To search for new bioactive materials which can be used as the substitute of bone repairing and drug carriers, Sema3A-Ioaded chitosan microspheres （SLCM） and silk fibroin （SF） were mixed with calcium sulfate ceme...To search for new bioactive materials which can be used as the substitute of bone repairing and drug carriers, Sema3A-Ioaded chitosan microspheres （SLCM） and silk fibroin （SF） were mixed with calcium sulfate cement （CSC）. SEM, particle size analysis and swelling rate determination were performed to study properties of the microspheres. The drug loading, encapsulation efficiency and drug release rate were determined by ELISA. Microspheres with different SLCM weight contents （0.5%, 1% and 5%） were prepared to determine which one has the strongest mechanical properties and the appropriate setting time. It was revealed that CSC/SF/0.5SLCM has satisfactory mechanical properties, and its in vitro biocompatibility was assessed by MTS. Chitosan microspheres （5-18 μm） were globular, the surface was smooth, and the swelling rate is （77.02 ± 5.57）%. With this formula, the setting time was increased with the addition of SLCM in CSCISF, and the cumulative drug release rate is 44.62% in 28 d. XRD results demonstrate that the main component is calcium sulfate. Also it was found that CSCiSFI 0.5SLCM supports the growth of MC3T3 cells. Thus the preparation of CSCISFIO.5SLCM was reliable, and the products had good structures, physical properties and biocompatibility, appearing to be a promising bone substitute material.展开更多
Diabetic foot ulcers(DFU),which may lead to lower extremity amputation,is one of the severe and chronic complications of diabetic mellitus.This study aims to develop,and use dressings based on Silk fibroin(SF)as the s...Diabetic foot ulcers(DFU),which may lead to lower extremity amputation,is one of the severe and chronic complications of diabetic mellitus.This study aims to develop,and use dressings based on Silk fibroin(SF)as the scaffold material,gelatin microspheres(GMs)as the carrier for the neurotensin(NT),a neuropeptide that acts as an inflammatory modulator in wound healing and NT as accelerate wound healing drug to treat DFU.We evaluated the wound healing processes and neo-tissue formation in rat diabetic model by macroscopic observation,histological observation(H&E staining and Masson's trichrome staining)and immunofluorescence analysis at 3,7,14,21 and 28 post-operation days.Our results show that the NT/GMs/SF group performance the best not only in macroscopic healing and less scars in 28 post-operation days,but also in fibroblast accumulation in tissue granulation,collagen expression and deposition at the wound site.From release profiles,we can know the GMs are a good carrier for control release drugs.The SEM results shows that the NT/GMs/SF dressings have an average pore size are 40–80μm and a porosity of∼85%,this pore size is suit for wound healing regeneration.These results suggest that the NT/GMs/SF dressings may work as an effective support for control release NT to promote DFU wound healing.展开更多
Silk fibroin （SF） is a kind of natural polymers with a great potential in biomedical application. Due to its good biocompatibility, biodegradability and minimal inflammatory reaction, SF is an excellent candidate fo...Silk fibroin （SF） is a kind of natural polymers with a great potential in biomedical application. Due to its good biocompatibility, biodegradability and minimal inflammatory reaction, SF is an excellent candidate for generating tissue engineering scaffolds. Electrospinning is a simple and effective method to fabricate nanofibers, which has several amazing characteristics such as very large surface area to volume ratio, flexibility in surface functionalities, and superior mechanical performance. The electro- spun nanofibers from SF and its blends have been used for varied tissue engineering. This paper will give a brief review about the structure, properties and applications of SF and blend nanofibers via electrospinning.展开更多
