The spermatozoal physiological characteristrics of four species of seawater fish, sea bass (Lateolabrax japonicus), black porgy (Sparus macrocephalus),mudskipper (Boleophthalmus pectirostris) and red sea bream (Pagros...The spermatozoal physiological characteristrics of four species of seawater fish, sea bass (Lateolabrax japonicus), black porgy (Sparus macrocephalus),mudskipper (Boleophthalmus pectirostris) and red sea bream (Pagrosomus major), were investigated. Both the spermatozoa survival rate and swimming time were taken as criterions to evaluate the effects of various salinities and pH on the spermatozoa. The results indicated that different salinities and pH affected chiefly the swimming time of the four species of fish spermatozoa. The most suitable salinities for the mudskipper ranged from 15 to 20 for the sea bass, about 20 for the black porgy, about 25 for the red sea bream, about 30. The most appropriate pH for the sea bass, black porgy and red sea bream was about 8.0, but for the mudskipper was 6.02 8.01. Under the appropriate enviromental conditions, the swimming time of the sea bass, black porgy and red sea bream spermatozoa lasted 20 minutes, while the mudskipper ones lasted from 80 to 90 minutes.展开更多
采用反相高效液相色谱法,以甲醇(49+1)为流动相,对维生素A、D和E混标进行高速紫外扫描,在Nova Pak C18柱上获得分离良好的三维图谱。据此,选择维生素A、D和E各自最大吸收波长325、265和290nm作为检测波长。实验结果表明:无骨海鱼中维生...采用反相高效液相色谱法,以甲醇(49+1)为流动相,对维生素A、D和E混标进行高速紫外扫描,在Nova Pak C18柱上获得分离良好的三维图谱。据此,选择维生素A、D和E各自最大吸收波长325、265和290nm作为检测波长。实验结果表明:无骨海鱼中维生素A、D和E的质量分数分别为0.80、0.17、4.14mg kg,相对标准偏差为3.7%～5.0%。质量浓度0.10～10mg L范围内,维生素A、D、E均呈现良好的线性关系,检出限为0.05～0.62mg L,回收率在90.0%～110%之间。展开更多
文摘The spermatozoal physiological characteristrics of four species of seawater fish, sea bass (Lateolabrax japonicus), black porgy (Sparus macrocephalus),mudskipper (Boleophthalmus pectirostris) and red sea bream (Pagrosomus major), were investigated. Both the spermatozoa survival rate and swimming time were taken as criterions to evaluate the effects of various salinities and pH on the spermatozoa. The results indicated that different salinities and pH affected chiefly the swimming time of the four species of fish spermatozoa. The most suitable salinities for the mudskipper ranged from 15 to 20 for the sea bass, about 20 for the black porgy, about 25 for the red sea bream, about 30. The most appropriate pH for the sea bass, black porgy and red sea bream was about 8.0, but for the mudskipper was 6.02 8.01. Under the appropriate enviromental conditions, the swimming time of the sea bass, black porgy and red sea bream spermatozoa lasted 20 minutes, while the mudskipper ones lasted from 80 to 90 minutes.
