A new genus and species,Cretopleciofungivora simpsoni gen.et sp.nov.,from the extinct family Pleciofungivoridae(Diptera:Bibionomorpha),is discovered in mid-Cretaceous Kachin amber.Previously,this family was known only...A new genus and species,Cretopleciofungivora simpsoni gen.et sp.nov.,from the extinct family Pleciofungivoridae(Diptera:Bibionomorpha),is discovered in mid-Cretaceous Kachin amber.Previously,this family was known only from imprints in sedimentary rocks of the Jurassic and the Lower Cretaceous.Discovery of a representative of Pleciofungivoridae in Kachin amber confirms the presence of the family in the Upper Cretaceous.The new species has a unique structure of fore tarsus,with lobed and extended tarsal segments Ⅱ to Ⅳ,a feature hitherto known only in a few species of extant Sciaroidea.Although not particularly rare,the new species is currently known only from female specimens.Possible reasons for this phenomenon,very unusual in Sciaroidea,are briefly discussed,including parthenogenesis as a potentially plausible hypothesis.展开更多
[ Objective] To study the effects of clindamycin on germ cells of male mice. [Method] A total of 48 healthy adult male mice were randomly divided into group A, group B, group C and control group, 12 mice in each group...[ Objective] To study the effects of clindamycin on germ cells of male mice. [Method] A total of 48 healthy adult male mice were randomly divided into group A, group B, group C and control group, 12 mice in each group. The mice in the group A, group B and group C were respectively administrated with clindamycin at doses of 15, 30 and 60 mg/kg.d via intraperitoneai injection, and those in the control group were trea- ted with normal saline. On Day 29 after administration, the mice were killed by dislocation, and the sperm motility rate, sperm deformity rate and the percentage of sperm having swollen tail membrane were determined, respectively. [ Result] The sperm motility rate and the percentage of sperm having swollen tail membrane of the group B and group C were lower than those of the control group; and the sperm deformity rate of the group B and group C was higher than that of the control group. The sperm motility rate, sperm deformity rate and percentage of sperm having swollen tail membrane had no significant difference between the group A and control group. [ Conclusion] The clindamycin at a dose equal to or more than that used for severe infection affects the normal function of sperm in mice.展开更多
A 75-year-old man presented with knee pain due to medial osteoarthritis of the knee in the orthopedic outpatient clinic. Conservative treatment was started with steroid infiltration. Besides his knee complaint reporte...A 75-year-old man presented with knee pain due to medial osteoarthritis of the knee in the orthopedic outpatient clinic. Conservative treatment was started with steroid infiltration. Besides his knee complaint reported a bilateral painless swollen calf muscle without traumatic cause, and also without any pain at night, fever or medical illness. On physical examination the soleus muscle had a swollen aspect in both calfs. The skin appeared normal without deformities and the arterial pulsations were intact. An X-ray did not show abnormalities in the tibia. Magnetic resonance imaging of the legs revealed bilateral multiple saccular intramuscular venous malformations involving the soleus muscle. Intramuscular venous malformations in skeletal muscles are rare, especially when the occurrence is bilateral. Bilateral venous malformations have the potential to be missed because of the intramuscular localization. Symptoms of intramuscular venous malformation can be often mild and overlap with nonexercise related compartment syndrome, claudication, lymphedema and post thrombotic syndrome or muscle strains.展开更多
The bacterial swollen stem canker of poplar is prevalent in planted poplars of northeast China. Serious damage occurs in the form of necrosis, putridity, cankers, swollen stems and discolored wood of trunks and branch...The bacterial swollen stem canker of poplar is prevalent in planted poplars of northeast China. Serious damage occurs in the form of necrosis, putridity, cankers, swollen stems and discolored wood of trunks and branches of young and mature trees. Tests of allergic reaction, pathogenicity, culture characteristic, Gram stain and anaerobic culture revealed that the pathogen is Erwinia sp. Its occurrence depends mainly on the predisposing factors of the disease among which the susceptibility of poplars is the most important one. The disease becomes widespread in association with low-lying and high-humidity terrain, frost injury, node wounds, insect damage, and pruning and other mechanical wounds. Harmful factors promote the disease.展开更多
