针对污水处理厂尾水TN去除问题,采用16S r DNA克隆文库法,探究了3DBER-S(三维电极生物膜耦合硫自养脱氮工艺)的强化脱氮机制及其菌群特征.结果表明,I(电流)和HRT(水力停留时间)对3DEBR-S中氢自养和硫自养反硝化作用所占比例的影响较大,...针对污水处理厂尾水TN去除问题,采用16S r DNA克隆文库法,探究了3DBER-S(三维电极生物膜耦合硫自养脱氮工艺)的强化脱氮机制及其菌群特征.结果表明,I(电流)和HRT(水力停留时间)对3DEBR-S中氢自养和硫自养反硝化作用所占比例的影响较大,但对脱氮效率影响不显著.当进水C/N〔ρ(CODCr)/ρ(TN)〕为1、ρ(NO3--N)为35 mg/L、I为300 m A、HRT为4 h时,NO3--N和TN去除率可分别稳定在80%和74%以上.16S r DNA克隆文库结果显示,反应器中β变形菌纲为优势菌群,占47.89%〔以OUT(操作单元)计〕.在β变形菌纲中,与具有反硝化功能的陶厄氏菌属(Thauera)相似的细菌所占比例最大,为52.94%;与可分别利用硫和氢为电子供体进行反硝化脱氮的硫杆菌属(Thiobacillus)和食酸菌属(Acidovorax)相似的细菌分别占17.65%和14.71%.3DBER-S中存在异养联合氢自养和硫自养反硝化协同去除硝酸盐氮的作用,可为反硝化脱氮提供充足的电子供体,节约了有机碳源消耗,并保证了稳定高效的脱氮效果.展开更多
In order to reduce the amount of NO_3^-–N generated by the Anammox process, and alleviate the competition between denitrification and Anammox for NO_2^-–N in a single reactor, the preference of S^0 for reacting with...In order to reduce the amount of NO_3^-–N generated by the Anammox process, and alleviate the competition between denitrification and Anammox for NO_2^-–N in a single reactor, the preference of S^0 for reacting with coexisting NO_2^-–N and NO_3^-–N in the sulfur autotrophic denitrifying(SADN) process and the coupling effect of short-cut SADN and the Anammox process were studied. The results showed that S^0 preferentially reacted with NO_3^-to produce NO_2^-–N, and then reacted with NO_2^-–N when NO_3^-–N was insufficient, which could effectively alleviate the competition between SADN bacteria(SADNB) and Anammox bacteria(An AOB) for NO_2^-–N. After 170 days of operation, coupling between short-cut S^0-SADN and the Anammox process was first successfully achieved. SADNB converted the NO_3^-–N generated by the Anammox process into NO_2^-–N, which was once again available to An AOB. The total nitrogen removal efficiency eventually stabilized at over 95%, and the effluent NO_3^-–N was controlled within 10 mg/L, when high NH_4^+–N wastewater was treated by the Anammox process. Microbial community analysis further showed that Candidatus Brocadia and Thiobacillus were the functional microorganisms for An AOB and SADNB.展开更多
文摘针对污水处理厂尾水TN去除问题,采用16S r DNA克隆文库法,探究了3DBER-S(三维电极生物膜耦合硫自养脱氮工艺)的强化脱氮机制及其菌群特征.结果表明,I(电流)和HRT(水力停留时间)对3DEBR-S中氢自养和硫自养反硝化作用所占比例的影响较大,但对脱氮效率影响不显著.当进水C/N〔ρ(CODCr)/ρ(TN)〕为1、ρ(NO3--N)为35 mg/L、I为300 m A、HRT为4 h时,NO3--N和TN去除率可分别稳定在80%和74%以上.16S r DNA克隆文库结果显示,反应器中β变形菌纲为优势菌群,占47.89%〔以OUT(操作单元)计〕.在β变形菌纲中,与具有反硝化功能的陶厄氏菌属(Thauera)相似的细菌所占比例最大,为52.94%;与可分别利用硫和氢为电子供体进行反硝化脱氮的硫杆菌属(Thiobacillus)和食酸菌属(Acidovorax)相似的细菌分别占17.65%和14.71%.3DBER-S中存在异养联合氢自养和硫自养反硝化协同去除硝酸盐氮的作用,可为反硝化脱氮提供充足的电子供体,节约了有机碳源消耗,并保证了稳定高效的脱氮效果.
基金supported by the National Key Research and Development Programme of China(No.2016YFC 0401103)the National Natural Science Foundation of China(No.51408387)the Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment
文摘In order to reduce the amount of NO_3^-–N generated by the Anammox process, and alleviate the competition between denitrification and Anammox for NO_2^-–N in a single reactor, the preference of S^0 for reacting with coexisting NO_2^-–N and NO_3^-–N in the sulfur autotrophic denitrifying(SADN) process and the coupling effect of short-cut SADN and the Anammox process were studied. The results showed that S^0 preferentially reacted with NO_3^-to produce NO_2^-–N, and then reacted with NO_2^-–N when NO_3^-–N was insufficient, which could effectively alleviate the competition between SADN bacteria(SADNB) and Anammox bacteria(An AOB) for NO_2^-–N. After 170 days of operation, coupling between short-cut S^0-SADN and the Anammox process was first successfully achieved. SADNB converted the NO_3^-–N generated by the Anammox process into NO_2^-–N, which was once again available to An AOB. The total nitrogen removal efficiency eventually stabilized at over 95%, and the effluent NO_3^-–N was controlled within 10 mg/L, when high NH_4^+–N wastewater was treated by the Anammox process. Microbial community analysis further showed that Candidatus Brocadia and Thiobacillus were the functional microorganisms for An AOB and SADNB.