In order to investigate the in vitro biocompatibility of a novel polyurethane （PU） membrane modified by incorporation of superfine silk-fibroin powder （SFP）, which was prepared for small-diameter vascular grafts, ...In order to investigate the in vitro biocompatibility of a novel polyurethane （PU） membrane modified by incorporation of superfine silk-fibroin powder （SFP）, which was prepared for small-diameter vascular grafts, with the cultivation of human umbilical vein endothelial cells （HUVECs）, PU and SFP were mixed with the ratios of 9：1, 7：3, 5：5, 3：7 （PU：SFP） to make four composite materials. Unmodified PU and polytetrafluoroethylene （PTFE） were added as control groups. CCK-8 assay was used to evaluate the cytotoxicity of these biomaterials. Data were processed using SPSS, and P〈 0.05 was considered to be statistically significant. Adherence and spreading of HUVECs on the surface of specimens was observed using direct contact cultivation. The toxicity ratings of the novel composites were grade 0-1, which is in the acceptable range. In all the experimental groups except control, SFP/PU with ratio of 1：9 had the least cytotoxicity property, and more content of SFP in the composite showed no improvement of the biocompatibility. HUVECs strongly attached to and grew on the surface of the biomaterials, and proliferated rapidly. The proliferation ability increased with increased proportion of SFP; however the cell quantity on the surface of the materials decreased when the proportion of SFP was equal to or larger than that of PU in the composite. It is concluded that this novel material has excellent cellular affinity with no cytotoxicity to HUVECs. Adding SFP gives PU better biocompatibility, while further research on optimum blend ratios is still needed.展开更多
In this study, the outstanding biocompatibility of silk fibroin （SF） and the highly efficient anti-bacterial effect of nano silver （NS） were utilized to prepare SF/NS composite film with anti- bacterial property. ...In this study, the outstanding biocompatibility of silk fibroin （SF） and the highly efficient anti-bacterial effect of nano silver （NS） were utilized to prepare SF/NS composite film with anti- bacterial property. The structure and property of the film were characterized. The results showed that the structure of SF in the film was mainly silk I. SF in the film was almost insoluble in water. The tensile strength of film with NS was significantly lower than that of films without NS. When the addition of NS was within the range of 0%-0.6%, the elongation at break had no significant difference. The antibacterial rate of the film on staphylococcus aurens and escherichia coil increased with the amount of NS. The minimum amount of NS in the fdm was O. 1% and the maximum amount was 0.5%.展开更多
从废弃蚕茧及缫丝下脚料中提取纯净丝素蛋白,采用盐溶酶解法制备纳米级丝素蛋白（SFP）,按照不同配比与聚乙烯醇（PVA）采用水相混合后制备出嵌入纳米级丝素蛋白颗粒的聚乙烯醇（SFP in PVA）膜,对膜进行表征并测定其促细胞生长性能。使...从废弃蚕茧及缫丝下脚料中提取纯净丝素蛋白,采用盐溶酶解法制备纳米级丝素蛋白（SFP）,按照不同配比与聚乙烯醇（PVA）采用水相混合后制备出嵌入纳米级丝素蛋白颗粒的聚乙烯醇（SFP in PVA）膜,对膜进行表征并测定其促细胞生长性能。使用60%NaSCN溶解丝素蛋白后,采用α-糜蛋白酶、胰蛋白酶、中性蛋白酶进行酶解筛选试验,通过场发射扫描电镜观察,发现中性蛋白酶酶解得到的SFP粉末尺寸约为80~150 nm,颗粒均匀。红外光谱表征确认了SFP/PVA膜的基团间结合;扫描电镜显示SFP以微细线段状镶嵌在PVA膜中。对镶嵌膜的SFP/PVA进行了10/90、20/80、30/70、40/60四种配比的筛选试验,通过膜的表面观察、水中形态稳定性观测和促CHO细胞生长性能测试（MTT法）,结果显示,SFP/PVA配比为30/70的镶嵌膜表面光滑、水中形态稳定,促进CHO细胞快速黏附和生长性能最优。展开更多
Ethosomes are permeation-enhancing carriers, which significantly promote drug delivery into skin. Thus,the introduction of ethosomes into electrospun nanofibers may result in good effect on transdermal drug delivery. ...Ethosomes are permeation-enhancing carriers, which significantly promote drug delivery into skin. Thus,the introduction of ethosomes into electrospun nanofibers may result in good effect on transdermal drug delivery. In this work,a novel ethosome-loaded silk fibroin( SF) /polyethylene oxide( PEO) composite nanofiber was fabricated based on a green electrospinning process. The ethosome-loaded SF /PEO nanofiber was round and smooth as shown by scanning electron microscopy( SEM),and the incorporation of ethosome didn't significantly affect the morphology of the electrospun SF /PEO nanofiber. The data of Fourier transform infrares(FTIR)spectra suggested the existence of ethosome on the SF /PEO nanofiber and the transmission electron microscopy(TEM) clearly showed the distribution of ethosome on the ethosome-loaded SF nanofibers. This ethosome-loaded SF nanofibrous mat may have a promising application in transdermal drug delivery systems.展开更多
