Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ...Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.展开更多
<span style="font-family:Verdana;">This study examined the relationship between tree frog</span> (<i><i><span style="font-family:Verdana;">Hyla</span></i>&...<span style="font-family:Verdana;">This study examined the relationship between tree frog</span> (<i><i><span style="font-family:Verdana;">Hyla</span></i><span> <i><span style="font-family:Verdana;">savignyi</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">)</span><span style="font-family:Verdana;"> coloring and its different seasonal habitats at the southern border of its distribution. The results show that tree frog color is affected by the dominant colors in its habitat, which vary seasonally, especially between winter and summer. Tree frog colors were various shades of green, white, brown, and black. No genetic marker was found to characterize the color. The ability of a small frog to infer its own time with the help of color changes occurring in the habitat on the southern border of its distribution, which are relatively broad, gives this species an advantage.</span>展开更多
基金funded by the National Key Research and Development Program of China(Nos.2019YFE0190900,2018YFD0900705).
文摘Actin is a ubiquitous protein and plays essential roles on cellular structure maintenance and cellular motility in both muscle and non-muscle tissues.Multiple genes encoding muscle actin have been identified from the ascidians,including those expressed in the larval tail muscle,the adult body-wall muscle,and adult heart muscle.In this study,a novel striated non-tail muscle actin gene was identified from the RNA-seq data of Ciona savignyi embryos.Phylogenetic analysis,alignment of the N-terminal amino acid sequences and comparation of diagnostic residues provided evidence that it had high similarity with vertebrate cardiac and skeletal muscle actin.In situ hybridization and promoter-driven GFP reporter assay revealed that it was specifically expressed in the primordia of the oral and atrial siphon.We hereby defined it as siphon-specific muscle actin coding gene(Cs-SMA).A 201 bp(−1350 bp to−1150 bp)sequence containing T-box and Six1/2 binding motif within the upstream region of Cs-SMA confined the expression of GFP in the siphons of electroporated embryos.Six1/2 binding motif was experimentally confirmed to play indispensable role in controlling the siphon-specific expression of Cs-SMA.The tissue-specific expression of Cs-SMA in the siphon primordia indicated its potential crucial roles in Ciona embryogenesis and organogenesis.
文摘<span style="font-family:Verdana;">This study examined the relationship between tree frog</span> (<i><i><span style="font-family:Verdana;">Hyla</span></i><span> <i><span style="font-family:Verdana;">savignyi</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">)</span><span style="font-family:Verdana;"> coloring and its different seasonal habitats at the southern border of its distribution. The results show that tree frog color is affected by the dominant colors in its habitat, which vary seasonally, especially between winter and summer. Tree frog colors were various shades of green, white, brown, and black. No genetic marker was found to characterize the color. The ability of a small frog to infer its own time with the help of color changes occurring in the habitat on the southern border of its distribution, which are relatively broad, gives this species an advantage.</span>
