建立了水产品中多组分生物胺的反相高效液相色谱-柱后衍生-荧光检测方法。采用荧光试剂邻苯二甲醛(OPA)为柱后衍生化试剂,在Capcell Pak MG-C18色谱柱上,通过梯度洗脱使酪胺、腐胺、尸胺、组胺、胍丁胺、精胺和亚精胺等7种生物胺得到良...建立了水产品中多组分生物胺的反相高效液相色谱-柱后衍生-荧光检测方法。采用荧光试剂邻苯二甲醛(OPA)为柱后衍生化试剂,在Capcell Pak MG-C18色谱柱上,通过梯度洗脱使酪胺、腐胺、尸胺、组胺、胍丁胺、精胺和亚精胺等7种生物胺得到良好分离,在给定的浓度范围内,各组分生物胺呈现良好线性相关(R2>0.999)。在水产品中添加生物胺混合标准溶液,平均回收率为88.3%～110.1%,相对标准偏差RSD小于10%。结合水产品的感官鉴定、pH值和TVBN值测定等方法检测水产食品的新鲜程度,分析了鱿鱼在不同保藏温度、保藏时间下的生物胺种类及含量的变化。其中胍丁胺和尸胺在鱿鱼的保藏过程中发生最显著变化,可以作为其质量变化的参考指标。展开更多
The undulating fin propulsion system is an instance of the bio-inspired propulsion systems. In the current study, the swimming motion of a squid-like robot with two undulating side fins, mimicking those of a Stingray ...The undulating fin propulsion system is an instance of the bio-inspired propulsion systems. In the current study, the swimming motion of a squid-like robot with two undulating side fins, mimicking those of a Stingray or a Cuttlefish, was investigated through flow computation around the body. We used the finite analytic method for space discretization and Euler implicit scheme for time discretization along with the PISO algorithm for velocity pressure coupling. A body-fitted moving grid was generated using the Poisson equation at each time step. Based on the computed results, we discussed the features of the flow field and hydrodynamic forces acting on the body and fin. A simple relationship among the fin＇s principal dimensions was established. Numerical computation was done for various aspect ratios, fin angles and frequencies in order to validate the proposed relationship among principal dimensions. Subsequently, the relationship was examined base on the distribution of pressure difference between upper and lower surfaces and the distribution of the thrust force. In efficiency calculations, the undulating fins showed promising results. Finally, for the fin, the open characteristics from computed data showed satisfactory conformity with the experimental results.展开更多
文摘建立了水产品中多组分生物胺的反相高效液相色谱-柱后衍生-荧光检测方法。采用荧光试剂邻苯二甲醛(OPA)为柱后衍生化试剂,在Capcell Pak MG-C18色谱柱上,通过梯度洗脱使酪胺、腐胺、尸胺、组胺、胍丁胺、精胺和亚精胺等7种生物胺得到良好分离,在给定的浓度范围内,各组分生物胺呈现良好线性相关(R2>0.999)。在水产品中添加生物胺混合标准溶液,平均回收率为88.3%～110.1%,相对标准偏差RSD小于10%。结合水产品的感官鉴定、pH值和TVBN值测定等方法检测水产食品的新鲜程度,分析了鱿鱼在不同保藏温度、保藏时间下的生物胺种类及含量的变化。其中胍丁胺和尸胺在鱿鱼的保藏过程中发生最显著变化,可以作为其质量变化的参考指标。
文摘The undulating fin propulsion system is an instance of the bio-inspired propulsion systems. In the current study, the swimming motion of a squid-like robot with two undulating side fins, mimicking those of a Stingray or a Cuttlefish, was investigated through flow computation around the body. We used the finite analytic method for space discretization and Euler implicit scheme for time discretization along with the PISO algorithm for velocity pressure coupling. A body-fitted moving grid was generated using the Poisson equation at each time step. Based on the computed results, we discussed the features of the flow field and hydrodynamic forces acting on the body and fin. A simple relationship among the fin＇s principal dimensions was established. Numerical computation was done for various aspect ratios, fin angles and frequencies in order to validate the proposed relationship among principal dimensions. Subsequently, the relationship was examined base on the distribution of pressure difference between upper and lower surfaces and the distribution of the thrust force. In efficiency calculations, the undulating fins showed promising results. Finally, for the fin, the open characteristics from computed data showed satisfactory conformity with the experimental results.