Silk fibroin （SF） from the Bombyx mori silkworm exhibits attractive potential applications as biomechanical materials, due to its unique mechanical and biological properties. This review outlines the structure and p...Silk fibroin （SF） from the Bombyx mori silkworm exhibits attractive potential applications as biomechanical materials, due to its unique mechanical and biological properties. This review outlines the structure and properties of SF, including of its biocompatibility and biodegradability. It highlights recent researches on the fabrication of various SF-based composites scaffolds that are promising for tissue engineering applications, and discusses synthetic methods of various SF-based composites scaffolds and valuable approaches for controlling cell behaviors to promote the tissue repair. The function of extracellular matrices and their interaction with cells are also reviewed here.展开更多
After removal of the caries or diseased teeth,the alveolar ridge will undergo absorption and atrophy.When the amount of alveolar bone is insufficient,it will cause an inability to perform effective dental implant rest...After removal of the caries or diseased teeth,the alveolar ridge will undergo absorption and atrophy.When the amount of alveolar bone is insufficient,it will cause an inability to perform effective dental implant restoration.In order to control the absorption and promote the repair and regeneration of alveolar ridge,a method of implanting guided bone regeneration(GBR)membranes at the extraction site is often used.In this study,silk fibroin(SF)and poly-L-lactide lactone(PLCL)were used to prepare bilayered guided bone regeneration membranes,and its morphology,hydrophilicity,surface roughness and mechanical properties were studied.At the same time,the drug release behaviors and cell compatibility of the bilayered membranes were studied.The results showed that SF/PLCL bi-layered membranes had good mechanical properties and surface hydrophilicity,and the drug-loaded bi-layered membranes had good cell compatibility.The bilayered membranes fabricated in this study are of potential for applying in the oral health field to promote bone regeneration.展开更多
The reinforcement of calcium phosphate materials with silk fibroin （SF） has been one of the strategies to overcome the brittleness. However, the lack of osteoinductivity may still restrict their further use. This st...The reinforcement of calcium phosphate materials with silk fibroin （SF） has been one of the strategies to overcome the brittleness. However, the lack of osteoinductivity may still restrict their further use. This study aimed to investigate the biocompatibility and osteogenesis capacity of a novel Semaphorin 3A-loaded chitosan microspheres/SF/a-tricalcium phosphate composite （Sema3A CMs/SF/a-TCP） in vitro. Sema3A was first incorporated into CMs, and the Sema3A CMs/SF/a-TCP composite was then prepared. The morphology of the CMs was observed using SEM. The in vitro release kinetics, cytotoxicity, and cell compatibility were evaluated, and the real-time quantitative polymerase chain reaction （RT-qPCR） and activity of alkaline phosphatase （ALP） were used to evaluate the osteogenesis capacity of the composite. The in vitro release of Sema3A from the Sema3A CMs/SF/a-TCP composite showed a temporally controlled manner. The extract of the Sema3A CMs/SF/a-TCP composite presented no obvious side effect on the MC3T3-E1 cell proliferation, nor promote cell proliferation. The MC3T3-E1 cells were well-spread and presented an elongated shape on the Sema3A CMs/SF/a-TCP composite surface; the ALP activity and the osteogenic-related gene expression were higher than those seeded on the surface of the CMs/SF/a-TCP and SF/a-TCP composites. In conclusion, Sema3A CMslSF/a-TCP has excellent biocompatibility and contributes to the osteoblastic differentiation of MC3T3-E1 cells.展开更多
