目的观察石斛水溶性多糖(solub le polysaccharides fromDendrobium candidum,SPD)对在体肿瘤和离体培养肿瘤细胞的抑制作用,探讨其抑制作用的可能机制。方法观察SPD对小鼠接种肉瘤(S180)瘤体生长,离体培养人肝肿瘤细胞(SMMC27721)生长...目的观察石斛水溶性多糖(solub le polysaccharides fromDendrobium candidum,SPD)对在体肿瘤和离体培养肿瘤细胞的抑制作用,探讨其抑制作用的可能机制。方法观察SPD对小鼠接种肉瘤(S180)瘤体生长,离体培养人肝肿瘤细胞(SMMC27721)生长的抑制作用;分析小鼠外周血中超氧物歧化酶活性(superoxide d ismutase,SOD)和膜脂过氧化产物丙二醛(m alond ialdehyde,MDA)含量,小鼠胸腺指数和脾脏指数的变化;核染色(Hoechst染色)法观察SPD对人神经母瘤细胞(SH-SY5Y)细胞凋亡的影响。结果SPD处理能够有效抑制小鼠肉瘤(S180)瘤体生长和离体肝肿瘤细胞生长(P<0.05);有效提高荷瘤小鼠胸腺指数和脾脏指数,提高SOD活性和降低MDA含量(P<0.05);促进神经母瘤细胞凋亡。结论SPD具有显著的抗肿瘤效应,其抑瘤机制可能与SPD促进免疫器官功能和提高抗氧化能力有关。展开更多
The present study characterised the types and amounts of non-starch polysaccharides(NSP)remaining undigested along the gastrointestinal tract(GIT)of broiler chickens offered a typical wheat-or maizebased diet.One-day ...The present study characterised the types and amounts of non-starch polysaccharides(NSP)remaining undigested along the gastrointestinal tract(GIT)of broiler chickens offered a typical wheat-or maizebased diet.One-day old Cobb 500 mixed-sex chicks were assigned to 24 pens,with 10 birds/pen and 12 pens/treatment.Birds were offered the experimental diets in 3 phases(starter,day 0 to 10;grower,day 11 to 24 and finisher,day 25 to 35).Excreta and digesta samples from the crop,gizzard,duodenum,jejunum,ileum and caeca were collected at 12 and 35 days of age,and analysed for the NSP flow.The wheat-based diet contained higher levels of soluble NSP than the maize-based diet,whereas insoluble NSP levels were similar between the 2 diets.Detailed analysis of NSP constituents revealed that arabinoxylans were the primary NSP in the wheat-based diet,mostly in insoluble form.Pectins were the predominant NSP in the maize-based diet,followed by arabinoxylans.Overall,birds offered the wheatbased diet presented higher levels of soluble NSP remaining in all gut sections compared to birds offered the maize-based diet,at both 12 and 35 days of age(P<0.050).Accumulation of insoluble NSP in the gizzard was noted in birds fed both diets,but was more pronounced in birds offered the maize-based diet compared to the wheat-based diet,at both 12 and 35 days of age(P<0.001).The present study highlights marked differences in the amounts and types of NSP delivered to the different gut sections when feeding wheat-compared to maize-based diets,particularly in the gizzard and the lower GIT of birds.展开更多
Dendrobium officinale has high medicinal value but grows slowly in natural environment due to its special CAM photosynthetic pathway.In this study,D.officinale were grown aeroponically with light/dark cycles of 12 h/1...Dendrobium officinale has high medicinal value but grows slowly in natural environment due to its special CAM photosynthetic pathway.In this study,D.officinale were grown aeroponically with light/dark cycles of 12 h/12 h,4 h/4 h,and 2 h/2 h for 150 d.The photosynthetic electron transfer characteristics,photosynthetic CO_(2) fixation pathways,and accumulations of biomass and soluble polysaccharides in D.officinale leaves were studied.The results showed that the photosynthetic apparatus states of D.officinale in aeroponic cultivation under short light/dark cycles of 4 h/4 h and 2 h/2 h were better than that under 12 h/12 h.The dark net CO_(2) exchange percentages of D.officinale were negative in short light/dark cycles of 4 h/4 h and 2 h/2 h,and the daily net CO_(2) exchange amount and dry/fresh weight increases were doubled compared with those in 12 h/12 h light/dark cycle.High soluble polysaccharides content and the soluble polysaccharides yield of D.officinale were obtained in the shorter light/dark cycle of 2 h/2 h.Therefore,the photosynthetic pathway of D.officinale could be switched from CAM to C3 by short light/dark cycles of 4 h/4 h and 2 h/2 h,and its higher biomass accumulation and soluble polysaccharides yield could be obtained by the shorter light/dark cycle of 2 h/2 h in aeroponic cultivation.展开更多