Objective:To observe the clinical efficacy of swollen boil treated by cutaneous region drainage.Methods:35 swollen boil patients were selected and treated with cutaneous region bleeding drainage by applying bloodletti...Objective:To observe the clinical efficacy of swollen boil treated by cutaneous region drainage.Methods:35 swollen boil patients were selected and treated with cutaneous region bleeding drainage by applying bloodletting first and then cupping on the head of boil part. A course of treatment included 3 times, and the efficacy was assessed after 2 courses.Results:After treatment, 30 clinical cases were cured, 4 improved, and 1 unhealed. The total effective rate was 97.1%.Conclusions:Significant therapeutic effect of the cutaneous region drainage was found in curing swollen boil.展开更多
[Objective] To develop an indirect sandwich ELISA for rapid detection of duck swollen head septicemia virus （DSHSV）. [Method] DSHSV hyperimmune sara were prepared in ducks and rabbits by inoculation with DSHSV XD st...[Objective] To develop an indirect sandwich ELISA for rapid detection of duck swollen head septicemia virus （DSHSV）. [Method] DSHSV hyperimmune sara were prepared in ducks and rabbits by inoculation with DSHSV XD strain and then purified using saturated ammonium sulfate and Sephadex G-150 column chromatography to obtain anti-DSHSV IgG. An indirect sandwich ELISA was developed using the purified duck anti-DSHSV IgG and rabbit anti-DSHSV IgG after reaction conditions were optimized. Its specificity, sensitivity and repeatability were evaluated, and its accuracy was confirmed by observation with immunoelectron microscopy. Then, distribution of DSHSV in tissues of challenged ducks was also detected. [Result] Through optimizing conditions, the ELISA was developed. Only DSHSV could be detected by the developed method, but other pathogens could not be detected. Compared with agar gel diffusion test, the developed method was more sensitive. The coefficient of variation was less than 10%, and the developed method had good repeatability. In addition, the ELISA-positive samples contained DSHSV, as confirmed by im- munoelectron microscopy. All heart, liver, lung and kidney collected from the DSHSV-challenged ducks were positive when they were detected by the developed ELISA. [ Conclusion] The developed ELISA method is rapid, simple, specific and sensitive, and it is suitable for large-scale quaran- tine of DSHS. Heart, liver, lunq and kidney should be selected preferentially as specimens for diaclnosis of DSHS.展开更多
Tumorous stem mustard is well known for its swollen stem from which pickled“Fuling Mustard”is made.The molecular mechanisms governing the formation of the modified swollen stems are still poorly understood.This pape...Tumorous stem mustard is well known for its swollen stem from which pickled“Fuling Mustard”is made.The molecular mechanisms governing the formation of the modified swollen stems are still poorly understood.This paper aims to identify candidate genes involved in the developmental regulation of the swollen stems.We sought to map previously published transcriptome datasets for Brassica juncea,including those derived from swollen stems at four different developmental stages and a mutant variety without swollen stems.Using pairwise comparisons of the five datasets,we identified 31368 differentially expressed genes(DEGs).A total of 55 DEGs related to plant hormone signal transduction and 259 continuously up-or downregulated transcription factors were identified during stem development using Gene Ontology(GO)analysis.Quantitative real-time PCR(qRT-PCR)results showed that the expressions of 12 important candidate DEGs were consistent with RNA-seq results.Our study provided digital gene expression profiling and a dynamic view of the swollen stem development process.Furthermore,we identified candidate genes for further studies on mechanisms of modified stem development in non-model species.展开更多