The electrospun nanofibrous scaffolds made of proteins and polysaccharides were thought to be able to simulate the structure of natural extracellular matrix well.Silk fibroin(SF)and chitosan(CS)are probably the most w...The electrospun nanofibrous scaffolds made of proteins and polysaccharides were thought to be able to simulate the structure of natural extracellular matrix well.Silk fibroin(SF)and chitosan(CS)are probably the most widely used natural materials in biomedical fields including liver tissue engineering for their good properties and wide variety of sources.The asialoglycoprotein receptors of hepatocyte were reported to specifically recognize and interact with galactose.In this work,a green electrospun SF/galactosylated chitosan(GC)composite nanofibrous scaffold was fabricated and characterized.The data indicated that the addition of GC greatly influenced the spinning effect of SF aqueous solution,and the average diameter of the composite nanofibers was about 520nm.Moreover,the green electrospun SF/GC nanofibrous scaffolds were demonstrated significantly enhancing the adhesion and proliferation of hepatocyte(RH35)according to our data.The present study did a useful exploration on constructing scaffolds for liver regeneration by green electrospinning,and also laid a good foundation for the further applicative research of this green electrospun scaffolds in liver tissue engineering.展开更多
This paper was to develop a weft-knitted stent coated by a drng-loaded electro-spun fibrous membrane and then investigate its morphology, mechan/cal properties and in vitro drug release property. This work was started...This paper was to develop a weft-knitted stent coated by a drng-loaded electro-spun fibrous membrane and then investigate its morphology, mechan/cal properties and in vitro drug release property. This work was started by weft-knitting of an inner layer of such stent using polydioxanone （PDO） and silkf＇dment. Subsequently, 5-fluorouracil （5-FU） and curcnmin（CUR） loaded silk fibroin （SF） membranes were coated on the surface of the weft- knitted stent using electro-spinning technique to endow the drug delivery funct/on of the stent. The results show that the radial compression strength and c/renmferentlal expanding strength can reach above （9.1±0.4） cN/cm2 and （205.0± 0.2） cN/mm, respectively. The drug releasing behaviors can be sustained for 400 h. It is concluded that the stents have potential application as anintestinal stent in the future.展开更多
基金Science&Technology Commission of Shanghai Municipality (No. 20DZ2254900)Municipal Public Welfare Research Project from Jiaxing,Zhejiang Province (No. 2022AY10001)Open Project Program of Jiaxing Key Laboratory of Virus-Related Infectious Diseases.
基金Acknowledgements This work was generously supported by the National Natural Science Foundation of China （Grant Nos. 81071454, 81171689 and 81301559）, Department of Orthopedics Clinical Medical Centers in Jiangsu Province （BL2012004）, the Natural Science Foundation of Jiangsu Province （BK20130275）, and the Key Laboratory of Orthopedic Institute of Sooehow University.
文摘To search for new bioactive materials which can be used as the substitute of bone repairing and drug carriers, Sema3A-Ioaded chitosan microspheres （SLCM） and silk fibroin （SF） were mixed with calcium sulfate cement （CSC）. SEM, particle size analysis and swelling rate determination were performed to study properties of the microspheres. The drug loading, encapsulation efficiency and drug release rate were determined by ELISA. Microspheres with different SLCM weight contents （0.5%, 1% and 5%） were prepared to determine which one has the strongest mechanical properties and the appropriate setting time. It was revealed that CSC/SF/0.5SLCM has satisfactory mechanical properties, and its in vitro biocompatibility was assessed by MTS. Chitosan microspheres （5-18 μm） were globular, the surface was smooth, and the swelling rate is （77.02 ± 5.57）%. With this formula, the setting time was increased with the addition of SLCM in CSCISF, and the cumulative drug release rate is 44.62% in 28 d. XRD results demonstrate that the main component is calcium sulfate. Also it was found that CSCiSFI 0.5SLCM supports the growth of MC3T3 cells. Thus the preparation of CSCISFIO.5SLCM was reliable, and the products had good structures, physical properties and biocompatibility, appearing to be a promising bone substitute material.
