Objective: To study the rules in the renaturation of recombinant antibody. Methods: Anti-TNF-a single domain (Sd) and single chain fragment variety(SCFv) antibodies were separated and purified under four conditions, i...Objective: To study the rules in the renaturation of recombinant antibody. Methods: Anti-TNF-a single domain (Sd) and single chain fragment variety(SCFv) antibodies were separated and purified under four conditions, including cosolvent redoxing, surface active solvent inducting, denaturant solvent inducting and the antigen of rhu TNFL-a inducting. Results: The dissolubility of renaturation products were between 6% - 11 %. Conclusion: These several conditions were good enough to the 2 antibody proteins and the best in them is the combination of the antigen inducting with affinity chromatography.展开更多
基金Supported by National Natural Science Foundation of China, No. 39670695
文摘Objective: To study the rules in the renaturation of recombinant antibody. Methods: Anti-TNF-a single domain (Sd) and single chain fragment variety(SCFv) antibodies were separated and purified under four conditions, including cosolvent redoxing, surface active solvent inducting, denaturant solvent inducting and the antigen of rhu TNFL-a inducting. Results: The dissolubility of renaturation products were between 6% - 11 %. Conclusion: These several conditions were good enough to the 2 antibody proteins and the best in them is the combination of the antigen inducting with affinity chromatography.
