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Physicochemical Properties and Nutritional Ingredients of Kernel Oil of <i>Carya cathayensis</i>Sarg 被引量:5
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作者 Lisong Hu Menghao Du Jingping Zhang 《American Journal of Plant Sciences》 2018年第12期2494-2503,共10页
Carya cathayensis Sarg is distributed near Tianmu mountain areas in Zhejiang Province and Anhui Province, China. Kernel of Carya cathayensis Sarg, which is well-known for the daintiness and nutritional content, is pop... Carya cathayensis Sarg is distributed near Tianmu mountain areas in Zhejiang Province and Anhui Province, China. Kernel of Carya cathayensis Sarg, which is well-known for the daintiness and nutritional content, is popular and very delicious food in eastern China. In this paper, we investigated the physiochemical properties and nutrient gradients of the kernel oil of Carya cathayensis Sarg. The results showed that the kernel oil of Carya cathayensis Sarg contained 63% of fat, 9.1% of proteins, 3.6% of starch and 4.6% of soluble proteins (based ration of weight). The compositions of the fatty acids were very similar to olive oil, and the total unsaturated fatty acids were 94%. Meanwhile, the kernel oil of Carya cathayensis Sarg also contained some polyphenols such as vanillic acid, ferulic acid, 3-methoxybenzoic acid and quercetin. The content of total polyphenols was 9.7 μg/g. Furthermore, the kernel oil of Carya cathayensis Sarg also contained some trace amount of squalene, β-Sitosterol, Vitamin E. 展开更多
关键词 CARYA cathayensis sarg KERNEL OIL Compositions FATTY Acids
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Effect of <i>Carya cathayensis Sarg</i>Shell Substrate on Yield and Nutrient Amount of <i>Pleurotus geesteranus</i> 被引量:2
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作者 Jinping Zhang Xuebin Li +1 位作者 Yue Ying Xiaohua Yao 《Journal of Geoscience and Environment Protection》 2019年第11期11-23,共13页
Using cottonseed hull as the control substrate, different proportions of the untreated and treated Carya cathayensis Sarg shells were added to cultivation of Pleurotus geesteranus. The mycelial growth rate, yield, nut... Using cottonseed hull as the control substrate, different proportions of the untreated and treated Carya cathayensis Sarg shells were added to cultivation of Pleurotus geesteranus. The mycelial growth rate, yield, nutritional composition and contents of heavy metals of the Pleurotus geesteranus cultivated on these substrates were determined. The results suggest that added to treated Carya cathayensis Sarg shell promoted the mycelial growth and increased the yield, biological efficiency and the contents of crude fiber, ash, amino acids and essential amino acids of Pleurotus geesteranus. In addition, the fat content and soluble sugar contents of Pleurotus geesteranus were decreased with the increase of the Carya cathayensis Sarg shell content in substrate, but their contents of heavy metals including mercury (Hg), arsenic (As), cadmium (Cd) and lead (Pb) were higher than that obtained on control substrate. 展开更多
