Trichomerium is a genus of foliar epiphytes with the appearance of sooty moulds,mostly occurring on the surface of living leaves and apparently gaining their nutrients from insect exudates.Species have ascostromata wi...Trichomerium is a genus of foliar epiphytes with the appearance of sooty moulds,mostly occurring on the surface of living leaves and apparently gaining their nutrients from insect exudates.Species have ascostromata with setae and develop on a loosely interwoven mycelial mass of dark brown hyphae,while asci have a bitunicate appearance with hyaline ascospores.In this study,we made 16 collections of Trichomerium from Thailand.All were isolated,and the LSU and ITS rDNA gene regions sequenced.Phylogenetic analysis indicated that the Trichomerium species form a monophyletic clade within Chaetothyriales and warrant the introduction of a new family Trichomeriaceae.Bootstrap support for the Chaetothyriales is 100%and clearly separates Trichomeriaceae from Capnodiales which are morphologically very similar.A detailed account of Trichomerium is provided and we describe and illustrate three new species based on morphological and molecular data.We propose that T.foliicola is adopted as the generic type of Trichomerium because it has been impossible to obtain the holotype specimen of T.coffeicola and also no molecular data exists in worldwide databases for this species or genus.展开更多
An evaluation of the incidence of Crypticerya multicicatrices and Maconellicoccus hirsutus in Colombian Seaflower Biosphere Reserve in San Andrés, Providence and Santa Catalina islands, from mid-August to mid-Nov...An evaluation of the incidence of Crypticerya multicicatrices and Maconellicoccus hirsutus in Colombian Seaflower Biosphere Reserve in San Andrés, Providence and Santa Catalina islands, from mid-August to mid-November 2012 was made. 38 locations were sampled, and 34% of sites tested show an incidence of C. multicicatrices oscillating between a range of 80%-100%, 24% with 40%-80% of incidence, 18% between 10%-40% and 24% with a range of 0%-10%. The occurrence of M. hirsutus fluctuated between 0.5% and 17%. There were seven genera of ants associated C. multicicatrices and 94 hosts of this agriculturally important insect and we found high incidence of associated sooty mold in C. multicicatrices hosts. Observations also contained some developmental stages of C. multicatrices.展开更多
In this paper,a hybrid model based on sooty tern optimization algo-rithm(STOA)is proposed to optimize the parameters of the support vector machine(SVM)and identify the best feature sets simultaneously.Feature selec-ti...In this paper,a hybrid model based on sooty tern optimization algo-rithm(STOA)is proposed to optimize the parameters of the support vector machine(SVM)and identify the best feature sets simultaneously.Feature selec-tion is an essential process of data preprocessing,and it aims to find the most rele-vant subset of features.In recent years,it has been applied in many practical domains of intelligent systems.The application of SVM in many fields has proved its effectiveness in classification tasks of various types.Its performance is mainly determined by the kernel type and its parameters.One of the most challenging process in machine learning is feature selection,intending to select effective and representative features.The main disadvantages of feature selection processes included in classical optimization algorithm are local optimal stagnation and slow convergence.Therefore,the hybrid model proposed in this paper merges the STOA and differential evolution(DE)to improve the search efficiency and con-vergence rate.A series of experiments are conducted on 12 datasets from the UCI repository to comprehensively and objectively evaluate the performance of the proposed method.The superiority of the proposed method is illustrated from dif-ferent aspects,such as the classification accuracy,convergence performance,reduced feature dimensionality,standard deviation(STD),and computation time.展开更多
Capnodium berberidis,a sooty mould fungus,previously reported only from Pakistan in 1978,was recently observed on Berberis lycium in Jot Pass,district Chamba of Himachal Pradesh,India.Berberis lycium is a well-known s...Capnodium berberidis,a sooty mould fungus,previously reported only from Pakistan in 1978,was recently observed on Berberis lycium in Jot Pass,district Chamba of Himachal Pradesh,India.Berberis lycium is a well-known small to medium sized spiny,woody,deciduous or evergreen medicinal and ornamental plant distributed throughout temperate and subtropical regions of the world.Disease symptoms appeared as thin,black to dark brown,easily removed superficial layer of hyphae on various parts of host plant including leaves,stem,twigs and fruits.The detailed studies on its morphology and taxonomy revealed it a sooty mould fungus Capnodium berberidis.As per literature consulted,this is the first report of Capnodium berberidis from India and probably second from world.展开更多
本研究旨在比较CTAB法、改良CTAB法、SDS法、氯化苄法和Chelex-100法提取真菌DNA作为PCR模板的效果,以生长速率、菌落形态差异较大的11个煤污病菌属真菌为研究对象,以核糖体RNA(r RNA)基因中的内转录间隔区(ITS)序列和28S r RNA基因部...本研究旨在比较CTAB法、改良CTAB法、SDS法、氯化苄法和Chelex-100法提取真菌DNA作为PCR模板的效果,以生长速率、菌落形态差异较大的11个煤污病菌属真菌为研究对象,以核糖体RNA(r RNA)基因中的内转录间隔区(ITS)序列和28S r RNA基因部分序列的扩增率为指标,采用SPSS软件对结果进行方差分析。结果表明,CTAB法和改良CTAB法适用真菌范围最广,分别有7个和9个属真菌的扩增率都大于70%且无显著性差异;氯化苄法和Chelex-100法适用范围居中,分别有6个和8个属真菌的扩增率较高且无显著性差异,扩增率分别大于70%和50%;SDS法适用范围最窄,有6个属真菌的扩增率高于50%且无显著性差异。大多数煤污病菌至少有2种及以上的DNA提取方法能够取得较好的效果,但链丝孢属真菌只有用氯化苄法提取DNA效果最好。采用改良CTAB法结合氯化苄法,能够满足所有供试煤污病菌类群真菌提取基因组DNA用作PCR反应模板的需要。展开更多
基金supported by the Thailand Research Fund BRG528002.
