Genetically encoded covalent peptide tagging tools such as the SpyTag/SpyCatcher reactive pair,have been demonstrated versatile and useful for protein modification.Herein,we present a superpositively charged SpyCatche...Genetically encoded covalent peptide tagging tools such as the SpyTag/SpyCatcher reactive pair,have been demonstrated versatile and useful for protein modification.Herein,we present a superpositively charged SpyCatcher bearing a theoretical net charge of+21 capable of accomplishing multiple unrelated independent tasks to enrich this toolbox and cultivate new functions.The SpyCatcher(+21)possessed stimuli-responsive reactivity toward SpyTag and could serve as a potent and general platform for the delivery of proteins,including RNaseAinto HeLa cells.Remarkably,the delivered RNase A caused substantial proliferation inhibition toward HeLa cells.In addition,the superpositively charged SpyCatcher could form coacervate with plasmid DNA for further study of gene delivery and liquid–liquid phase separation.These findings demonstrate the robustness of the SpyTag/SpyCatcher structure against surface mutation and the prospect of applying supercharging technology on diverse functional proteins to create moonlighting proteins.展开更多
Supercharging is the process of supplying air for combustion at a pressure greater than that achieved by natural or atmospheric induction, as applied to internal combustion engines. As a consequence of demonstrated te...Supercharging is the process of supplying air for combustion at a pressure greater than that achieved by natural or atmospheric induction, as applied to internal combustion engines. As a consequence of demonstrated technological, economical and energetic advantages in multiple literature evaluations concerning the large scale wind-compressed air hybrid storage system with gas turbines, the utilization of a hybrid wind-diesel system with compressed air storage (HWDCAS) has been frequently explored. These will mainly have average or small scale application such as the powering of isolated sites. It has been proven in numerous studies that the HWDCAS combined with an additional supercharging of the diesel engines will contribute to the increase of the power and efficiency of the diesel engine, the reduction of both fuel consumption and the emission of greenhouse gases (GHG). This article presents the obtained results from experimental validation of the selected design with an aim to valorize this innovative solution and become trustworthy.展开更多
基金support fromthe National Key R&D Program of China(grant nos.2020YFA0908100,2020AAA0105200)the National Natural Science Foundation of China(NSFC,grant nos.21991132,21925102,92056118,8200907120,8200907121)Beijing National Laboratory for Molecular Sciences(BNLMS,grant no.BNLMS-CXXM-202006).
文摘Genetically encoded covalent peptide tagging tools such as the SpyTag/SpyCatcher reactive pair,have been demonstrated versatile and useful for protein modification.Herein,we present a superpositively charged SpyCatcher bearing a theoretical net charge of+21 capable of accomplishing multiple unrelated independent tasks to enrich this toolbox and cultivate new functions.The SpyCatcher(+21)possessed stimuli-responsive reactivity toward SpyTag and could serve as a potent and general platform for the delivery of proteins,including RNaseAinto HeLa cells.Remarkably,the delivered RNase A caused substantial proliferation inhibition toward HeLa cells.In addition,the superpositively charged SpyCatcher could form coacervate with plasmid DNA for further study of gene delivery and liquid–liquid phase separation.These findings demonstrate the robustness of the SpyTag/SpyCatcher structure against surface mutation and the prospect of applying supercharging technology on diverse functional proteins to create moonlighting proteins.
文摘Supercharging is the process of supplying air for combustion at a pressure greater than that achieved by natural or atmospheric induction, as applied to internal combustion engines. As a consequence of demonstrated technological, economical and energetic advantages in multiple literature evaluations concerning the large scale wind-compressed air hybrid storage system with gas turbines, the utilization of a hybrid wind-diesel system with compressed air storage (HWDCAS) has been frequently explored. These will mainly have average or small scale application such as the powering of isolated sites. It has been proven in numerous studies that the HWDCAS combined with an additional supercharging of the diesel engines will contribute to the increase of the power and efficiency of the diesel engine, the reduction of both fuel consumption and the emission of greenhouse gases (GHG). This article presents the obtained results from experimental validation of the selected design with an aim to valorize this innovative solution and become trustworthy.
