Transport of vacuolar proteins from Golgi apparatus or trans-Golgi network （TGN） to vacuoles is a receptor-mediated process via an intermediate membrane-bound prevacuolar compartment （PVC） in plant cells. Both vac...Transport of vacuolar proteins from Golgi apparatus or trans-Golgi network （TGN） to vacuoles is a receptor-mediated process via an intermediate membrane-bound prevacuolar compartment （PVC） in plant cells. Both vacuolar sorting receptor （VSR） and receptor homology region-transmembrane domain-RING-H2 （RMR） proteins have been shown to function in transporting storage proteins to protein storage vacuole （PSV）, but little is known about the nature of the PVC for the PSV pathway. Here, we use the rice RMR1 （OsRMR1） as a probe to study the PSV pathway in plants. Immunogold electron microscopy （EM） with specific OsRMR1 antibodies showed that OsRMR1 proteins were found in the Golgi apparatus, TGN, and a distinct organelle with characteristics of PVC in both rice culture cells and developing rice seeds, as well as the protein body type II （PBII） or PSV in developing rice seeds. This organelle, also found in both tobacco BY-2 and Arabidopsis suspension cultured cells, is morphologically distinct from the VSR-positive multivesicular lytic PVC or multivesicular body （MVB） and thus represent a PVC for the PSV pathway that we name storage PVC （sPVC）. Further in vivo and in vitro interaction studies using truncated OsRMR1 proteins secreted into the culture media of transgenic BY-2 suspension cells demonstrated that OsRMR1 functions as a sorting receptor in transporting vicilin-like storage proteins.展开更多
In this paper, the genetic algorithms are applied to the optimization problem of magnet sorting in an electron storage ring, according to which the objectives are set so that the closed orbit distortion and beta beati...In this paper, the genetic algorithms are applied to the optimization problem of magnet sorting in an electron storage ring, according to which the objectives are set so that the closed orbit distortion and beta beating can be minimized and the dynamic aperture maximized. The sorting of dipole, quadrupole and sextupole magnets is optimized while the optimization results show the power of the application of genetic algorithms in magnet sorting.展开更多
Purpose Round beam,i.e.,with equal horizontal and vertical emittance,is preferable than a horizontally flat one for some beamline applications in Diffraction-limited storage rings(DLSRs),for the purposes of reducing t...Purpose Round beam,i.e.,with equal horizontal and vertical emittance,is preferable than a horizontally flat one for some beamline applications in Diffraction-limited storage rings(DLSRs),for the purposes of reducing the number of photons getting discarded and better phase space match between photon and electron beam.Conventional methods of obtaining round beam inescapably results in a reduction of dynamic aperture(DA).In order to recover the DA as much as possible for improving the injection efficiency,the DA optimization by using Non-dominated sorting genetic algorithm-Ⅱ(NSGA-Ⅱ)to generate round beam,particularly to one of the designed lattice of the High Energy Photon Source(HEPS)storage ring,are presented.Method According to the general unconstrained model of NSGA-Ⅱ,we modified the standard model by using parallel computing to optimize round beam lattices with errors,especially for a strong coupling,such as solenoid scheme.Results and conclusion The results of numerical tracking verify the correction of the theory framework of solenoids with fringe fields and demonstrates the feasibility on the HEPS storage ring with errors to operate in round beam mode after optimizing DA.展开更多
In this paper, we research on the research on the mass structured data storage and sorting algorithm and methodology for SQL database under the big data environment. With the data storage market development and center...In this paper, we research on the research on the mass structured data storage and sorting algorithm and methodology for SQL database under the big data environment. With the data storage market development and centering on the server, the data will store model to data- centric data storage model. Storage is considered from the start, just keep a series of data, for the management system and storage device rarely consider the intrinsic value of the stored data. The prosperity of the Internet has changed the world data storage, and with the emergence of many new applications. Theoretically, the proposed algorithm has the ability of dealing with massive data and numerically, the algorithm could enhance the processing accuracy and speed which will be meaningful.展开更多