关键词 CARYA cathayensis sarg SHELL PLEUROTUS geesteranus Mycelial Growth Rate YIELD Nutrients
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Optimization on Ultrasonic Extraction Method of Total Flavonoids for Liriodendron chinense Sarg.×L.tulipifera L. by Response Surface Methodology (RSM) 被引量:1
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作者 Yi JIANG Xuqin REN +2 位作者 Gang LI Guoqing PAN Run ZHUO 《Agricultural Biotechnology》 CAS 2019年第5期132-135,共4页
With the leaves,bark and roots of Liriodendron chinense Sarg.× L.tulipifera L.as experiment materials,the ultrasonic-assisted extraction conditions of total flavonoids were optimized by response surface Box-Behnk... With the leaves,bark and roots of Liriodendron chinense Sarg.× L.tulipifera L.as experiment materials,the ultrasonic-assisted extraction conditions of total flavonoids were optimized by response surface Box-Behnken test design.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from the leaves of L.chinense Sarg.× L.tulipifera L.;ultrasonic temperature and liquid-to-solid ratio had an interaction on the extraction amount of flavonoids;and the optimum ultrasonic time,temperature and liquid-to-solid ratio for the extraction of flavonoids from leaves of L.chinense Sarg.× L.tulipifera L.were 19.82 min,28.60 ℃ and 9.48 ml/g,respectively.Ultrasonic time,temperature and liquid-to-solid ratio had significant effects on the extraction amount of flavonoids from L.chinense Sarg.× L.tulipifera L.;there was a significant interaction between any two of the factors,which had a significant effect on the extraction of flavonoids;and the optimum extraction parameters for flavonoids in the bark were the ultrasonic time of 33.66 min,the ultrasonic temperature at 32.85 ℃ and the liquid-to-solid ratio of 11.39 ml/g.Ultrasonic time and liquid-to-solid ratio had significant effects on the extraction of flavonoids from roots;there was a significant interaction between ultrasonic time and liquid-to-solid ratio and between ultrasonic temperature and liquid-to-solid ratio;and the optimum extraction parameters for flavonoids from the roots of L.chinense Sarg.× L.tulipifera L.were the ultrasonic time of 32.38 min,the ultrasonic temperature at 25.0 ℃ and the liquid-to-solid ratio of 8.00 ml/g.The results of the three models were stable.The models are feasible and have good application value. 展开更多
关键词 Response surface METHODOLOGY LIRIODENDRON chinense sarg L. tulipifera L. TOTAL FLAVONOIDS ULTRASONIC extraction
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软轴传动山核桃采摘器的设计 被引量:1
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作者 彭樟林 赵旭飞 +1 位作者 陆军 张俊涛 《湖北农业科学》 2015年第13期3261-3264,共4页
