The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present stu...The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present study aimed to evaluate whether early cumulus cell removal could affect in vitro fertilization outcomes. Based on a history of infertility and semen analysis, 61 patients were identified, and the cumulus cells were mechanically removed at either6 h post-insemination (Group A, 387 mature oocytes) or 18 - 20 h post-insemination as traditional in vitro fertilization (Group B, 424 mature oocytes). There were no significant differences in the rates of fertilization, available embryos, high quality embryos, birth, premature birth, pregnancy, or implantation when we compared the two groups. However, Group A exhibited a significantly higher polyspermy rate, and significantly lower rates of blastocyst formation and high-quality blastocyst formation, compared with Group B. Therefore, the results of present study indicate that early cumulus cell removal (at 6 h post-insemination) may negatively affect the rates of polyspermy, blastocyst formation, and high-quality blastocyst formation.展开更多
In conventional in vitro fertilization（IVF）.since sperm metabolites,granular cells and death spermatozoa may consume lots of energy in the culture medium due to longer co-incubation of oocytes and high concentration...In conventional in vitro fertilization（IVF）.since sperm metabolites,granular cells and death spermatozoa may consume lots of energy in the culture medium due to longer co-incubation of oocytes and high concentration spermatozoa.The oocytes are in innutritive environment,which leads to the hardening of oocyte plasma membrane. At the same time,the high levels of estradiol（E2） and progestone（P） produced by granular cells have direct toxic effects to affect embryo cleavage,development and implantation.Therefore,short-term insemination is adopted in more and more reproductive centers. 1.Short-term insemination may increase oocyte-utilization rate,high-quality embryo rate and embryo-utilization rate.2.Retention of cumulus cells may reduce polyspermic fertilization rate.Studies have indicated that the polyspermic fertilization rate is significantly higher in cumulus cell-free group than in cumulus cell group.In short-term insemination,the remaining oocytes should retain cumulus cells to reduce polyspermic fertilization under the circumstance of successful fertilization.3.There is no significant difference in 2PN embryo chromosome abnormality between conventional IVF group and short-term insemination group.4.Short-term insemination may significantly decrease ICSI rate and partial ICSI rate.5.Complete fertilization failure rate significantly decreases in short-term insemination. Short-term insemination reduces unfavourable factors for embryo development,therefore increases high quality embryo rate.If short-term insemination is adopted in IVF,under the circumstance of successful fertilization,the remaining oocytes should retain cumulus cells as much as possible to reduce polyspermic fertilization,improve oocyteutilization rate and optimize IVF outcomes.展开更多
文摘The short-term insemination technique has been widely applied, although it is not clear whether the mechanical desorption of cumulus cells can affect normal fertilization and birth outcomes. Therefore, the present study aimed to evaluate whether early cumulus cell removal could affect in vitro fertilization outcomes. Based on a history of infertility and semen analysis, 61 patients were identified, and the cumulus cells were mechanically removed at either6 h post-insemination (Group A, 387 mature oocytes) or 18 - 20 h post-insemination as traditional in vitro fertilization (Group B, 424 mature oocytes). There were no significant differences in the rates of fertilization, available embryos, high quality embryos, birth, premature birth, pregnancy, or implantation when we compared the two groups. However, Group A exhibited a significantly higher polyspermy rate, and significantly lower rates of blastocyst formation and high-quality blastocyst formation, compared with Group B. Therefore, the results of present study indicate that early cumulus cell removal (at 6 h post-insemination) may negatively affect the rates of polyspermy, blastocyst formation, and high-quality blastocyst formation.
文摘In conventional in vitro fertilization（IVF）.since sperm metabolites,granular cells and death spermatozoa may consume lots of energy in the culture medium due to longer co-incubation of oocytes and high concentration spermatozoa.The oocytes are in innutritive environment,which leads to the hardening of oocyte plasma membrane. At the same time,the high levels of estradiol（E2） and progestone（P） produced by granular cells have direct toxic effects to affect embryo cleavage,development and implantation.Therefore,short-term insemination is adopted in more and more reproductive centers. 1.Short-term insemination may increase oocyte-utilization rate,high-quality embryo rate and embryo-utilization rate.2.Retention of cumulus cells may reduce polyspermic fertilization rate.Studies have indicated that the polyspermic fertilization rate is significantly higher in cumulus cell-free group than in cumulus cell group.In short-term insemination,the remaining oocytes should retain cumulus cells to reduce polyspermic fertilization under the circumstance of successful fertilization.3.There is no significant difference in 2PN embryo chromosome abnormality between conventional IVF group and short-term insemination group.4.Short-term insemination may significantly decrease ICSI rate and partial ICSI rate.5.Complete fertilization failure rate significantly decreases in short-term insemination. Short-term insemination reduces unfavourable factors for embryo development,therefore increases high quality embryo rate.If short-term insemination is adopted in IVF,under the circumstance of successful fertilization,the remaining oocytes should retain cumulus cells as much as possible to reduce polyspermic fertilization,improve oocyteutilization rate and optimize IVF outcomes.