为解决用最少经济成本使风电场输出功率可最好地满足负荷问题,该文提出以风力机、制-储-燃氢装置的总安装成本、负荷缺电率LPSP(loss of power supply probability)和风氢互补系统输出功率波动率FPP(fluctuation of power probability)...为解决用最少经济成本使风电场输出功率可最好地满足负荷问题,该文提出以风力机、制-储-燃氢装置的总安装成本、负荷缺电率LPSP(loss of power supply probability)和风氢互补系统输出功率波动率FPP(fluctuation of power probability)为指标的容量配置方案。利用某地区一天的风速及负荷,在Matlab中编写基于精英非支配排序遗传算法NSGA-Ⅱ(elitist non-dominated sorting genetic algorithm)的多目标函数优化程序,对风电机组、电解槽-储氢罐-燃氢燃气轮机系统进行最优容量配置,得出优化组合方案。优化后的互补系统可有效保证其经济性和供电可靠性。展开更多
文摘Transport of vacuolar proteins from Golgi apparatus or trans-Golgi network （TGN） to vacuoles is a receptor-mediated process via an intermediate membrane-bound prevacuolar compartment （PVC） in plant cells. Both vacuolar sorting receptor （VSR） and receptor homology region-transmembrane domain-RING-H2 （RMR） proteins have been shown to function in transporting storage proteins to protein storage vacuole （PSV）, but little is known about the nature of the PVC for the PSV pathway. Here, we use the rice RMR1 （OsRMR1） as a probe to study the PSV pathway in plants. Immunogold electron microscopy （EM） with specific OsRMR1 antibodies showed that OsRMR1 proteins were found in the Golgi apparatus, TGN, and a distinct organelle with characteristics of PVC in both rice culture cells and developing rice seeds, as well as the protein body type II （PBII） or PSV in developing rice seeds. This organelle, also found in both tobacco BY-2 and Arabidopsis suspension cultured cells, is morphologically distinct from the VSR-positive multivesicular lytic PVC or multivesicular body （MVB） and thus represent a PVC for the PSV pathway that we name storage PVC （sPVC）. Further in vivo and in vitro interaction studies using truncated OsRMR1 proteins secreted into the culture media of transgenic BY-2 suspension cells demonstrated that OsRMR1 functions as a sorting receptor in transporting vicilin-like storage proteins.
基金Supported by National Natural Science Foundation of China(10979045,11175180,11175182)
文摘In this paper, the genetic algorithms are applied to the optimization problem of magnet sorting in an electron storage ring, according to which the objectives are set so that the closed orbit distortion and beta beating can be minimized and the dynamic aperture maximized. The sorting of dipole, quadrupole and sextupole magnets is optimized while the optimization results show the power of the application of genetic algorithms in magnet sorting.
基金supported by the Guangdong Joint fund for basic and applied basic research(Guangdong-dongguan,Grant Number 2019B1515120069,2019).
文摘Purpose Round beam,i.e.,with equal horizontal and vertical emittance,is preferable than a horizontally flat one for some beamline applications in Diffraction-limited storage rings(DLSRs),for the purposes of reducing the number of photons getting discarded and better phase space match between photon and electron beam.Conventional methods of obtaining round beam inescapably results in a reduction of dynamic aperture(DA).In order to recover the DA as much as possible for improving the injection efficiency,the DA optimization by using Non-dominated sorting genetic algorithm-Ⅱ(NSGA-Ⅱ)to generate round beam,particularly to one of the designed lattice of the High Energy Photon Source(HEPS)storage ring,are presented.Method According to the general unconstrained model of NSGA-Ⅱ,we modified the standard model by using parallel computing to optimize round beam lattices with errors,especially for a strong coupling,such as solenoid scheme.Results and conclusion The results of numerical tracking verify the correction of the theory framework of solenoids with fringe fields and demonstrates the feasibility on the HEPS storage ring with errors to operate in round beam mode after optimizing DA.
文摘In this paper, we research on the research on the mass structured data storage and sorting algorithm and methodology for SQL database under the big data environment. With the data storage market development and centering on the server, the data will store model to data- centric data storage model. Storage is considered from the start, just keep a series of data, for the management system and storage device rarely consider the intrinsic value of the stored data. The prosperity of the Internet has changed the world data storage, and with the emergence of many new applications. Theoretically, the proposed algorithm has the ability of dealing with massive data and numerically, the algorithm could enhance the processing accuracy and speed which will be meaningful.
文摘为解决用最少经济成本使风电场输出功率可最好地满足负荷问题,该文提出以风力机、制-储-燃氢装置的总安装成本、负荷缺电率LPSP(loss of power supply probability)和风氢互补系统输出功率波动率FPP(fluctuation of power probability)为指标的容量配置方案。利用某地区一天的风速及负荷,在Matlab中编写基于精英非支配排序遗传算法NSGA-Ⅱ(elitist non-dominated sorting genetic algorithm)的多目标函数优化程序,对风电机组、电解槽-储氢罐-燃氢燃气轮机系统进行最优容量配置,得出优化组合方案。优化后的互补系统可有效保证其经济性和供电可靠性。