为了降低劳动强度并适应工况复杂的采摘环境,采用软轴传动的方式设计了一种便携式山核桃(Carya cathayensis Sarg.)采摘器,该采摘器由采摘头、采摘杆、软轴(动力传递)、动力输出装置构成。动力装置使用齿轮传动,用脚踏的方式进行力的输... 为了降低劳动强度并适应工况复杂的采摘环境,采用软轴传动的方式设计了一种便携式山核桃(Carya cathayensis Sarg.)采摘器,该采摘器由采摘头、采摘杆、软轴(动力传递)、动力输出装置构成。动力装置使用齿轮传动,用脚踏的方式进行力的输出;采摘头连接在软轴的末端,使用软性材料通过旋转击打山核桃果实使其脱落。结果表明,软轴传动能够有效地将脚踏的动力通过齿轮传动转化为采摘头的动力,该采摘器具有采摘方便、移动轻巧、经济高效的特点。 展开更多
关键词 山核桃(Carya cathayensis sarg.) 软轴传动 采摘器
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基于种子发育过程RNA-Seq的山核桃SSR位点分析 被引量:1
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作者 赵国淼 曾燕如 +4 位作者 黄有军 李娟 贾宁 徐亚楠 唐研耀 《分子植物育种》 CAS CSCD 北大核心 2015年第11期2516-2521,共6页
本研究基于山核桃种子发育过程胚和胚乳转录组测序数据,采用MISA软件检测从中所得110 959条Unigene中的SSR位点,并与发育雌花芽中所得的SSR位点数据进行了比较,旨在为今后山核桃多态SSR标记的筛选、开发与应用提供借鉴。分析发现,种子RN... 本研究基于山核桃种子发育过程胚和胚乳转录组测序数据,采用MISA软件检测从中所得110 959条Unigene中的SSR位点,并与发育雌花芽中所得的SSR位点数据进行了比较,旨在为今后山核桃多态SSR标记的筛选、开发与应用提供借鉴。分析发现,种子RNA-Seq数据中含有SSR位点的Unigene占23.76%,其中单核苷酸(Mononucleotide,59.62%)和二核苷酸(Dinucleotide,32.07%)重复是山核桃中主要的SSR重复类型;SSR重复单元的重复次数分布在5~24次之间,其中5~9次重复SSR位点有11 862个,占总数的36.62%;10~14次重复的SSR位点有15 286个,占总数的47.19%;获得了11 883对可供筛选的SSR引物;并发现同一物种不同器官SSR的分布是有所差别的。 展开更多
关键词 山核桃 CARYA cathayensis sarg 转录组测序 SSR
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FTIR and Thermogravimetric Analysis of Three Kinds of Nutshells 被引量:1
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作者 Jinping Zhang Yue Ying +1 位作者 Xuebin Li Xiaohua Yao 《Natural Resources》 2018年第8期313-325,共13页
The main components and pyrolysis characteristics of Camellia oleifera Abel hells, Castanea mollissima Blume shells, and Castanea mollissima Blume shells were analyzed by using FTIR and thermogravimetric methods. The ... The main components and pyrolysis characteristics of Camellia oleifera Abel hells, Castanea mollissima Blume shells, and Castanea mollissima Blume shells were analyzed by using FTIR and thermogravimetric methods. The experimental results indicated that the main components of the three kinds of raw materials consisted of cellulose, hemicellulose, and lignin. The highest contents of hemicellulose, cellulose, and lignin were in Camellia oleiferaAbel shells (49.34% ± 0.07%), Castanea mollissima Blume shells (27.34% ± 0.01%), and Carya cathayensis Sarg shells (49.78% ± 0.01%), respectively. The pyrolysis processes of three kinds of shells generally included three stages, namely dehydration, pyrolysis, and carbonization. The peak values and the appearance times of their pyrolysis rates were closely related to their compositions. 展开更多
关键词 Camellia oleifera ABEL SHELLS CASTANEA mollissima Blume SHELLS CARYA cathayensis sarg SHELLS FTIR THERMOGRAVIMETRIC Analysis Matrix Application
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鹅掌楸叶柄愈伤组织诱导优化 被引量:1
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作者 吕俊琴 刘中兵 陈玲 《湖北农业科学》 北大核心 2014年第8期1933-1935,共3页
以鹅掌楸(Liriodendron chinensis Sarg.)嫩叶叶柄为外植体,优化了愈伤组织诱导条件。结果表明,叶柄经1 g/L的HgCl2溶液处理9 min,接种至添加2 g/L 50%多菌灵的MS培养基中培养20 d时,成活率最高,达58.8%;愈伤组织诱导的最佳培养基是1/2 ... 以鹅掌楸(Liriodendron chinensis Sarg.)嫩叶叶柄为外植体,优化了愈伤组织诱导条件。结果表明,叶柄经1 g/L的HgCl2溶液处理9 min,接种至添加2 g/L 50%多菌灵的MS培养基中培养20 d时,成活率最高,达58.8%;愈伤组织诱导的最佳培养基是1/2 MS+0.2 mg/L 2,4-D+2.0 mg/L 6-BA+0.15 mg/L NAA,诱导率达74.37%。 展开更多