基金supported financially by the Natural Science Foundation of China(51303064 and 31271019)the Science and Technology Program of Guangzhou(201601010270,2017010160489,201704030083)+1 种基金the Pearl River S&T Nova Program of Guangzhou(201710010155,201806010072)the Science and Technology Project of Guangdong province(2015A010101313,2017A050506011,2017B090911012,2018A050506040,2018A050506019,2018A050506021).
文摘Diabetic foot ulcers(DFU),which may lead to lower extremity amputation,is one of the severe and chronic complications of diabetic mellitus.This study aims to develop,and use dressings based on Silk fibroin(SF)as the scaffold material,gelatin microspheres(GMs)as the carrier for the neurotensin(NT),a neuropeptide that acts as an inflammatory modulator in wound healing and NT as accelerate wound healing drug to treat DFU.We evaluated the wound healing processes and neo-tissue formation in rat diabetic model by macroscopic observation,histological observation(H&E staining and Masson's trichrome staining)and immunofluorescence analysis at 3,7,14,21 and 28 post-operation days.Our results show that the NT/GMs/SF group performance the best not only in macroscopic healing and less scars in 28 post-operation days,but also in fibroblast accumulation in tissue granulation,collagen expression and deposition at the wound site.From release profiles,we can know the GMs are a good carrier for control release drugs.The SEM results shows that the NT/GMs/SF dressings have an average pore size are 40–80μm and a porosity of∼85%,this pore size is suit for wound healing regeneration.These results suggest that the NT/GMs/SF dressings may work as an effective support for control release NT to promote DFU wound healing.
文摘Silk fibroin （SF） is a kind of natural polymers with a great potential in biomedical application. Due to its good biocompatibility, biodegradability and minimal inflammatory reaction, SF is an excellent candidate for generating tissue engineering scaffolds. Electrospinning is a simple and effective method to fabricate nanofibers, which has several amazing characteristics such as very large surface area to volume ratio, flexibility in surface functionalities, and superior mechanical performance. The electro- spun nanofibers from SF and its blends have been used for varied tissue engineering. This paper will give a brief review about the structure, properties and applications of SF and blend nanofibers via electrospinning.
文摘In order to investigate the in vitro biocompatibility of a novel polyurethane （PU） membrane modified by incorporation of superfine silk-fibroin powder （SFP）, which was prepared for small-diameter vascular grafts, with the cultivation of human umbilical vein endothelial cells （HUVECs）, PU and SFP were mixed with the ratios of 9：1, 7：3, 5：5, 3：7 （PU：SFP） to make four composite materials. Unmodified PU and polytetrafluoroethylene （PTFE） were added as control groups. CCK-8 assay was used to evaluate the cytotoxicity of these biomaterials. Data were processed using SPSS, and P〈 0.05 was considered to be statistically significant. Adherence and spreading of HUVECs on the surface of specimens was observed using direct contact cultivation. The toxicity ratings of the novel composites were grade 0-1, which is in the acceptable range. In all the experimental groups except control, SFP/PU with ratio of 1：9 had the least cytotoxicity property, and more content of SFP in the composite showed no improvement of the biocompatibility. HUVECs strongly attached to and grew on the surface of the biomaterials, and proliferated rapidly. The proliferation ability increased with increased proportion of SFP; however the cell quantity on the surface of the materials decreased when the proportion of SFP was equal to or larger than that of PU in the composite. It is concluded that this novel material has excellent cellular affinity with no cytotoxicity to HUVECs. Adding SFP gives PU better biocompatibility, while further research on optimum blend ratios is still needed.
基金National Natural Science Foundations of China，the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘In this study, the outstanding biocompatibility of silk fibroin （SF） and the highly efficient anti-bacterial effect of nano silver （NS） were utilized to prepare SF/NS composite film with anti- bacterial property. The structure and property of the film were characterized. The results showed that the structure of SF in the film was mainly silk I. SF in the film was almost insoluble in water. The tensile strength of film with NS was significantly lower than that of films without NS. When the addition of NS was within the range of 0%-0.6%, the elongation at break had no significant difference. The antibacterial rate of the film on staphylococcus aurens and escherichia coil increased with the amount of NS. The minimum amount of NS in the fdm was O. 1% and the maximum amount was 0.5%.
