供体牛超排后用富含X-精子的性控精液人工授精是生产奶牛体内性控胚胎的有效方法。本研究旨在探索冷冻X-精液不同输精方法对蒙贝利亚×荷斯坦F1体内性控胚胎生产的影响。选择10头蒙贝利亚×荷斯坦F1青年母牛作供体,按4 d 8次递...供体牛超排后用富含X-精子的性控精液人工授精是生产奶牛体内性控胚胎的有效方法。本研究旨在探索冷冻X-精液不同输精方法对蒙贝利亚×荷斯坦F1体内性控胚胎生产的影响。选择10头蒙贝利亚×荷斯坦F1青年母牛作供体,按4 d 8次递减方法肌肉注射FSH超排处理,发情后分别在12、24 h和36 h各输精1次,每次输精剂量分别为2剂、2剂和1剂0.25 mL细管冷冻X-精液(实验Ⅰ组),或者分别在发情12 h和24 h各输精1次,相应的输精剂量为2剂0.25 mL细管冷冻X-精液(实验Ⅱ组)。配种后第7天(发情当天为0 d)常规方法采集胚胎。结果表明:实验Ⅰ组和实验Ⅱ组供体牛头均胚胎总数(10.0 vs 11.0)、头均可用胚胎数(6.2 vs 6.4)和头均不可用胚胎数(3.8 vs 4.6)差异不显著(P>0.05);虽然两实验组之间可用胚胎比例(62.0%vs 58.2%)和不可用胚胎比例(38.0%vs 41.8%)差异不显著,但实验Ⅰ组头均不受精卵数极显著低于实验Ⅱ组(P<0.01)。因此,供体母牛超排发情后适当增加人工授精配种次数可减少不受精卵的比例。展开更多
The antioxidant of reduced glutathione（GSH） is the most abundant thiol in cells for the maintenance of the intracellular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubati...The antioxidant of reduced glutathione（GSH） is the most abundant thiol in cells for the maintenance of the intracellular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubation with oocytes on the development potential of embryos obtained by in vitro fertilization（IVF）. Also the mitochondrial membrane potential（ΔΨm）, plasma membrane integrity（viability）, DNA fragmentation, reactive oxygen species（ROS） content, superoxide dismutase（SOD）, catalase（CAT） and glutathione peroxidase（GPx） activities, methane dicarboxylic aldehyde（MDA） level as indices of lipid peroxidation in sex-sorted and unsorted sperm from three bulls were investigated using flow cytometry and enzyme-labeled instrument individually. The results showed that 2 mm ol L^–1 GSH increased significantly the cleavage rate（86.68% vs. 82.78%）, 4- to 8-cell rate（82.30% vs. 73.43%） and blastocyst rate（43.15% vs. 35.24%） of IVF embryos compared with untreated group. Furthermore, addition of GSH increased significantly the ΔΨm and viability, decreased the ratio of DNA fragmentation in sex-sorted or unsorted semen（P〈0.05）, except the sex-sorted semen from bull 019. Similarly, activities of SOD, CAT and GPx were increased significantly. However, the contents of MDA were decreased significantly both in sex-sorted and unsorted semen treated with GSH（P〈0.05）. These results suggest that sperm pretreatment with GSH during IVF can maintain better the viability and fertility of sperm through reducing apoptosis and increasing the antioxidant capacity, which improves the IVF embryos development.展开更多
文摘供体牛超排后用富含X-精子的性控精液人工授精是生产奶牛体内性控胚胎的有效方法。本研究旨在探索冷冻X-精液不同输精方法对蒙贝利亚×荷斯坦F1体内性控胚胎生产的影响。选择10头蒙贝利亚×荷斯坦F1青年母牛作供体,按4 d 8次递减方法肌肉注射FSH超排处理,发情后分别在12、24 h和36 h各输精1次,每次输精剂量分别为2剂、2剂和1剂0.25 mL细管冷冻X-精液(实验Ⅰ组),或者分别在发情12 h和24 h各输精1次,相应的输精剂量为2剂0.25 mL细管冷冻X-精液(实验Ⅱ组)。配种后第7天(发情当天为0 d)常规方法采集胚胎。结果表明:实验Ⅰ组和实验Ⅱ组供体牛头均胚胎总数(10.0 vs 11.0)、头均可用胚胎数(6.2 vs 6.4)和头均不可用胚胎数(3.8 vs 4.6)差异不显著(P>0.05);虽然两实验组之间可用胚胎比例(62.0%vs 58.2%)和不可用胚胎比例(38.0%vs 41.8%)差异不显著,但实验Ⅰ组头均不受精卵数极显著低于实验Ⅱ组(P<0.01)。因此,供体母牛超排发情后适当增加人工授精配种次数可减少不受精卵的比例。
基金supported by the grants from the National Science and Technology Support Program of China (2012BAD12B01)the Basic Research Fund of Institute of Animal Science, Chinese Academy of Agricultural Sciences (2013ywf-zd-2)+1 种基金the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS06)the China Agriculture Research System (CARS-37)
文摘The antioxidant of reduced glutathione（GSH） is the most abundant thiol in cells for the maintenance of the intracellular redox balance. The study aimed to assay the effect of sperm treatment with GSH before incubation with oocytes on the development potential of embryos obtained by in vitro fertilization（IVF）. Also the mitochondrial membrane potential（ΔΨm）, plasma membrane integrity（viability）, DNA fragmentation, reactive oxygen species（ROS） content, superoxide dismutase（SOD）, catalase（CAT） and glutathione peroxidase（GPx） activities, methane dicarboxylic aldehyde（MDA） level as indices of lipid peroxidation in sex-sorted and unsorted sperm from three bulls were investigated using flow cytometry and enzyme-labeled instrument individually. The results showed that 2 mm ol L^–1 GSH increased significantly the cleavage rate（86.68% vs. 82.78%）, 4- to 8-cell rate（82.30% vs. 73.43%） and blastocyst rate（43.15% vs. 35.24%） of IVF embryos compared with untreated group. Furthermore, addition of GSH increased significantly the ΔΨm and viability, decreased the ratio of DNA fragmentation in sex-sorted or unsorted semen（P〈0.05）, except the sex-sorted semen from bull 019. Similarly, activities of SOD, CAT and GPx were increased significantly. However, the contents of MDA were decreased significantly both in sex-sorted and unsorted semen treated with GSH（P〈0.05）. These results suggest that sperm pretreatment with GSH during IVF can maintain better the viability and fertility of sperm through reducing apoptosis and increasing the antioxidant capacity, which improves the IVF embryos development.