关键词 鹅掌楸(Liriodendron CHINENSIS sarg ) 叶柄 正交试验 灭菌 愈伤组织诱导
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ARGs-OAP v3.0:Antibiotic-Resistance Gene Database Curation and Analysis Pipeline Optimization 被引量:2
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作者 Xiaole Yin Xiawan Zheng +3 位作者 Liguan Li An-Ni Zhang Xiao-Tao Jiang Tong Zhang 《Engineering》 SCIE EI CAS CSCD 2023年第8期234-241,共8页
Antibiotic resistance,which is encoded by antibiotic-resistance genes(ARGs),has proliferated to become a growing threat to public health around the world.With technical advances,especially in the popularization of met... Antibiotic resistance,which is encoded by antibiotic-resistance genes(ARGs),has proliferated to become a growing threat to public health around the world.With technical advances,especially in the popularization of metagenomic sequencing,scientists have gained the ability to decipher the profiles of ARGs in diverse samples with high accuracy at an accelerated speed.To analyze thousands of ARGs in a highthroughput way,standardized and integrated pipelines are needed.The new version(v3.0)of the widely used ARGs online analysis pipeline(ARGs-OAP)has made significant improvements to both the reference database-the structured ARG(SARG)database-and the integrated analysis pipeline.SARG has been enhanced with sequence curation to improve annotation reliability,incorporate emerging resistance genotypes,and determine rigorous mechanism classification.The database has been further organized and visualized online in the format of a tree-like structure with a dictionary.It has also been divided into sub-databases for different application scenarios.In addition,the ARGs-OAP has been improved with adjusted quantification methods,simplified tool implementation,and multiple functions with userdefined reference databases.Moreover,the online platform now provides a diverse biostatistical analysis workflow with visualization packages for the efficient interpretation of ARG profiles.The ARGs-OAP v3.0 with an improved database and analysis pipeline will benefit academia,governmental management,and consultation regarding risk assessment of the environmental prevalence of ARGs. 展开更多
关键词 sarg database ARGs-OAP Antibiotic-resistance genes Environmental metagenome Quantification
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Means-End Chain Based Brand Positioning Strategy for Lin'an Carya Cathayensis Sarg
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作者 Lingyan GAO Yueli ZHANG 《Asian Agricultural Research》 2014年第10期53-56,60,共5页
