The non-linear finite element software ABAQUS was used to simulate the dynamic response of a marine supercharged boiler when subjected to impact loading. Shock resistance was analyzed by the time-domain simulation met...The non-linear finite element software ABAQUS was used to simulate the dynamic response of a marine supercharged boiler when subjected to impact loading. Shock resistance was analyzed by the time-domain simulation method. After exhaustive simulations,the effect of air pressure induced by different working conditions on the shock response of a supercharged boiler was reviewed,leading to conclusions about the variability of structural response with different loading parameters. In order to simulate the real impulsive environments of supercharged boilers,the integration of equipment and ship structure was then primarily used to analyze shock response. These distinctly different equipment shock test methods,run under equivalent work conditions,were compared and the causes of discrepancy were analyzed. The main purpose of this paper is to present references for the anti-shock design of marine supercharged boilers.展开更多
DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously dev...DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity.展开更多
文摘The non-linear finite element software ABAQUS was used to simulate the dynamic response of a marine supercharged boiler when subjected to impact loading. Shock resistance was analyzed by the time-domain simulation method. After exhaustive simulations,the effect of air pressure induced by different working conditions on the shock response of a supercharged boiler was reviewed,leading to conclusions about the variability of structural response with different loading parameters. In order to simulate the real impulsive environments of supercharged boilers,the integration of equipment and ship structure was then primarily used to analyze shock response. These distinctly different equipment shock test methods,run under equivalent work conditions,were compared and the causes of discrepancy were analyzed. The main purpose of this paper is to present references for the anti-shock design of marine supercharged boilers.
基金supported by the National Basic Research Program (2011CB911002)the National Natural Science Foundation of China (21190044, 21475037, 21222507, 21175036)the fundamental research funds for the central universities
文摘DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity.
