Plant natural products(PNPs)are the main sources of drugs,food additives,and new biofuels and have become a hotspot in synthetic biology.In the past two decades,the engineered biosynthesis of many PNPs has been achiev...Plant natural products(PNPs)are the main sources of drugs,food additives,and new biofuels and have become a hotspot in synthetic biology.In the past two decades,the engineered biosynthesis of many PNPs has been achieved through the construction of microbial cell factories.Alongside the rapid development of plant physiology,genetics,and plant genetic modification techniques,hosts have now expanded from single-celled microbes to complex plant systems.Plant synthetic biology is an emerging field that combines engineering principles with plant biology.In this review,we introduce recent advances in the biosynthetic pathway elucidation of PNPs and summarize the progress of engineered PNP biosynthesis in plant cells.Furthermore,a future vision of plant synthetic biology is proposed.Although we are still a long way from overcoming all the bottlenecks in plant synthetic biology,the ascent of this field is expected to provide a huge opportunity for future agriculture and industry.展开更多
植物合成生物学为解决农作物育种及品质改良等重要农业问题带来新机遇。为了使相关科研和管理人员及时了解植物合成生物学的研究进展、把握领域科技前沿、有效开展科研合作、优化研究布局,从文献计量角度分析合成生物学的发展态势。基于...植物合成生物学为解决农作物育种及品质改良等重要农业问题带来新机遇。为了使相关科研和管理人员及时了解植物合成生物学的研究进展、把握领域科技前沿、有效开展科研合作、优化研究布局,从文献计量角度分析合成生物学的发展态势。基于Web of Science核心集检索到2000—2020年研究文献97551篇。计量分析结果表明,植物合成生物学领域论文呈现稳定增长趋势,中国学者发表的论文数量及总被引频次位居前列。通过对高被引文献以及文献主题聚类分析可知,重要作用元件如转录因子的调控机制、标准化的遗传元件如启动子、功能基因的挖掘和鉴定、植物天然产物合成与代谢通路的解析、基因编辑技术/多组学分析技术等关键技术是本领域的重要主题内容,将持续推动本领域的创新发展。此外,对比分析了国内外合成生物学的资助及研究布局,针对当前我国在植物合成生物学领域的资助现状提出展望,加强国家顶层设计,加大植物合成生物领域的资助。展开更多
Background:Synthetic biology has attracted enorm ous attention in recent years.A key focus of synthetic biology is to utilize m odular biological building blocks to assem ble the cell-based circuits.Results'.Scien...Background:Synthetic biology has attracted enorm ous attention in recent years.A key focus of synthetic biology is to utilize m odular biological building blocks to assem ble the cell-based circuits.Results'.Scientists have program m ed the living organism s using these circuits to attain m ultiple,delicate and welldefined functions.W ith the integration of tools o r technologies from other disciplines,these rew ired cells can achieve even m ore complex tasks.Conclusions:In this review,we will focus on the recent achievements in new m aterials and devices assembly,next generation therapeutics developm ent and versatile m anufacturing by com bining the synthetic gene circuits,various tools and technologies from multiple fields,such as printing technology,m aterial engineering and electronic engineering.展开更多
基金supported by grants from the National Natural Science Foundation of China(grant no.31901026)the China Postdoctoral Science Foundation(grant no.2019M661032)Tianjin Science and technology plan project(grant no.19PTZWHZ00060).
文摘Plant natural products(PNPs)are the main sources of drugs,food additives,and new biofuels and have become a hotspot in synthetic biology.In the past two decades,the engineered biosynthesis of many PNPs has been achieved through the construction of microbial cell factories.Alongside the rapid development of plant physiology,genetics,and plant genetic modification techniques,hosts have now expanded from single-celled microbes to complex plant systems.Plant synthetic biology is an emerging field that combines engineering principles with plant biology.In this review,we introduce recent advances in the biosynthetic pathway elucidation of PNPs and summarize the progress of engineered PNP biosynthesis in plant cells.Furthermore,a future vision of plant synthetic biology is proposed.Although we are still a long way from overcoming all the bottlenecks in plant synthetic biology,the ascent of this field is expected to provide a huge opportunity for future agriculture and industry.
文摘植物合成生物学为解决农作物育种及品质改良等重要农业问题带来新机遇。为了使相关科研和管理人员及时了解植物合成生物学的研究进展、把握领域科技前沿、有效开展科研合作、优化研究布局,从文献计量角度分析合成生物学的发展态势。基于Web of Science核心集检索到2000—2020年研究文献97551篇。计量分析结果表明,植物合成生物学领域论文呈现稳定增长趋势,中国学者发表的论文数量及总被引频次位居前列。通过对高被引文献以及文献主题聚类分析可知,重要作用元件如转录因子的调控机制、标准化的遗传元件如启动子、功能基因的挖掘和鉴定、植物天然产物合成与代谢通路的解析、基因编辑技术/多组学分析技术等关键技术是本领域的重要主题内容,将持续推动本领域的创新发展。此外,对比分析了国内外合成生物学的资助及研究布局,针对当前我国在植物合成生物学领域的资助现状提出展望,加强国家顶层设计,加大植物合成生物领域的资助。
基金We thank Lingchong You for insightful comments and suggestions.This study was partially supported by the National Key Research and Development Program of China(No.2018YFA0903000)Guangdong Provincial Key Laboratory of Synthetic Genomics(2019B030301006)+1 种基金Shenzhen Key Laboratory of Synthetic Genomics(ZDSYS201802061806209)Shenzhen Science and Technology Program(No.KQTD20180413181837372).
文摘Background:Synthetic biology has attracted enorm ous attention in recent years.A key focus of synthetic biology is to utilize m odular biological building blocks to assem ble the cell-based circuits.Results'.Scientists have program m ed the living organism s using these circuits to attain m ultiple,delicate and welldefined functions.W ith the integration of tools o r technologies from other disciplines,these rew ired cells can achieve even m ore complex tasks.Conclusions:In this review,we will focus on the recent achievements in new m aterials and devices assembly,next generation therapeutics developm ent and versatile m anufacturing by com bining the synthetic gene circuits,various tools and technologies from multiple fields,such as printing technology,m aterial engineering and electronic engineering.