Based on the theory of Means-End Chain,this paper surveyed value cognition structure of 36 member consumers through the laddering interview. According to the survey,it made an in-depth analysis on brand positioning st... Based on the theory of Means-End Chain,this paper surveyed value cognition structure of 36 member consumers through the laddering interview. According to the survey,it made an in-depth analysis on brand positioning strategy for Lin'an Carya Cathayensis Sarg,and obtained the existing brand operating model. It is hoped to explore effective strategy of brand positioning for agricultural product enterprises in Zhejiang Province,and accordingly lift core competitiveness of agricultural products of Zhejiang Province. 展开更多
关键词 Lin’an CARYA Cathayensis sarg Means-End CHAIN Agri
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Antioxidation effect of Carya cathayensis sarg oil
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作者 Li Zhule Xu Yingbi Chen Honghong 《Agricultural Science & Technology》 CAS 2007年第2期8-12,共5页
[Objective] The antioxidation effect of Carya cathayensis sarg (CCS) Oil was qualitatively and quantitatively detected. [Method] 50 male mice were divided into 5 groups equally and randomly and among them,the three gr... [Objective] The antioxidation effect of Carya cathayensis sarg (CCS) Oil was qualitatively and quantitatively detected. [Method] 50 male mice were divided into 5 groups equally and randomly and among them,the three groups were either negative,or model control,or positive control (ginseng saponins),respectively. Two remaining groups were orally fed with low and high dosages of CCS oil ((9 ml/(kg·d) and (33 ml/(kg·d)),respectively. All groups were injected D-galactose through hypodermic except the negative control ones which were only treated with equals amount of distilled water. The superoxide dismutase (SOD),maleic dialdehyde(MDA) and monoamine oxidase(MAO) were analyzed with blood sample and brain tissue 42 days post treatment. [Result] It was showed that the activity of SOD enzyme was increased in the CCS oil groups,while MDA and MAO content were decreased in the CCS oil groups compared with that of other groups with significance observed in the group fed with high dose of CCS oil. [Conclusion] The result indicated that the CCS oil has the ability of antioxidation and is useful to human health. 展开更多
关键词 MDA MAO T-SOD Carya cathayensis sarg (CCS) oil
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Photosynthetic Characteristic Variation of Different Provenances of Liriodendron chinense Sarg. at Seedling Stage
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作者 惠利省 徐立安 王章荣 《Agricultural Science & Technology》 CAS 2010年第1期122-125,共4页
[Objective]The aim was to study photosynthetic characteristic variation of different provenances of Liriodendron chinense Sarg. at seedling stage and provide the theoretical basis for work of provenance selection,pare... [Objective]The aim was to study photosynthetic characteristic variation of different provenances of Liriodendron chinense Sarg. at seedling stage and provide the theoretical basis for work of provenance selection,parent selection of hybridizing breeding,genetic resource conservation of L.chinense Sarg.. [Method]With 13 provenances 1-year-old L.chinense Sarg. seedlings as tested