Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ultra-sensitivity.Here,we demonstrate that the electrochemical confined space could efficiently convert s...Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ultra-sensitivity.Here,we demonstrate that the electrochemical confined space could efficiently convert single molecule characteristics into measurable electrochemical signatures with high temporal resolution.The human telomere repeat sequence T8 was used as a probe to determine the electrochemical confined effect in a nanopore.Our results show that the nanopore with comparable confined space of the telomere repeat sequence exhibits the most distinguishable single-molecule signals which suggest the folded conformation of T8.This method will greatly extend the lifetime of a metastable conformation for a single biomolecule by strong analyte-nanopore interactions,which brings the new insight into the understanding of the biomolecule's function at single-molecule level.展开更多
原子力显微镜(Atomic force microscopy,AFM)及荧光显微镜(Fluorescence microscopy,FM)是目前活细胞单分子分析检测中最常用的两种工具。结合两种显微镜的优势,发展高时空分辨、多功能的AFM-FM联用技术成为近年该领域的研究热点。本文...原子力显微镜(Atomic force microscopy,AFM)及荧光显微镜(Fluorescence microscopy,FM)是目前活细胞单分子分析检测中最常用的两种工具。结合两种显微镜的优势,发展高时空分辨、多功能的AFM-FM联用技术成为近年该领域的研究热点。本文简述了AFM单分子力谱和FM单分子荧光成像的原理,总结了AFM-FM联用系统在仪器研制方面的发展概况,并结合本课题组在应用AFM-FM联用技术研究细胞膜上配受体相互作用等方面的工作,介绍了其在活细胞单分子检测中的应用进展。展开更多
基金supported by the National Natural Science Foundation of China(21421004,21505043,21327807)the Fundamental Research Funds for the Central Universities(222201718001,222201717003,222201714012)
文摘Electrochemical analysis of single molecules is a method with the strong ability of the enhanced efficiency and ultra-sensitivity.Here,we demonstrate that the electrochemical confined space could efficiently convert single molecule characteristics into measurable electrochemical signatures with high temporal resolution.The human telomere repeat sequence T8 was used as a probe to determine the electrochemical confined effect in a nanopore.Our results show that the nanopore with comparable confined space of the telomere repeat sequence exhibits the most distinguishable single-molecule signals which suggest the folded conformation of T8.This method will greatly extend the lifetime of a metastable conformation for a single biomolecule by strong analyte-nanopore interactions,which brings the new insight into the understanding of the biomolecule's function at single-molecule level.
文摘原子力显微镜(Atomic force microscopy,AFM)及荧光显微镜(Fluorescence microscopy,FM)是目前活细胞单分子分析检测中最常用的两种工具。结合两种显微镜的优势,发展高时空分辨、多功能的AFM-FM联用技术成为近年该领域的研究热点。本文简述了AFM单分子力谱和FM单分子荧光成像的原理,总结了AFM-FM联用系统在仪器研制方面的发展概况,并结合本课题组在应用AFM-FM联用技术研究细胞膜上配受体相互作用等方面的工作,介绍了其在活细胞单分子检测中的应用进展。
