Background:Traditional Chinese medicine requires special processing before it can be effectively used for clinical treatment of diseases.In particular,Tangshen stir-fried with wheat bran smoke has been found to enhanc...Background:Traditional Chinese medicine requires special processing before it can be effectively used for clinical treatment of diseases.In particular,Tangshen stir-fried with wheat bran smoke has been found to enhance the function of the stomach and spleen.However,there is limited research on the technology and pharmacodynamic effects of Tangshen fried with bran.Therefore,the objective of this article is to optimize the method of stir-frying with bran for Tangshen processing and to compare the anti-fatigue and hypoxia-resistance effects of Tangshen before and after bran-frying.Methods:The response surface methodology was utilized to optimize the frying temperature,frying time,and the bran-to-Tangshen ratio,using lobetyolin as the indicator.The swimming time of mice and the levels of blood urea nitrogen,lactate dehydrogenase,and blood glucose in fatigued mice were compared before and after Tangshen was fried with bran.Additionally,the hypoxic resistance ability of Tangshen before and after bran-frying was determined through normobaric hypoxia test and sodium nitrite poisoning test.Results:The optimal bran-frying conditions for Tangshen processing were determined as:frying temperature of 167°C,frying time of 2.67 min,and the bran-to-Tangshen ratio of 50:100.Pharmacodynamic analysis results indicated that Tangshen significantly increased the GLU content(P<0.01)and significantly reduced the LDH content(P<0.01)both before and after bran-frying.While both traditional and new bran-frying methods significantly increased the content of muscle glycogen(P<0.05),the optimized method in this study also significantly elevated the content of liver glycogen(P<0.05).The traditional method significantly prolonged the survival time of mice from sodium nitrite poisoning(P<0.05).Conclusion:The response surface model proved to be highly effective in optimizing the stir-frying process with bran for Tangshen processing.The results of this study indicate that Tangshen possesses anti-fatigue and hypoxia-resistance effects both before an展开更多
目的优选巴戟天酒炙工艺,并比较炮制前后对环磷酰胺所致生精障碍雄性小鼠生殖氧化应激的影响。方法采用L16(4^(5))正交试验设计,以蔗糖、1-蔗果三糖、耐斯糖、1F-果呋喃糖基耐斯糖4种寡糖总含量为评价指标,结合方差分析和熵权逼近理想...目的优选巴戟天酒炙工艺,并比较炮制前后对环磷酰胺所致生精障碍雄性小鼠生殖氧化应激的影响。方法采用L16(4^(5))正交试验设计,以蔗糖、1-蔗果三糖、耐斯糖、1F-果呋喃糖基耐斯糖4种寡糖总含量为评价指标,结合方差分析和熵权逼近理想解排序法(technique for order of preference by similarity to ideal solution,TOPSIS)筛选酒炙巴戟天的工艺参数,并比较巴戟天酒炙前后对环磷酰胺所致生精障碍雄性小鼠生殖氧化应激的影响。结果酒炙巴戟天最佳炮制工艺参数:100 g饮片加黄酒20 mL,密闭闷润2 h,炒制温度60℃,炒制时间7 min,于50℃干燥1 h。药效实验结果表明,巴戟天酒炙后总寡糖对模型小鼠生殖氧化应激作用明显优于生品总寡糖(P<0.05)。结论首次运用熵权TOPSIS法优选巴戟天酒炙工艺,结果简便、准确、可行;药效实验结果为巴戟天酒炙的合理性提供科学依据。展开更多
基金This work was supported by the Key Project of the Key R&D Program of the Department of Science and Technology of Shanxi Province(201603D3112002).
文摘Background:Traditional Chinese medicine requires special processing before it can be effectively used for clinical treatment of diseases.In particular,Tangshen stir-fried with wheat bran smoke has been found to enhance the function of the stomach and spleen.However,there is limited research on the technology and pharmacodynamic effects of Tangshen fried with bran.Therefore,the objective of this article is to optimize the method of stir-frying with bran for Tangshen processing and to compare the anti-fatigue and hypoxia-resistance effects of Tangshen before and after bran-frying.Methods:The response surface methodology was utilized to optimize the frying temperature,frying time,and the bran-to-Tangshen ratio,using lobetyolin as the indicator.The swimming time of mice and the levels of blood urea nitrogen,lactate dehydrogenase,and blood glucose in fatigued mice were compared before and after Tangshen was fried with bran.Additionally,the hypoxic resistance ability of Tangshen before and after bran-frying was determined through normobaric hypoxia test and sodium nitrite poisoning test.Results:The optimal bran-frying conditions for Tangshen processing were determined as:frying temperature of 167°C,frying time of 2.67 min,and the bran-to-Tangshen ratio of 50:100.Pharmacodynamic analysis results indicated that Tangshen significantly increased the GLU content(P<0.01)and significantly reduced the LDH content(P<0.01)both before and after bran-frying.While both traditional and new bran-frying methods significantly increased the content of muscle glycogen(P<0.05),the optimized method in this study also significantly elevated the content of liver glycogen(P<0.05).The traditional method significantly prolonged the survival time of mice from sodium nitrite poisoning(P<0.05).Conclusion:The response surface model proved to be highly effective in optimizing the stir-frying process with bran for Tangshen processing.The results of this study indicate that Tangshen possesses anti-fatigue and hypoxia-resistance effects both before an
文摘目的优选巴戟天酒炙工艺,并比较炮制前后对环磷酰胺所致生精障碍雄性小鼠生殖氧化应激的影响。方法采用L16(4^(5))正交试验设计,以蔗糖、1-蔗果三糖、耐斯糖、1F-果呋喃糖基耐斯糖4种寡糖总含量为评价指标,结合方差分析和熵权逼近理想解排序法(technique for order of preference by similarity to ideal solution,TOPSIS)筛选酒炙巴戟天的工艺参数,并比较巴戟天酒炙前后对环磷酰胺所致生精障碍雄性小鼠生殖氧化应激的影响。结果酒炙巴戟天最佳炮制工艺参数:100 g饮片加黄酒20 mL,密闭闷润2 h,炒制温度60℃,炒制时间7 min,于50℃干燥1 h。药效实验结果表明,巴戟天酒炙后总寡糖对模型小鼠生殖氧化应激作用明显优于生品总寡糖(P<0.05)。结论首次运用熵权TOPSIS法优选巴戟天酒炙工艺,结果简便、准确、可行;药效实验结果为巴戟天酒炙的合理性提供科学依据。
