Quantitative studies on the extension and subsidence of the Wanan Basin were carried out based on available seismic and borehole data together with regional geological data. Using balanced cross-section and backstripp...Quantitative studies on the extension and subsidence of the Wanan Basin were carried out based on available seismic and borehole data together with regional geological data. Using balanced cross-section and backstripping techniques, we reconstructed the stratigraphic deposition and tectonic evolution histories of the basin. The basin formed from the Eocene and was generally in an extensional/transtensional state except for the Late Miocene local compressoin. The major basin extension ocurred in the Oligocene and Early Miocene （before -16.3 Ma） and thereafter uniform stretch in a smaller rate. The northern and middle basin extended intensely earlier during 38.6-23.3 Ma, while the southern basin was mainly stretched during 23.3-16.3 Ma. The basin formation and development are related to alternating sinistral to dextral strike-slip motions along the Wanan Fault Zone. The dominant dynamics may be caused by the seafloor spreading of the South China Sea and the its peripheral plate interaction. The basin tectonic evolution is divided into five phases： initial rifting, main rifting, rift-drift transition, structural inversion, and thermal subsidence.展开更多
A fault-tolerant 1-spanner is used to preserve all the minimum energy paths after node failures to cope with fault-tolerant topology control problems in wireless ad hoc networks. A fault-tolerant 1-spanner is a graph ...A fault-tolerant 1-spanner is used to preserve all the minimum energy paths after node failures to cope with fault-tolerant topology control problems in wireless ad hoc networks. A fault-tolerant 1-spanner is a graph such that the remaining graph after node failures will not only remain connected, but also have a stretch factor of one. The fault-tolerant 1-spanner is used in a localized and distributed topology control algorithm, named the k-Fault-Tolerant 1-Spanner （k-FT1S）, where each node constructs a minimum energy path tree for every local failed node set. This paper proves that the topology constructed by k-FT1S is a k-fault-tolerant 1-spanner that can tolerate up to k node failures, such that the remaining network after node failures preserves all the minimum energy paths of the remaining network gained from the initial network by removing the same failed nodes. Simulations show that the remaining network after removal of any k nodes still has the optimal energy efficiency and is competitive in terms of average logical degree, average physical degree, and average transmission radius.展开更多
Glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS)and overexpression of the EPSPS gene is one of the molecular mechanisms conferring glyphosate resistance in weeds.A regulatory sequence ...Glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS)and overexpression of the EPSPS gene is one of the molecular mechanisms conferring glyphosate resistance in weeds.A regulatory sequence of EPSPS gene was isolated previously,and an alteration in its 5´-untranslated region(UTR)pyrimidine(Py)-rich stretch element is involved in the regulation of EPSPS expression in glyphosate-resistant(GR)Eleusine indica.However,the transcription factors involved in this regulatory sequence remain to be elucidated.In this study,we investigated the regulatory network of EPSPS overexpression associated genes in a GR E.indica population by RNA-seq.The differentially expressed transcript analyses revealed that glyphosate treatment caused an increase in the expression of 2752 unigenes and a decrease in the expression of 4025 unigenes in the GR E.indica,compared to the glyphosatesusceptible(GS)E.indica.Among them,1373 unigenes were identified to be co-expressed with the EPSPS gene in GR E.indica.GO and KEGG pathway analyses showed that the up-regulated unigenes were mainly enriched in chloroplasts and associated with the shikimate biosynthesis pathway,chlorophy II and peroxisome metabolism processes.Notably,the expression of a Shikimate kinase which catalyzed the conversion of Shikimate to Shikimate 3-phosphate(S3P,a substrate of EPSPS),was also up-regulated.Eight transcription factors were identified as likely to be involved in the regulation of the EPSPS expression,and three of them(ARF2,ARF8 and BPC6)showed more binding sites because of a(CT)n insertion of the 5´-UTR Py-rich stretch element in GR.However,the yeast one-hybrid assay illustrated that ARF8 and BPC6 could bind to the 5´-UTR Py-rich stretch element of wild type EPSPS,but could not bind to the mutated form.Our data suggests that the transcriptional regulation of EPSPS expression is complex and was significantly altered in GR E.indica.These discoveries provide new references for further study of the EPSPS overexpression mechanism that endows g展开更多
基金funded by the Fundamental Research Program(No.2009CB219406)of the Chinese Ministry of Sciences and Technologythe Knowledge Innovation Project(No.KZCX2-YW-229)of the Chinese Academy of Sciences
文摘Quantitative studies on the extension and subsidence of the Wanan Basin were carried out based on available seismic and borehole data together with regional geological data. Using balanced cross-section and backstripping techniques, we reconstructed the stratigraphic deposition and tectonic evolution histories of the basin. The basin formed from the Eocene and was generally in an extensional/transtensional state except for the Late Miocene local compressoin. The major basin extension ocurred in the Oligocene and Early Miocene （before -16.3 Ma） and thereafter uniform stretch in a smaller rate. The northern and middle basin extended intensely earlier during 38.6-23.3 Ma, while the southern basin was mainly stretched during 23.3-16.3 Ma. The basin formation and development are related to alternating sinistral to dextral strike-slip motions along the Wanan Fault Zone. The dominant dynamics may be caused by the seafloor spreading of the South China Sea and the its peripheral plate interaction. The basin tectonic evolution is divided into five phases： initial rifting, main rifting, rift-drift transition, structural inversion, and thermal subsidence.