目的优选菟丝子多糖提取的最佳工艺并探讨菟丝子多糖在应激状态下对小鼠脑组织脂质过氧化物反应的影响。方法采用正交实验设计方法优选最佳工艺;比色法测定小鼠脑组织超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果影响菟丝子多糖提取...目的优选菟丝子多糖提取的最佳工艺并探讨菟丝子多糖在应激状态下对小鼠脑组织脂质过氧化物反应的影响。方法采用正交实验设计方法优选最佳工艺;比色法测定小鼠脑组织超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果影响菟丝子多糖提取率的主要因素为提取温度与料液比;不同剂量的菟丝子多糖均能降低MDA的含量(P<0.05),并能增加SOD的活性(P<0.05)。结论菟丝子多糖最佳提取工艺为超声提取,料液比为1∶15,提取温度为80℃,提取时间为60 m in;菟丝子多糖具有抗脂质过氧化保护脑组织的作用。展开更多
The incidence rate of colon cancer ranks the third among malignant tumors worldwide.Gloeostereum incarnatum(GI),a fungus with homology of medicine and food,has multiple pharmacological activities.It was investigated i...The incidence rate of colon cancer ranks the third among malignant tumors worldwide.Gloeostereum incarnatum(GI),a fungus with homology of medicine and food,has multiple pharmacological activities.It was investigated in this study that the anti-colon cancer effect of polysaccharides purifi ed from GI(GIPS)in ApcMinC/Gpt mice(an in situ colon cancer mouse model).Eight-week administration with GIPS at doses of 30 or 90 mg/kg strongly inhibited tumor growth including the reduction on numbers and the suppression of the size without infl uencing the animals’body weight and organ functions.According to the proteomics performing by antibody array,among 308 detected cytokines,GIPS significantly regulated 89 of them.Compared with vehicle-treated mice,GIPS effectively reduced the levels of interleukin(IL)-1β,IL-4,IL-6,IL-17,IL-22,tumor necrosis factor(TNF)-α,matrix metalloproteinase(MMP)-2,and enhanced the levels of IL-15 and IL-18 in serum and/or colon tissues,which suggested its anti-inflammation of GIPS.GIPS suppressed nuclear aggregation of β-catenin,affected the expression of WNT1 and related proteins,thereby regulated the activation of the Wnt signaling.Altogether,GIPS can inhibit the growth of colon cancer,at least partially,via inhibiting the Wnt/β-catenin signaling pathway.展开更多
文摘目的观察石斛水溶性多糖(solub le polysaccharides fromDendrobium candidum,SPD)对在体肿瘤和离体培养肿瘤细胞的抑制作用,探讨其抑制作用的可能机制。方法观察SPD对小鼠接种肉瘤(S180)瘤体生长,离体培养人肝肿瘤细胞(SMMC27721)生长的抑制作用;分析小鼠外周血中超氧物歧化酶活性(superoxide d ismutase,SOD)和膜脂过氧化产物丙二醛(m alond ialdehyde,MDA)含量,小鼠胸腺指数和脾脏指数的变化;核染色(Hoechst染色)法观察SPD对人神经母瘤细胞(SH-SY5Y)细胞凋亡的影响。结果SPD处理能够有效抑制小鼠肉瘤(S180)瘤体生长和离体肝肿瘤细胞生长(P<0.05);有效提高荷瘤小鼠胸腺指数和脾脏指数,提高SOD活性和降低MDA含量(P<0.05);促进神经母瘤细胞凋亡。结论SPD具有显著的抗肿瘤效应,其抑瘤机制可能与SPD促进免疫器官功能和提高抗氧化能力有关。
文摘The present study characterised the types and amounts of non-starch polysaccharides(NSP)remaining undigested along the gastrointestinal tract(GIT)of broiler chickens offered a typical wheat-or maizebased diet.One-day old Cobb 500 mixed-sex chicks were assigned to 24 pens,with 10 birds/pen and 12 pens/treatment.Birds were offered the experimental diets in 3 phases(starter,day 0 to 10;grower,day 11 to 24 and finisher,day 25 to 35).Excreta and digesta samples from the crop,gizzard,duodenum,jejunum,ileum and caeca were collected at 12 and 35 days of age,and analysed for the NSP flow.The wheat-based diet contained higher levels of soluble NSP than the maize-based diet,whereas insoluble NSP levels were similar between the 2 diets.Detailed analysis of NSP constituents revealed that arabinoxylans were the primary NSP in the wheat-based diet,mostly in insoluble form.Pectins were the predominant NSP in the maize-based diet,followed by arabinoxylans.Overall,birds offered the wheatbased diet presented higher levels of soluble NSP remaining in all gut sections compared to birds offered the maize-based diet,at both 12 and 35 days of age(P<0.050).Accumulation of insoluble NSP in the gizzard was noted in birds fed both diets,but was more pronounced in birds offered the maize-based diet compared to the wheat-based diet,at both 12 and 35 days of age(P<0.001).The present study highlights marked differences in the amounts and types of NSP delivered to the different gut sections when feeding wheat-compared to maize-based diets,particularly in the gizzard and the lower GIT of birds.