Poly(styrene-glycidyl methacrylate) particles having bowl-shaped hollow structures were synthesized by swollen seed emulsion polymerization. The PS emulsion synthesized through soap-free emulsion polymerization was sw...Poly(styrene-glycidyl methacrylate) particles having bowl-shaped hollow structures were synthesized by swollen seed emulsion polymerization. The PS emulsion synthesized through soap-free emulsion polymerization was swollen by toluene, and then the mixture of second monomers was added under polymerization condition. So a thin shell of poly(styrene-glycidyl methacrylate) cross-linked by triethylene glycol diacrylate was formed around the swollen PS particle. The bowl-shaped particles were obtained after the collapsing of the thin shell when the toluene emanated from the particles, but the shapes were effected by the degrees of cross-linking. The shape of the particles was observed by SEM. The release behavior of solvent from the particles was examined by TG.展开更多
A gram-positive facultatively anaerobic bacterium, named Sporolactobacillus GD201215, was isolated from swollen fruit jelly. Colonies were circular, bulgy, opaque, moist and white, with a 1-2 mm diameter. Its sequence...A gram-positive facultatively anaerobic bacterium, named Sporolactobacillus GD201215, was isolated from swollen fruit jelly. Colonies were circular, bulgy, opaque, moist and white, with a 1-2 mm diameter. Its sequence showed 97.46% and96.91% homology with Sporolactobacillus vineae SL153 T and S. putidus QC81-06 T, respectively. Sporolactobacillus GD201215 uses glucose, fructose and sucrose to produce acid, and metabolizes sodium citrate to generate CO_2 which is responsible for swelling of fruit jelly. It grew anaerobically in GYP broth at 37 ℃, p H 3.5-7.0, with 0-2%(w/v) Na Cl, and could be killed by heating at 71 ℃ for above 5 min.展开更多
A 27-year-old male started to have his ankles swollen during his military service. He was examined at a military hospital where electromyoneurography showed the signs of distal sensory-motor polyneuropathy with axon d...A 27-year-old male started to have his ankles swollen during his military service. He was examined at a military hospital where electromyoneurography showed the signs of distal sensory-motor polyneuropathy with axon demyelinization and weak myopathic changes, whereas histopathological examination of gastrocnemius muscle biopsy revealed some mild and nonspecific myopathy. Besides, he was found to have subcutaneous ankle tissue edemas and hypertransaminasemia. Due to these reasons, he was dismissed from the military service and examined at another hospital where bone osteodensitometry revealed low bone mineral density of the spine. However, his medical problems were not resolved and after the second discharge from hospital he was desperately seeing doctors from time to time. Finally, at our institution he was shown to have celiac disease (CD) by positive serology (antitissue transglutaminase and antiendomysial antibodies) and small bowel mucosal histopathological examination, which showed total small bowel villous atrophy. Three months after the initiation of gluten-free diet, his ankle edema disappeared, electromyoneurographic signs of polyneuropathy improved and liver aminotransferases normalized. Good knowledge of CD extraintestinal signs and serologic screening are essential for early CD recognition and therapy.展开更多
基金funded by the Science Foundation of Yunnan Province(Grant Nos.2015HA021 and 202401CF070913)the National Science Centre of Poland(Grant No.UMO-2016/23/B/NZ8/00936)supported by the Alexander von Humboldt Foundation。
文摘A new genus and species,Cretopleciofungivora simpsoni gen.et sp.nov.,from the extinct family Pleciofungivoridae(Diptera:Bibionomorpha),is discovered in mid-Cretaceous Kachin amber.Previously,this family was known only from imprints in sedimentary rocks of the Jurassic and the Lower Cretaceous.Discovery of a representative of Pleciofungivoridae in Kachin amber confirms the presence of the family in the Upper Cretaceous.The new species has a unique structure of fore tarsus,with lobed and extended tarsal segments Ⅱ to Ⅳ,a feature hitherto known only in a few species of extant Sciaroidea.Although not particularly rare,the new species is currently known only from female specimens.Possible reasons for this phenomenon,very unusual in Sciaroidea,are briefly discussed,including parthenogenesis as a potentially plausible hypothesis.