文摘从废弃蚕茧及缫丝下脚料中提取纯净丝素蛋白,采用盐溶酶解法制备纳米级丝素蛋白（SFP）,按照不同配比与聚乙烯醇（PVA）采用水相混合后制备出嵌入纳米级丝素蛋白颗粒的聚乙烯醇（SFP in PVA）膜,对膜进行表征并测定其促细胞生长性能。使用60%NaSCN溶解丝素蛋白后,采用α-糜蛋白酶、胰蛋白酶、中性蛋白酶进行酶解筛选试验,通过场发射扫描电镜观察,发现中性蛋白酶酶解得到的SFP粉末尺寸约为80~150 nm,颗粒均匀。红外光谱表征确认了SFP/PVA膜的基团间结合;扫描电镜显示SFP以微细线段状镶嵌在PVA膜中。对镶嵌膜的SFP/PVA进行了10/90、20/80、30/70、40/60四种配比的筛选试验,通过膜的表面观察、水中形态稳定性观测和促CHO细胞生长性能测试（MTT法）,结果显示,SFP/PVA配比为30/70的镶嵌膜表面光滑、水中形态稳定,促进CHO细胞快速黏附和生长性能最优。
基金Natural Science Foundation of Shanghai,China(No.12ZR1400300)Fundamental Research Funds for the Central Universities of China+1 种基金"111 Project"Biomedical Textile Materials Science and Technology of China(No.B07024)Open Foundation of State Key Laboratory for Modification of Chemical Fibers and Polymer Materials(No.LK1111)
文摘Ethosomes are permeation-enhancing carriers, which significantly promote drug delivery into skin. Thus,the introduction of ethosomes into electrospun nanofibers may result in good effect on transdermal drug delivery. In this work,a novel ethosome-loaded silk fibroin( SF) /polyethylene oxide( PEO) composite nanofiber was fabricated based on a green electrospinning process. The ethosome-loaded SF /PEO nanofiber was round and smooth as shown by scanning electron microscopy( SEM),and the incorporation of ethosome didn't significantly affect the morphology of the electrospun SF /PEO nanofiber. The data of Fourier transform infrares(FTIR)spectra suggested the existence of ethosome on the SF /PEO nanofiber and the transmission electron microscopy(TEM) clearly showed the distribution of ethosome on the ethosome-loaded SF nanofibers. This ethosome-loaded SF nanofibrous mat may have a promising application in transdermal drug delivery systems.
文摘Silk fibroin （SF） from the Bombyx mori silkworm exhibits attractive potential applications as biomechanical materials, due to its unique mechanical and biological properties. This review outlines the structure and properties of SF, including of its biocompatibility and biodegradability. It highlights recent researches on the fabrication of various SF-based composites scaffolds that are promising for tissue engineering applications, and discusses synthetic methods of various SF-based composites scaffolds and valuable approaches for controlling cell behaviors to promote the tissue repair. The function of extracellular matrices and their interaction with cells are also reviewed here.
基金Natural Science Foundation for Key Program of the Jiangsu Higher Education Institutions,China(No.19KJA610004)Natural Science Foundation of Jiangsu Province,China(No.BK20161254)+1 种基金China Postdoctoral Science Foundation(No.2019M651947)Mandatory Projects of Nantong Municipal Science and Technology Plan,China(No.JC2018004)
文摘After removal of the caries or diseased teeth,the alveolar ridge will undergo absorption and atrophy.When the amount of alveolar bone is insufficient,it will cause an inability to perform effective dental implant restoration.In order to control the absorption and promote the repair and regeneration of alveolar ridge,a method of implanting guided bone regeneration(GBR)membranes at the extraction site is often used.In this study,silk fibroin(SF)and poly-L-lactide lactone(PLCL)were used to prepare bilayered guided bone regeneration membranes,and its morphology,hydrophilicity,surface roughness and mechanical properties were studied.At the same time,the drug release behaviors and cell compatibility of the bilayered membranes were studied.The results showed that SF/PLCL bi-layered membranes had good mechanical properties and surface hydrophilicity,and the drug-loaded bi-layered membranes had good cell compatibility.The bilayered membranes fabricated in this study are of potential for applying in the oral health field to promote bone regeneration.