基金supported by China Agriculture Research System(Grant No.CARS-21)National Natural Science Foundation of China(Grant No.31372089)。
文摘Dendrobium officinale has high medicinal value but grows slowly in natural environment due to its special CAM photosynthetic pathway.In this study,D.officinale were grown aeroponically with light/dark cycles of 12 h/12 h,4 h/4 h,and 2 h/2 h for 150 d.The photosynthetic electron transfer characteristics,photosynthetic CO_(2) fixation pathways,and accumulations of biomass and soluble polysaccharides in D.officinale leaves were studied.The results showed that the photosynthetic apparatus states of D.officinale in aeroponic cultivation under short light/dark cycles of 4 h/4 h and 2 h/2 h were better than that under 12 h/12 h.The dark net CO_(2) exchange percentages of D.officinale were negative in short light/dark cycles of 4 h/4 h and 2 h/2 h,and the daily net CO_(2) exchange amount and dry/fresh weight increases were doubled compared with those in 12 h/12 h light/dark cycle.High soluble polysaccharides content and the soluble polysaccharides yield of D.officinale were obtained in the shorter light/dark cycle of 2 h/2 h.Therefore,the photosynthetic pathway of D.officinale could be switched from CAM to C3 by short light/dark cycles of 4 h/4 h and 2 h/2 h,and its higher biomass accumulation and soluble polysaccharides yield could be obtained by the shorter light/dark cycle of 2 h/2 h in aeroponic cultivation.
文摘目的优选菟丝子多糖提取的最佳工艺并探讨菟丝子多糖在应激状态下对小鼠脑组织脂质过氧化物反应的影响。方法采用正交实验设计方法优选最佳工艺;比色法测定小鼠脑组织超氧化物歧化酶(SOD)和丙二醛(MDA)的含量。结果影响菟丝子多糖提取率的主要因素为提取温度与料液比;不同剂量的菟丝子多糖均能降低MDA的含量(P<0.05),并能增加SOD的活性(P<0.05)。结论菟丝子多糖最佳提取工艺为超声提取,料液比为1∶15,提取温度为80℃,提取时间为60 m in;菟丝子多糖具有抗脂质过氧化保护脑组织的作用。
基金This work was supported by the Ministry of Science and Technology of the People’s Republic of China(Grant No.2018YFE0107800)the“13th Five-year”Science and Technology Projects from Education Department in Jilin Province of P.R.China(Grant No.JJKH20190108KJ)Industrial Technology Research and Development Projects from Development and Reform Commission of Jilin Province(Grant No.2019C050-8).
文摘The incidence rate of colon cancer ranks the third among malignant tumors worldwide.Gloeostereum incarnatum(GI),a fungus with homology of medicine and food,has multiple pharmacological activities.It was investigated in this study that the anti-colon cancer effect of polysaccharides purifi ed from GI(GIPS)in ApcMinC/Gpt mice(an in situ colon cancer mouse model).Eight-week administration with GIPS at doses of 30 or 90 mg/kg strongly inhibited tumor growth including the reduction on numbers and the suppression of the size without infl uencing the animals’body weight and organ functions.According to the proteomics performing by antibody array,among 308 detected cytokines,GIPS significantly regulated 89 of them.Compared with vehicle-treated mice,GIPS effectively reduced the levels of interleukin(IL)-1β,IL-4,IL-6,IL-17,IL-22,tumor necrosis factor(TNF)-α,matrix metalloproteinase(MMP)-2,and enhanced the levels of IL-15 and IL-18 in serum and/or colon tissues,which suggested its anti-inflammation of GIPS.GIPS suppressed nuclear aggregation of β-catenin,affected the expression of WNT1 and related proteins,thereby regulated the activation of the Wnt signaling.Altogether,GIPS can inhibit the growth of colon cancer,at least partially,via inhibiting the Wnt/β-catenin signaling pathway.
