyzed by using the Zwack-Okossi (Z-O) equation to diagnose the thermal and dynamic processes.It is found that,in summer,severe convective activities often occur over the Tibetan Plateau due to the abundant supply of moisture.The convective cloud near the east edge of the plateau could move eastward with a shortwave trough in the westerly.The divergent center that is induced by latent heat release,which is associated with severe convective activities,moves out with the convective cloud and contributes to the low level decompression which is favorable for the formation of plateau edge cyclogenesis (PEC).The Z-O equation indicates that,in this case,the latent heat release and convergence are the two most important factors for SWV formation,which amounts to about 42% and 15% of the term TOTAL,respectively.It is implied that the thermal process effect was more important than the dynamic process during SWV formation.
