Neuropathic pain often occurs during chemotherapy with oxaliplatin. AG490 has been shown to exert an antagonistic effect on inflammatory pain, but its effect on oxaliplatin-induced neuropathic pain remains poorly unde...Neuropathic pain often occurs during chemotherapy with oxaliplatin. AG490 has been shown to exert an antagonistic effect on inflammatory pain, but its effect on oxaliplatin-induced neuropathic pain remains poorly understood. This study sought to observe the analgesic effect of AG490 on acute neuropathic pain induced by a single oxaliplatin treatment and to address the possible mechanism. In this study, we estab- lished a model of oxaliplatin-induced acute neuropathic pain by intraperitoneal injection of 6 mg/kg oxaliplatin. On day 2 after injection, models were intraperitoneally injected with i, 5, or 10 mg/kg AG490. Paw withdrawal threshold to mechanical stimuli and tail withdrawal latency to cold stimuli were determined. Western blot assay was performed to detect the expression of spinal phosphorylated signal transducer and activator of transcription 3 （p-STAT3）. Immunohistochemistry was used to determine the immunoreactivity of p-STAT3 and inter- leukin-6. Results demonstrated that paw withdrawal threshold and tail withdrawal latency were significantly increased by the treatment of AG490 in rats. There was no significant difference in the effect among the different doses of AG490. AG490 10 mg/kg decreased the expression of p-STAT3, the immunoreactivity of p-STAT3 and interleukin-6 in spinal cord of acute neuropathic pain rats. These findings confirm that AG490 can attenuate oxaliplatin-induced acute neuropathic pain and is associated with the inhibition in the JAK/STAT3 signaling pathway.展开更多
微地震事件初至的精确拾取是微震时空定位的关键技术之一。简述了STA/LTA(Short–Term to Long–Term Average)、AIC(Akaike Information Criteria)、分形维数3种微地震初至拾取方法的基本原理;采用理论模型数据对不同初至拾取方法进行...微地震事件初至的精确拾取是微震时空定位的关键技术之一。简述了STA/LTA(Short–Term to Long–Term Average)、AIC(Akaike Information Criteria)、分形维数3种微地震初至拾取方法的基本原理;采用理论模型数据对不同初至拾取方法进行了方法测试效果分析;并选取不同信噪比的实际数据对初至拾取精度、算法效率两个方面进行了比较。结果显示:高信噪比时,3种方法初至拾取的精度都比较高;在信噪比降低时,分形维数法初至拾取的精度仍然较高,具有较好的抗噪性;但是,分形维数法的效率较低,且受算法原理限制,并且与AIC法很难单独拾取事件初至。因此,采用STA/LTA识别微地震事件,初步确定初至范围,然后再使用AIC方法精确拾取初至,是微地震事件初至拾取的较好方法。展开更多
基金supported by the National Natural Science Foundation of China,No.81671962
文摘Neuropathic pain often occurs during chemotherapy with oxaliplatin. AG490 has been shown to exert an antagonistic effect on inflammatory pain, but its effect on oxaliplatin-induced neuropathic pain remains poorly understood. This study sought to observe the analgesic effect of AG490 on acute neuropathic pain induced by a single oxaliplatin treatment and to address the possible mechanism. In this study, we estab- lished a model of oxaliplatin-induced acute neuropathic pain by intraperitoneal injection of 6 mg/kg oxaliplatin. On day 2 after injection, models were intraperitoneally injected with i, 5, or 10 mg/kg AG490. Paw withdrawal threshold to mechanical stimuli and tail withdrawal latency to cold stimuli were determined. Western blot assay was performed to detect the expression of spinal phosphorylated signal transducer and activator of transcription 3 （p-STAT3）. Immunohistochemistry was used to determine the immunoreactivity of p-STAT3 and inter- leukin-6. Results demonstrated that paw withdrawal threshold and tail withdrawal latency were significantly increased by the treatment of AG490 in rats. There was no significant difference in the effect among the different doses of AG490. AG490 10 mg/kg decreased the expression of p-STAT3, the immunoreactivity of p-STAT3 and interleukin-6 in spinal cord of acute neuropathic pain rats. These findings confirm that AG490 can attenuate oxaliplatin-induced acute neuropathic pain and is associated with the inhibition in the JAK/STAT3 signaling pathway.
文摘微地震事件初至的精确拾取是微震时空定位的关键技术之一。简述了STA/LTA(Short–Term to Long–Term Average)、AIC(Akaike Information Criteria)、分形维数3种微地震初至拾取方法的基本原理;采用理论模型数据对不同初至拾取方法进行了方法测试效果分析;并选取不同信噪比的实际数据对初至拾取精度、算法效率两个方面进行了比较。结果显示:高信噪比时,3种方法初至拾取的精度都比较高;在信噪比降低时,分形维数法初至拾取的精度仍然较高,具有较好的抗噪性;但是,分形维数法的效率较低,且受算法原理限制,并且与AIC法很难单独拾取事件初至。因此,采用STA/LTA识别微地震事件,初步确定初至范围,然后再使用AIC方法精确拾取初至,是微地震事件初至拾取的较好方法。