文摘Trichomerium is a genus of foliar epiphytes with the appearance of sooty moulds,mostly occurring on the surface of living leaves and apparently gaining their nutrients from insect exudates.Species have ascostromata with setae and develop on a loosely interwoven mycelial mass of dark brown hyphae,while asci have a bitunicate appearance with hyaline ascospores.In this study,we made 16 collections of Trichomerium from Thailand.All were isolated,and the LSU and ITS rDNA gene regions sequenced.Phylogenetic analysis indicated that the Trichomerium species form a monophyletic clade within Chaetothyriales and warrant the introduction of a new family Trichomeriaceae.Bootstrap support for the Chaetothyriales is 100%and clearly separates Trichomeriaceae from Capnodiales which are morphologically very similar.A detailed account of Trichomerium is provided and we describe and illustrate three new species based on morphological and molecular data.We propose that T.foliicola is adopted as the generic type of Trichomerium because it has been impossible to obtain the holotype specimen of T.coffeicola and also no molecular data exists in worldwide databases for this species or genus.
文摘An evaluation of the incidence of Crypticerya multicicatrices and Maconellicoccus hirsutus in Colombian Seaflower Biosphere Reserve in San Andrés, Providence and Santa Catalina islands, from mid-August to mid-November 2012 was made. 38 locations were sampled, and 34% of sites tested show an incidence of C. multicicatrices oscillating between a range of 80%-100%, 24% with 40%-80% of incidence, 18% between 10%-40% and 24% with a range of 0%-10%. The occurrence of M. hirsutus fluctuated between 0.5% and 17%. There were seven genera of ants associated C. multicicatrices and 94 hosts of this agriculturally important insect and we found high incidence of associated sooty mold in C. multicicatrices hosts. Observations also contained some developmental stages of C. multicatrices.
基金Sanming University introduces high-level talents to start scientific research funding support project(20YG14,20YG01)Guiding science and technology projects in Sanming City(2020-G-61,2020-S-39)+1 种基金Educational research projects of young and middle-aged teachers in Fujian Province(JAT200618,JAT200638)Scientific research and development fund of Sanming University(B202009,B202029).
文摘In this paper,a hybrid model based on sooty tern optimization algo-rithm(STOA)is proposed to optimize the parameters of the support vector machine(SVM)and identify the best feature sets simultaneously.Feature selec-tion is an essential process of data preprocessing,and it aims to find the most rele-vant subset of features.In recent years,it has been applied in many practical domains of intelligent systems.The application of SVM in many fields has proved its effectiveness in classification tasks of various types.Its performance is mainly determined by the kernel type and its parameters.One of the most challenging process in machine learning is feature selection,intending to select effective and representative features.The main disadvantages of feature selection processes included in classical optimization algorithm are local optimal stagnation and slow convergence.Therefore,the hybrid model proposed in this paper merges the STOA and differential evolution(DE)to improve the search efficiency and con-vergence rate.A series of experiments are conducted on 12 datasets from the UCI repository to comprehensively and objectively evaluate the performance of the proposed method.The superiority of the proposed method is illustrated from dif-ferent aspects,such as the classification accuracy,convergence performance,reduced feature dimensionality,standard deviation(STD),and computation time.
文摘Capnodium berberidis,a sooty mould fungus,previously reported only from Pakistan in 1978,was recently observed on Berberis lycium in Jot Pass,district Chamba of Himachal Pradesh,India.Berberis lycium is a well-known small to medium sized spiny,woody,deciduous or evergreen medicinal and ornamental plant distributed throughout temperate and subtropical regions of the world.Disease symptoms appeared as thin,black to dark brown,easily removed superficial layer of hyphae on various parts of host plant including leaves,stem,twigs and fruits.The detailed studies on its morphology and taxonomy revealed it a sooty mould fungus Capnodium berberidis.As per literature consulted,this is the first report of Capnodium berberidis from India and probably second from world.
文摘本研究旨在比较CTAB法、改良CTAB法、SDS法、氯化苄法和Chelex-100法提取真菌DNA作为PCR模板的效果,以生长速率、菌落形态差异较大的11个煤污病菌属真菌为研究对象,以核糖体RNA(r RNA)基因中的内转录间隔区(ITS)序列和28S r RNA基因部分序列的扩增率为指标,采用SPSS软件对结果进行方差分析。结果表明,CTAB法和改良CTAB法适用真菌范围最广,分别有7个和9个属真菌的扩增率都大于70%且无显著性差异;氯化苄法和Chelex-100法适用范围居中,分别有6个和8个属真菌的扩增率较高且无显著性差异,扩增率分别大于70%和50%;SDS法适用范围最窄,有6个属真菌的扩增率高于50%且无显著性差异。大多数煤污病菌至少有2种及以上的DNA提取方法能够取得较好的效果,但链丝孢属真菌只有用氯化苄法提取DNA效果最好。采用改良CTAB法结合氯化苄法,能够满足所有供试煤污病菌类群真菌提取基因组DNA用作PCR反应模板的需要。
