Sodium humate(HA-Na) has been topically used as a wound healing and anti-inflammatory agent in folk medicine. In the present study, HA-Na was investigated for cutaneous wound healing in Sprague–Dawley rats. HA-Na sol...Sodium humate(HA-Na) has been topically used as a wound healing and anti-inflammatory agent in folk medicine. In the present study, HA-Na was investigated for cutaneous wound healing in Sprague–Dawley rats. HA-Na solution(1.0%, w/v) was topically administered to rats undergoing excision wound models. Healing was assessed with a recombinant bovine basic fibroblast growth factor for external use as positive control. Wound healing rates were calculated on Day 3, 6, 9, 14 and 21 after injury, and tissues were also harvested after the same intervals for histological analysis. In addition, tissue hydroxyproline levels were measured. Furthermore, m RNA levels and protein expressions of transforming growth factor-β1, 2, 3(TGF-β1, 2, 3) were determined by RT-PCR and western blot. Protein expression levels of Smad-2,-3,-4 and-7 were also detected by western blot. Our study demonstrates that HA-Na has the capacity to promote wound healing in rats via accelerated wound contraction and increased hydroxyproline content. More importantly, these wound healing effects of HA-Na might be mediated through the TGF-β/Smad signaling pathway. HA-Na may be an effective agent for enhanced wound healing.展开更多
为探讨腐植酸钠对生长猪免疫水平的影响,选择24头健康体重在(26.18±1.32)kg的生长猪,随机分成2组,每组12头,一组设为试验组,另一组设为对照组。连续灌喂10 m l/kg的腐植酸钠(即腐植酸钠总含量100 mg/kg)10 d后,测定T淋巴细胞Ea玫...为探讨腐植酸钠对生长猪免疫水平的影响,选择24头健康体重在(26.18±1.32)kg的生长猪,随机分成2组,每组12头,一组设为试验组,另一组设为对照组。连续灌喂10 m l/kg的腐植酸钠(即腐植酸钠总含量100 mg/kg)10 d后,测定T淋巴细胞Ea玫瑰花环形成率和B淋巴细胞EAC玫瑰花环形成率。结果表明:灌喂腐植酸钠的健康生长猪的Ea玫瑰花环形成率明显高于对照组,差异显著(P<0.05);灌喂腐植酸钠的健康生长猪的EAC玫瑰花环形成率明显高于对照组生长猪,差异极显著(P<0.01)。表明:灌喂腐植酸钠生长猪机体的免疫水平明显提高。展开更多
基金financially supported by Innovation Plan Project of Science and Technology of Shaanxi Province (2013KTCQ03-13)
文摘Sodium humate(HA-Na) has been topically used as a wound healing and anti-inflammatory agent in folk medicine. In the present study, HA-Na was investigated for cutaneous wound healing in Sprague–Dawley rats. HA-Na solution(1.0%, w/v) was topically administered to rats undergoing excision wound models. Healing was assessed with a recombinant bovine basic fibroblast growth factor for external use as positive control. Wound healing rates were calculated on Day 3, 6, 9, 14 and 21 after injury, and tissues were also harvested after the same intervals for histological analysis. In addition, tissue hydroxyproline levels were measured. Furthermore, m RNA levels and protein expressions of transforming growth factor-β1, 2, 3(TGF-β1, 2, 3) were determined by RT-PCR and western blot. Protein expression levels of Smad-2,-3,-4 and-7 were also detected by western blot. Our study demonstrates that HA-Na has the capacity to promote wound healing in rats via accelerated wound contraction and increased hydroxyproline content. More importantly, these wound healing effects of HA-Na might be mediated through the TGF-β/Smad signaling pathway. HA-Na may be an effective agent for enhanced wound healing.
文摘为探讨腐植酸钠对生长猪免疫水平的影响,选择24头健康体重在(26.18±1.32)kg的生长猪,随机分成2组,每组12头,一组设为试验组,另一组设为对照组。连续灌喂10 m l/kg的腐植酸钠(即腐植酸钠总含量100 mg/kg)10 d后,测定T淋巴细胞Ea玫瑰花环形成率和B淋巴细胞EAC玫瑰花环形成率。结果表明:灌喂腐植酸钠的健康生长猪的Ea玫瑰花环形成率明显高于对照组,差异显著(P<0.05);灌喂腐植酸钠的健康生长猪的EAC玫瑰花环形成率明显高于对照组生长猪,差异极显著(P<0.01)。表明:灌喂腐植酸钠生长猪机体的免疫水平明显提高。