基金supported by the grants from the University Scientific Research Project of Inner Mongolia Autonomous Region (NJzc08159)
文摘[ Objective] To study the effects of clindamycin on germ cells of male mice. [Method] A total of 48 healthy adult male mice were randomly divided into group A, group B, group C and control group, 12 mice in each group. The mice in the group A, group B and group C were respectively administrated with clindamycin at doses of 15, 30 and 60 mg/kg.d via intraperitoneai injection, and those in the control group were trea- ted with normal saline. On Day 29 after administration, the mice were killed by dislocation, and the sperm motility rate, sperm deformity rate and the percentage of sperm having swollen tail membrane were determined, respectively. [ Result] The sperm motility rate and the percentage of sperm having swollen tail membrane of the group B and group C were lower than those of the control group; and the sperm deformity rate of the group B and group C was higher than that of the control group. The sperm motility rate, sperm deformity rate and percentage of sperm having swollen tail membrane had no significant difference between the group A and control group. [ Conclusion] The clindamycin at a dose equal to or more than that used for severe infection affects the normal function of sperm in mice.
文摘A 75-year-old man presented with knee pain due to medial osteoarthritis of the knee in the orthopedic outpatient clinic. Conservative treatment was started with steroid infiltration. Besides his knee complaint reported a bilateral painless swollen calf muscle without traumatic cause, and also without any pain at night, fever or medical illness. On physical examination the soleus muscle had a swollen aspect in both calfs. The skin appeared normal without deformities and the arterial pulsations were intact. An X-ray did not show abnormalities in the tibia. Magnetic resonance imaging of the legs revealed bilateral multiple saccular intramuscular venous malformations involving the soleus muscle. Intramuscular venous malformations in skeletal muscles are rare, especially when the occurrence is bilateral. Bilateral venous malformations have the potential to be missed because of the intramuscular localization. Symptoms of intramuscular venous malformation can be often mild and overlap with nonexercise related compartment syndrome, claudication, lymphedema and post thrombotic syndrome or muscle strains.
文摘The bacterial swollen stem canker of poplar is prevalent in planted poplars of northeast China. Serious damage occurs in the form of necrosis, putridity, cankers, swollen stems and discolored wood of trunks and branches of young and mature trees. Tests of allergic reaction, pathogenicity, culture characteristic, Gram stain and anaerobic culture revealed that the pathogen is Erwinia sp. Its occurrence depends mainly on the predisposing factors of the disease among which the susceptibility of poplars is the most important one. The disease becomes widespread in association with low-lying and high-humidity terrain, frost injury, node wounds, insect damage, and pruning and other mechanical wounds. Harmful factors promote the disease.
基金Supported by The Key Project of Natural Science Foundation of Anhui University of Chinese Medicine in 2017:2017zrzd002
文摘Objective:To observe the clinical efficacy of swollen boil treated by cutaneous region drainage.Methods:35 swollen boil patients were selected and treated with cutaneous region bleeding drainage by applying bloodletting first and then cupping on the head of boil part. A course of treatment included 3 times, and the efficacy was assessed after 2 courses.Results:After treatment, 30 clinical cases were cured, 4 improved, and 1 unhealed. The total effective rate was 97.1%.Conclusions:Significant therapeutic effect of the cutaneous region drainage was found in curing swollen boil.
基金supported by Program for Changjiang Scholars and Innovative Research Team in University (IRT0848)
文摘[Objective] To develop an indirect sandwich ELISA for rapid detection of duck swollen head septicemia virus （DSHSV）. [Method] DSHSV hyperimmune sara were prepared in ducks and rabbits by inoculation with DSHSV XD strain and then purified using saturated ammonium sulfate and Sephadex G-150 column chromatography to obtain anti-DSHSV IgG. An indirect sandwich ELISA was developed using the purified duck anti-DSHSV IgG and rabbit anti-DSHSV IgG after reaction conditions were optimized. Its specificity, sensitivity and repeatability were evaluated, and its accuracy was confirmed by observation with immunoelectron microscopy. Then, distribution of DSHSV in tissues of challenged ducks was also detected. [Result] Through optimizing conditions, the ELISA was developed. Only DSHSV could be detected by the developed method, but other pathogens could not be detected. Compared with agar gel diffusion test, the developed method was more sensitive. The coefficient of variation was less than 10%, and the developed method had good repeatability. In addition, the ELISA-positive samples contained DSHSV, as confirmed by im- munoelectron microscopy. All heart, liver, lung and kidney collected from the DSHSV-challenged ducks were positive when they were detected by the developed ELISA. [ Conclusion] The developed ELISA method is rapid, simple, specific and sensitive, and it is suitable for large-scale quaran- tine of DSHS. Heart, liver, lunq and kidney should be selected preferentially as specimens for diaclnosis of DSHS.
