目的:探讨细菌性阴道病(bacteria l vag inos is,BV)BV蓝快速检测在临床中的应用价值。方法对340例阴道分泌物标本平行进行Am se l法[1](颜色和性状观察、胺试验、pH值和镜检线索细胞)、两种BV蓝快速法检测。以Am se l法合并革兰染色法...目的:探讨细菌性阴道病(bacteria l vag inos is,BV)BV蓝快速检测在临床中的应用价值。方法对340例阴道分泌物标本平行进行Am se l法[1](颜色和性状观察、胺试验、pH值和镜检线索细胞)、两种BV蓝快速法检测。以Am se l法合并革兰染色法为扩大了的金标准,评价BV蓝快速检测法的临床应用价值。结果BV蓝快速检测法A与Am se l法结合涂片革兰染色法在临床细菌性阴道炎检测中无显著差异(A试剂χ2=0.167,P>0.05),BV蓝快速检测法B与Am se l法结合涂片革兰染色法在临床细菌性阴道炎检测中差异有非常显著意义(χ2=14.75,P≤0.01)。结论BV蓝快速检测法是一种新型、简便、快速、经济的方法,可用于临床辅助诊断BV,但是市场上的试剂质量良莠不齐,要认真比对,筛选出好的试剂才能做到真正意义的快速临床辅助诊断,减少漏诊误诊率。展开更多
The use of oseltamivir, widely stockpiled as one of the drugs for use in a possible avian influenza pandemic, has been reported to be associated with neuropsychiatric disorders and severe skin reactions, primarily in ...The use of oseltamivir, widely stockpiled as one of the drugs for use in a possible avian influenza pandemic, has been reported to be associated with neuropsychiatric disorders and severe skin reactions, primarily in Japan. Here we identified a nonsynonymous SNP （single nucleotide polymorphism） in dbSNP database, R41Q, near the enzymatic active site of human cytosolic sialidase, a homologue of virus neuraminidase that is the target of oseltamivir. This SNP occurred in 9.29% of Asian population and none of European and African American population. Our structural analyses and Ki measurements using in vitro sialidase assays indicated that this SNP could increase the unintended binding affinity of human sialidase to oseltamivir carboxylate, the active form of oseltamivir, thus reducing sialidase activity. In addition, this SNP itself results in an enzyme with an intrinsically lower sialidase activity, as shown by its increased Km and decreased Vmax values. Theoretically administration of oseltamivir to people with this SNP might further reduce their sialidase activity. We note the similarity between the reported neuropsychiatric side effects ofoseltamivir and the known symptoms of human sialidase-related disorders. We propose that this Asian-enriched sialidase variation caused by the SNP, likely in homozygous form, may be associated with certain severe adverse reactions to oseltamivir.展开更多
文摘目的:探讨细菌性阴道病(bacteria l vag inos is,BV)BV蓝快速检测在临床中的应用价值。方法对340例阴道分泌物标本平行进行Am se l法[1](颜色和性状观察、胺试验、pH值和镜检线索细胞)、两种BV蓝快速法检测。以Am se l法合并革兰染色法为扩大了的金标准,评价BV蓝快速检测法的临床应用价值。结果BV蓝快速检测法A与Am se l法结合涂片革兰染色法在临床细菌性阴道炎检测中无显著差异(A试剂χ2=0.167,P>0.05),BV蓝快速检测法B与Am se l法结合涂片革兰染色法在临床细菌性阴道炎检测中差异有非常显著意义(χ2=14.75,P≤0.01)。结论BV蓝快速检测法是一种新型、简便、快速、经济的方法,可用于临床辅助诊断BV,但是市场上的试剂质量良莠不齐,要认真比对,筛选出好的试剂才能做到真正意义的快速临床辅助诊断,减少漏诊误诊率。
文摘The use of oseltamivir, widely stockpiled as one of the drugs for use in a possible avian influenza pandemic, has been reported to be associated with neuropsychiatric disorders and severe skin reactions, primarily in Japan. Here we identified a nonsynonymous SNP （single nucleotide polymorphism） in dbSNP database, R41Q, near the enzymatic active site of human cytosolic sialidase, a homologue of virus neuraminidase that is the target of oseltamivir. This SNP occurred in 9.29% of Asian population and none of European and African American population. Our structural analyses and Ki measurements using in vitro sialidase assays indicated that this SNP could increase the unintended binding affinity of human sialidase to oseltamivir carboxylate, the active form of oseltamivir, thus reducing sialidase activity. In addition, this SNP itself results in an enzyme with an intrinsically lower sialidase activity, as shown by its increased Km and decreased Vmax values. Theoretically administration of oseltamivir to people with this SNP might further reduce their sialidase activity. We note the similarity between the reported neuropsychiatric side effects ofoseltamivir and the known symptoms of human sialidase-related disorders. We propose that this Asian-enriched sialidase variation caused by the SNP, likely in homozygous form, may be associated with certain severe adverse reactions to oseltamivir.