materials,the photosynthetic characteristic variation of different provenances were studied. [Result]The L.chinense Sarg. seedlings of different provenances had the significant difference on net photosynthetic rate (Pn),stomata conductivity (Gs) and water use efficiency (WUE). The Pn of the highest provenance was 2.36 times the lowest. Majority seed sources light saturation point (LSP) were in 800-1 200 μmol photons/(m2·s). Gs,transpiration rate (Tr) and Pn were significantly positively correlated,and the three had a significant positive correlation with the light compensation point (LCP). Pn,LSP and WUE were negatively correlated. [Conclusion]The photosynthetic characteristics of L.chinense Sarg. had significant variation among provenances. Maybe Gs is the important factor leading to difference in Pn of L.chinense Sarg. seedlings. L.chinense Sarg. seedlings still need higher light intensity and more water. 展开更多
关键词 Liriodendron chinense sarg. PROVENANCE Photosynthetic characteristics
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Linux环境中代理服务器的配置与应用
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作者 周莉 谭方勇 《电脑知识与技术》 2015年第8期13-15,共3页
该文Linux中代理服务器squid为研究对象,分析代理服务器工作原理,阐述squid代理服务器的具体工作过程,给出了基于Linux iptables防火墙和squid代理服务器的透明代理的搭建方法,说明了squid的日志管理的具体步骤。
关键词 代理服务器 透明代理 SQUID IPTABLES sarg
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山核桃仁中主要营养成分的研究 被引量:94
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作者 王冀平 李亚南 马建伟 《食品科学》 EI CAS CSCD 北大核心 1998年第4期44-46,共3页
用氨基酸分析仪和气相色谱仪及经典的化学分析法对山核桃仁中的氨基酸、脂肪酸、蛋白质、脂肪的组成和含量的测定,共测出17种氨基酸,8种脂肪酸,还对山核桃和胡桃的上述成分进行了营养价值和保健作用的对比,认为山核桃比胡桃更有独... 用氨基酸分析仪和气相色谱仪及经典的化学分析法对山核桃仁中的氨基酸、脂肪酸、蛋白质、脂肪的组成和含量的测定,共测出17种氨基酸,8种脂肪酸,还对山核桃和胡桃的上述成分进行了营养价值和保健作用的对比,认为山核桃比胡桃更有独特的优点。 展开更多
关键词 脂肪酸 蛋白质 氨基酸 山核桃仁
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杂种鹅掌楸扦插生根过程中内源激素的变化 被引量:57
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作者 张晓平 方炎明 黄绍辉 《南京林业大学学报(自然科学版)》 CAS CSCD 北大核心 2004年第3期79-82,共4页
以杂种鹅掌楸为材料,应用酶联免疫吸附测定法(ELISA),研究杂种鹅掌楸插条在扦插生根过程中内源激素含量的变化。结果表明:在不定根发生过程中,5种内源激素在插穗基部和叶内含量总体呈上升趋势,少数下降。各种内源激素在叶内的含量远远... 以杂种鹅掌楸为材料,应用酶联免疫吸附测定法(ELISA),研究杂种鹅掌楸插条在扦插生根过程中内源激素含量的变化。结果表明:在不定根发生过程中,5种内源激素在插穗基部和叶内含量总体呈上升趋势,少数下降。各种内源激素在叶内的含量远远高于插穗基部。外源激素(300mg/kgNAA和100mg/kgIBA)处理提高了iPAs和IAA在插穗基部和叶内的含量,却降低了ZRs和ABA的含量,对GA1/3的作用有些不稳定。杂种鹅掌楸插穗内IAA的含量很低,而插穗和叶内ABA的含量却很高,这对不定根的发生很不利。 展开更多
关键词 杂种鹅掌楸 扦插 内源激素
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利用RAPD标记分析北美鹅掌楸与鹅掌楸种间遗传多样性 被引量:44
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作者 罗光佐 施季森 +2 位作者 尹佟明 黄敏仁 王明庥 《植物资源与环境学报》 CAS CSCD 2000年第2期9-13,共5页
利用RAPD标记技术对鹅掌楸属 (LiriodendronL .) 2个现存种鹅掌楸〔Liriodendronchinense (Hemsl.)Sarg.〕和北美鹅掌楸 (LiriodendrontulipiferaLinn .)的遗传多样性进行分析 ,结果表明 :2个种都有较高的遗传多样性 ,但北美鹅掌楸的遗... 利用RAPD标记技术对鹅掌楸属 (LiriodendronL .) 2个现存种鹅掌楸〔Liriodendronchinense (Hemsl.)Sarg.〕和北美鹅掌楸 (LiriodendrontulipiferaLinn .)的遗传多样性进行分析 ,结果表明 :2个种都有较高的遗传多样性 ,但北美鹅掌楸的遗传多样性水平高于鹅掌楸 ;鹅掌楸的遗传变异主要来自地理种源内 ,而北美鹅掌楸的遗传变异主要来自地理种源间 ;与美国密苏里和路易斯安娜的北美鹅掌楸相比 ,鹅掌楸的遗传距离更接近于南卡罗来那。 展开更多