基金supported by the National Natural Science Foundation of China(Grant No.31701928)the Chongqing Natural Science Foundation(Grant No.cstc2017jcyjAX0226)+1 种基金the Science and Technology Research Program of Chongqing Municipal Education Commission(Grant No.KJ1712305)research grants of Yangtze Normal University(Grant Nos.2016XJQN07,2016KYQD19).
文摘Tumorous stem mustard is well known for its swollen stem from which pickled“Fuling Mustard”is made.The molecular mechanisms governing the formation of the modified swollen stems are still poorly understood.This paper aims to identify candidate genes involved in the developmental regulation of the swollen stems.We sought to map previously published transcriptome datasets for Brassica juncea,including those derived from swollen stems at four different developmental stages and a mutant variety without swollen stems.Using pairwise comparisons of the five datasets,we identified 31368 differentially expressed genes(DEGs).A total of 55 DEGs related to plant hormone signal transduction and 259 continuously up-or downregulated transcription factors were identified during stem development using Gene Ontology(GO)analysis.Quantitative real-time PCR(qRT-PCR)results showed that the expressions of 12 important candidate DEGs were consistent with RNA-seq results.Our study provided digital gene expression profiling and a dynamic view of the swollen stem development process.Furthermore,we identified candidate genes for further studies on mechanisms of modified stem development in non-model species.
文摘Poly(styrene-glycidyl methacrylate) particles having bowl-shaped hollow structures were synthesized by swollen seed emulsion polymerization. The PS emulsion synthesized through soap-free emulsion polymerization was swollen by toluene, and then the mixture of second monomers was added under polymerization condition. So a thin shell of poly(styrene-glycidyl methacrylate) cross-linked by triethylene glycol diacrylate was formed around the swollen PS particle. The bowl-shaped particles were obtained after the collapsing of the thin shell when the toluene emanated from the particles, but the shapes were effected by the degrees of cross-linking. The shape of the particles was observed by SEM. The release behavior of solvent from the particles was examined by TG.
文摘A gram-positive facultatively anaerobic bacterium, named Sporolactobacillus GD201215, was isolated from swollen fruit jelly. Colonies were circular, bulgy, opaque, moist and white, with a 1-2 mm diameter. Its sequence showed 97.46% and96.91% homology with Sporolactobacillus vineae SL153 T and S. putidus QC81-06 T, respectively. Sporolactobacillus GD201215 uses glucose, fructose and sucrose to produce acid, and metabolizes sodium citrate to generate CO_2 which is responsible for swelling of fruit jelly. It grew anaerobically in GYP broth at 37 ℃, p H 3.5-7.0, with 0-2%(w/v) Na Cl, and could be killed by heating at 71 ℃ for above 5 min.
文摘A 27-year-old male started to have his ankles swollen during his military service. He was examined at a military hospital where electromyoneurography showed the signs of distal sensory-motor polyneuropathy with axon demyelinization and weak myopathic changes, whereas histopathological examination of gastrocnemius muscle biopsy revealed some mild and nonspecific myopathy. Besides, he was found to have subcutaneous ankle tissue edemas and hypertransaminasemia. Due to these reasons, he was dismissed from the military service and examined at another hospital where bone osteodensitometry revealed low bone mineral density of the spine. However, his medical problems were not resolved and after the second discharge from hospital he was desperately seeing doctors from time to time. Finally, at our institution he was shown to have celiac disease (CD) by positive serology (antitissue transglutaminase and antiendomysial antibodies) and small bowel mucosal histopathological examination, which showed total small bowel villous atrophy. Three months after the initiation of gluten-free diet, his ankle edema disappeared, electromyoneurographic signs of polyneuropathy improved and liver aminotransferases normalized. Good knowledge of CD extraintestinal signs and serologic screening are essential for early CD recognition and therapy.
