Metal-organic framework(MoF)with a buildable internal structure has aroused great interest focus as self-sacrificing precursors of porous carbon(PC).However,as a drug carrier,the MOF-derived PC developed thus far are ...Metal-organic framework(MoF)with a buildable internal structure has aroused great interest focus as self-sacrificing precursors of porous carbon(PC).However,as a drug carrier,the MOF-derived PC developed thus far are generally composed of irregular powder shape due to their crystalline nature,which consequently causing the cerebral infarction,cerebral thrombosis,and other blood diseases.In this article,we propose a novel approach to constructing amorphous carbon microspheres(AcMs)by dis-torting the topological network through hydrothermal treatment precursors of MIL-101(Fe).Then,a distinctive MIL-101(Fe)-derived spherical porous carbons(MSPC)is achieved through high temperature calcination toward ACMs.Effects of the glucose initial concentration and hydrothermal treatment time on the sphericity of the as-prepared mesoporous MsPC were investigated in depth.And the loading capacities and sustained-release performances of nitroimidazole drugs over MsPC through simulation internal environment of human body at different pH values was systematically evaluated.The nitro-imidazole drugs loading rate and release time of MsPC are 10% and 17 h under preferred process.Furthermore,the MSPC exhibited very low toxicity on Hela cells and 293T cells at the concentrations tested(10-800μg mL^(-1)).This study,therefore,supports the potential of the mesoporous carbon spheres as a carrierfor nitroimidazole drug delivery.展开更多
The contents of indapamide and related impurities in generic indapamide sustained-release tablets were simultaneously detected by a single-run high performance liquid chromatography equipped with photodiode array dete...The contents of indapamide and related impurities in generic indapamide sustained-release tablets were simultaneously detected by a single-run high performance liquid chromatography equipped with photodiode array detector(HPLC-PDA)method for the quality control in this paper.The results showed the method had a good selectivity and was validated through linearity,limits of detection and quantification,recovery,and precision.The linear ranges of indapamide,2-methyl-1-nitroso-2,3-dihydro-1H-indole(impurity A,ImA),4-chloro-N-(2-methyl-1H-indol-1-yl)-3-sulphamoyl-benzamide(impurity B,ImB)and 4-chloro-3-sulfamoylbenzoic acid(impurity 1,Im1)were 0.028-1.80μg/mL(R=0.99995),0.060-1.20μg/mL(R=0.9996),0.0324-1.20μg/mL(R=0.99985)and 0.060-1.20μg/mL(R=0.9997)with detection limits of 0.0093,0.012,0.012 and 0.006μg/mL,respectively.ImA and Im1 were not detectable in the generic drug.The content of indapamide was 96.7%of the labeled amount with a relative standard deviation(RSD)of 1.30%,and the percentage of ImB relative to the labeled amounts of indapamide was 0.106%with an RSD of 1.82%.The content of other unspecified impurities all met the reference quality standards.The results provided references for the quality control and the quality standard study of generic indapamide sustained-release tablets.展开更多
基金the National Natural Science Foundation of China(grant No.22178325).
文摘Metal-organic framework(MoF)with a buildable internal structure has aroused great interest focus as self-sacrificing precursors of porous carbon(PC).However,as a drug carrier,the MOF-derived PC developed thus far are generally composed of irregular powder shape due to their crystalline nature,which consequently causing the cerebral infarction,cerebral thrombosis,and other blood diseases.In this article,we propose a novel approach to constructing amorphous carbon microspheres(AcMs)by dis-torting the topological network through hydrothermal treatment precursors of MIL-101(Fe).Then,a distinctive MIL-101(Fe)-derived spherical porous carbons(MSPC)is achieved through high temperature calcination toward ACMs.Effects of the glucose initial concentration and hydrothermal treatment time on the sphericity of the as-prepared mesoporous MsPC were investigated in depth.And the loading capacities and sustained-release performances of nitroimidazole drugs over MsPC through simulation internal environment of human body at different pH values was systematically evaluated.The nitro-imidazole drugs loading rate and release time of MsPC are 10% and 17 h under preferred process.Furthermore,the MSPC exhibited very low toxicity on Hela cells and 293T cells at the concentrations tested(10-800μg mL^(-1)).This study,therefore,supports the potential of the mesoporous carbon spheres as a carrierfor nitroimidazole drug delivery.
基金the Natural Science Foundation of Anhui Province,China(11040606M41)the Natural Science Project in Colleges and Universities of Anhui Province,China(kj2016A683)the Natural Science Project of Huangshan University,China(hxkt20190042,xdhz202007)。
文摘The contents of indapamide and related impurities in generic indapamide sustained-release tablets were simultaneously detected by a single-run high performance liquid chromatography equipped with photodiode array detector(HPLC-PDA)method for the quality control in this paper.The results showed the method had a good selectivity and was validated through linearity,limits of detection and quantification,recovery,and precision.The linear ranges of indapamide,2-methyl-1-nitroso-2,3-dihydro-1H-indole(impurity A,ImA),4-chloro-N-(2-methyl-1H-indol-1-yl)-3-sulphamoyl-benzamide(impurity B,ImB)and 4-chloro-3-sulfamoylbenzoic acid(impurity 1,Im1)were 0.028-1.80μg/mL(R=0.99995),0.060-1.20μg/mL(R=0.9996),0.0324-1.20μg/mL(R=0.99985)and 0.060-1.20μg/mL(R=0.9997)with detection limits of 0.0093,0.012,0.012 and 0.006μg/mL,respectively.ImA and Im1 were not detectable in the generic drug.The content of indapamide was 96.7%of the labeled amount with a relative standard deviation(RSD)of 1.30%,and the percentage of ImB relative to the labeled amounts of indapamide was 0.106%with an RSD of 1.82%.The content of other unspecified impurities all met the reference quality standards.The results provided references for the quality control and the quality standard study of generic indapamide sustained-release tablets.