关键词 北美鹅掌楸 遗传多样性 RAPD 分子标记
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杂种鹅掌楸插穗不定根发生与发育的解剖学观察 被引量:45
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作者 张晓平 方炎明 《植物资源与环境学报》 CAS CSCD 2003年第1期10-15,共6页
从解剖学角度着手,对杂种鹅掌楸〔Liriodendronchinense(Hemsl.)Sarg.×L.tulipiferaL.〕扦插过程中不定根的发生发育进行了研究。结果表明:杂种鹅掌楸插穗内未发现潜伏根原基。扦插后,不定根原基起源于维管形成层区,属于诱导生根... 从解剖学角度着手,对杂种鹅掌楸〔Liriodendronchinense(Hemsl.)Sarg.×L.tulipiferaL.〕扦插过程中不定根的发生发育进行了研究。结果表明:杂种鹅掌楸插穗内未发现潜伏根原基。扦插后,不定根原基起源于维管形成层区,属于诱导生根类型。维管形成层恢复活动后,在不定根发生的部位附近形成1个明显的多薄壁细胞区域,在此区域不定根较容易发生。愈伤组织内没有发现根原基,愈伤组织在发育的过程中,内部细胞部分分化,并形成不规则的输导组织。大量的愈伤组织对不定根的发生有较强的抑制作用。杂种鹅掌楸插穗上不定根的发生可分为4个阶段:(1)维管形成层恢复活动,分裂出多层薄壁细胞;(2)维管形成层及附近的薄壁细胞脱分化,形成不定根原基发端细胞;(3)根原基发端细胞不断分裂成具有方向性的根原基,根原基穿过韧皮射线和皮层,向皮孔或下切口方向发展;(4)不定根从皮孔或下切口伸出,其内部的维管系统开始发育。 展开更多
关键词 杂种鹅掌楸 插穗 不定根 发生 发育 解剖学
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鹅掌楸花粉败育过程的超微结构观察 被引量:34
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作者 尹增芳 樊汝汶 《植物资源与环境》 CSCD 1997年第2期1-7,共7页
花粉败育是限制鹅掌楸〔Liriodendronchinense(Hemsl.)Sarg.〕生殖成功的重要因素之一。败育多数发生在四分体形成之前,少数发生在小孢子形成以后,是由于花粉发育过程中存在异常现象造成的。异常现... 花粉败育是限制鹅掌楸〔Liriodendronchinense(Hemsl.)Sarg.〕生殖成功的重要因素之一。败育多数发生在四分体形成之前,少数发生在小孢子形成以后,是由于花粉发育过程中存在异常现象造成的。异常现象有7个方面:(1)造孢组织解体;(2)小孢子形成过程中胼胝质的积累与降解异常;(3)绒毡层发育异常;(4)小孢子母细胞胞质分裂异常;(5)小孢子解体;(6)生殖细胞败育;(7)药隔维管束韧皮部的伴胞解体。这些原因可引起花粉产量和质量降低。 展开更多
关键词 鹅掌楸 花粉 败育 生殖 超微结构
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浙江临安山核桃立地环境研究 被引量:39
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作者 吕惠进 《森林工程》 2005年第1期1-3,6,共4页
我国山核桃(Carya cathayensis Sarg.)具有易栽培、花工少、经济效益高等特点,主要分布在浙西皖南地区,但其适生环境比较独特,在临安市具有很强的地域分布特征。通过对该市山核桃主要产区的立地环境调查,分析了山核桃生长适宜的由地质... 我国山核桃(Carya cathayensis Sarg.)具有易栽培、花工少、经济效益高等特点,主要分布在浙西皖南地区,但其适生环境比较独特,在临安市具有很强的地域分布特征。通过对该市山核桃主要产区的立地环境调查,分析了山核桃生长适宜的由地质、地貌、土壤、气候等因素构成的立地环境,为当地低产林的改造、规模种植和异地引种提供依据。 展开更多
关键词 山核桃 立地环境 临安市
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山核桃提取物体外抗肿瘤作用研究 被引量:43
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作者 胡旭姣 赵肖君 +5 位作者 周奋 余美荣 陈银银 李瑞枝 贾永慧 丁志山 《中华中医药学刊》 CAS 2007年第2期369-370,共2页
目的:探讨山核桃的抗肿瘤作用。方法:采用极性溶剂法从山核桃叶子中分离提取出石油醚、氯仿、乙酸乙酯、正丁醇和水提取物5种提取物,采用MTT法观察其对肿瘤细胞SGC-7901增殖的抑制作用,并采用光学显微镜和电镜方法观察乙酸乙酯提取物对S... 目的:探讨山核桃的抗肿瘤作用。方法:采用极性溶剂法从山核桃叶子中分离提取出石油醚、氯仿、乙酸乙酯、正丁醇和水提取物5种提取物,采用MTT法观察其对肿瘤细胞SGC-7901增殖的抑制作用,并采用光学显微镜和电镜方法观察乙酸乙酯提取物对SGC-7901细胞凋亡的诱导作用。结果:山核桃乙酸乙酯提取物抗肿瘤作用最强,并且能够诱导肿瘤细胞凋亡。结论:山核桃叶子中有抗肿瘤物质,值得进一步深入研究。 展开更多
关键词 山核桃 抗肿瘤 乙酸乙酯提取物 凋亡
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鹅掌楸(Liriodendron chinense)遗传多样性的等位酶论证 被引量:26
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作者 朱晓琴 马建霞 +2 位作者 姚青菊 郝日明 贺善安 《植物资源与环境》 CSCD 1995年第3期9-14,共6页
选择5个具有地理代表性的鹅掌楸(Liriodendronchinense(Hemsl.)Sarg.)种群,分析6种酶系统9个位点上27个等位基因的遗传变异。结果表明,鹅掌楸种内保存较高的等位酶变异。遗传多样性的分布特... 选择5个具有地理代表性的鹅掌楸(Liriodendronchinense(Hemsl.)Sarg.)种群,分析6种酶系统9个位点上27个等位基因的遗传变异。结果表明,鹅掌楸种内保存较高的等位酶变异。遗传多样性的分布特点与种群地理分布型式一致,鹅掌楸的种内分化主要表现为东、西部种群之间的差异,东部亚区的总的基因丰富程度低于西部亚区。同时,东部亚区内“岛”状隔离分布使其中的种群分化加深,种群间差异大于西部亚区中呈“带”状地理连续分布的种群间的分化。 展开更多
关键词 鹅掌楸 等位酶 遗传多样性 种群分化
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