基金Supported by the National Natural Science Foundation of China (No.60932005)
文摘A fault-tolerant 1-spanner is used to preserve all the minimum energy paths after node failures to cope with fault-tolerant topology control problems in wireless ad hoc networks. A fault-tolerant 1-spanner is a graph such that the remaining graph after node failures will not only remain connected, but also have a stretch factor of one. The fault-tolerant 1-spanner is used in a localized and distributed topology control algorithm, named the k-Fault-Tolerant 1-Spanner （k-FT1S）, where each node constructs a minimum energy path tree for every local failed node set. This paper proves that the topology constructed by k-FT1S is a k-fault-tolerant 1-spanner that can tolerate up to k node failures, such that the remaining network after node failures preserves all the minimum energy paths of the remaining network gained from the initial network by removing the same failed nodes. Simulations show that the remaining network after removal of any k nodes still has the optimal energy efficiency and is competitive in terms of average logical degree, average physical degree, and average transmission radius.
基金This study was supported by the National Natural Science Foundation of China(31871984)the Guangdong Provincial National Natural Science Foundation,China(2017B030311006)+1 种基金the Department of Science and Technology of Guangdong Province,China(2019B121201003)the special fund for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science,China(202105TD,R2020PYJX005).
文摘Glyphosate inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS)and overexpression of the EPSPS gene is one of the molecular mechanisms conferring glyphosate resistance in weeds.A regulatory sequence of EPSPS gene was isolated previously,and an alteration in its 5´-untranslated region(UTR)pyrimidine(Py)-rich stretch element is involved in the regulation of EPSPS expression in glyphosate-resistant(GR)Eleusine indica.However,the transcription factors involved in this regulatory sequence remain to be elucidated.In this study,we investigated the regulatory network of EPSPS overexpression associated genes in a GR E.indica population by RNA-seq.The differentially expressed transcript analyses revealed that glyphosate treatment caused an increase in the expression of 2752 unigenes and a decrease in the expression of 4025 unigenes in the GR E.indica,compared to the glyphosatesusceptible(GS)E.indica.Among them,1373 unigenes were identified to be co-expressed with the EPSPS gene in GR E.indica.GO and KEGG pathway analyses showed that the up-regulated unigenes were mainly enriched in chloroplasts and associated with the shikimate biosynthesis pathway,chlorophy II and peroxisome metabolism processes.Notably,the expression of a Shikimate kinase which catalyzed the conversion of Shikimate to Shikimate 3-phosphate(S3P,a substrate of EPSPS),was also up-regulated.Eight transcription factors were identified as likely to be involved in the regulation of the EPSPS expression,and three of them(ARF2,ARF8 and BPC6)showed more binding sites because of a(CT)n insertion of the 5´-UTR Py-rich stretch element in GR.However,the yeast one-hybrid assay illustrated that ARF8 and BPC6 could bind to the 5´-UTR Py-rich stretch element of wild type EPSPS,but could not bind to the mutated form.Our data suggests that the transcriptional regulation of EPSPS expression is complex and was significantly altered in GR E.indica.These discoveries provide new references for further study of the EPSPS overexpression mechanism that endows g