文摘The reinforcement of calcium phosphate materials with silk fibroin （SF） has been one of the strategies to overcome the brittleness. However, the lack of osteoinductivity may still restrict their further use. This study aimed to investigate the biocompatibility and osteogenesis capacity of a novel Semaphorin 3A-loaded chitosan microspheres/SF/a-tricalcium phosphate composite （Sema3A CMs/SF/a-TCP） in vitro. Sema3A was first incorporated into CMs, and the Sema3A CMs/SF/a-TCP composite was then prepared. The morphology of the CMs was observed using SEM. The in vitro release kinetics, cytotoxicity, and cell compatibility were evaluated, and the real-time quantitative polymerase chain reaction （RT-qPCR） and activity of alkaline phosphatase （ALP） were used to evaluate the osteogenesis capacity of the composite. The in vitro release of Sema3A from the Sema3A CMs/SF/a-TCP composite showed a temporally controlled manner. The extract of the Sema3A CMs/SF/a-TCP composite presented no obvious side effect on the MC3T3-E1 cell proliferation, nor promote cell proliferation. The MC3T3-E1 cells were well-spread and presented an elongated shape on the Sema3A CMs/SF/a-TCP composite surface; the ALP activity and the osteogenic-related gene expression were higher than those seeded on the surface of the CMs/SF/a-TCP and SF/a-TCP composites. In conclusion, Sema3A CMslSF/a-TCP has excellent biocompatibility and contributes to the osteoblastic differentiation of MC3T3-E1 cells.
基金“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)Natural Science Foundation of Shanghai,China(No.12ZR1400300)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry
文摘The electrospun nanofibrous scaffolds made of proteins and polysaccharides were thought to be able to simulate the structure of natural extracellular matrix well.Silk fibroin(SF)and chitosan(CS)are probably the most widely used natural materials in biomedical fields including liver tissue engineering for their good properties and wide variety of sources.The asialoglycoprotein receptors of hepatocyte were reported to specifically recognize and interact with galactose.In this work,a green electrospun SF/galactosylated chitosan(GC)composite nanofibrous scaffold was fabricated and characterized.The data indicated that the addition of GC greatly influenced the spinning effect of SF aqueous solution,and the average diameter of the composite nanofibers was about 520nm.Moreover,the green electrospun SF/GC nanofibrous scaffolds were demonstrated significantly enhancing the adhesion and proliferation of hepatocyte(RH35)according to our data.The present study did a useful exploration on constructing scaffolds for liver regeneration by green electrospinning,and also laid a good foundation for the further applicative research of this green electrospun scaffolds in liver tissue engineering.
基金National Natural Science Foundation of China(No.51603140)Natural Science Foundation of Jiangsu Province,China(No.BK20150372)+2 种基金University Science Research Project of Jiangsu Province,China(No.16KJB540003)Key Industry Technology Innovation,Science and Technology Project of Suzhou,China(No.SYG201638)Sino-Germany Joint Project,China(No.GZ1094)
文摘This paper was to develop a weft-knitted stent coated by a drng-loaded electro-spun fibrous membrane and then investigate its morphology, mechan/cal properties and in vitro drug release property. This work was started by weft-knitting of an inner layer of such stent using polydioxanone （PDO） and silkf＇dment. Subsequently, 5-fluorouracil （5-FU） and curcnmin（CUR） loaded silk fibroin （SF） membranes were coated on the surface of the weft- knitted stent using electro-spinning technique to endow the drug delivery funct/on of the stent. The results show that the radial compression strength and c/renmferentlal expanding strength can reach above （9.1±0.4） cN/cm2 and （205.0± 0.2） cN/mm, respectively. The drug releasing behaviors can be sustained for 400 h. It is concluded that the stents have potential application as anintestinal stent in the future.
