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九个水稻耐盐突变体的RFLP分析 被引量:11
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作者 张耕耘 郭岩 +2 位作者 刘凤华 陈受宜 陈少麟 《Acta Botanica Sinica》 CSCD 1994年第5期345-350,共6页
用6 个可能与水稻耐盐性有关的DNA 探针对来自两个品系的5 个耐盐突变株系、3 个耐盐突变体及1 个弱耐盐突变体进行RFLP分析。结果有8 个耐盐突变体(株系)检测到DNA 水平的变化,6 个探针检测到具多态性的突变株系(... 用6 个可能与水稻耐盐性有关的DNA 探针对来自两个品系的5 个耐盐突变株系、3 个耐盐突变体及1 个弱耐盐突变体进行RFLP分析。结果有8 个耐盐突变体(株系)检测到DNA 水平的变化,6 个探针检测到具多态性的突变株系(体)的数目分别为RG4:6 个;RG711:6 个;Rab16:5 个;Rab10E6:2 个;Rab21:1 个;SalT:2 个;表明RG4、RG711 及Rab16三个位点有可能与耐盐性突变相关。多态性图谱中70.8% 为2 个以上的酶切图谱同时显示多态性,说明多数突变是由缺失。 展开更多
关键词 水稻 耐盐突变体 RFLP 分析
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RAPD tagging of a salt tolerant gene in rice 被引量:2
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作者 Ding Haiyuan Zhang Gengyun +2 位作者 Guo Yan Chen Shaolin Chen Shouyi 《Chinese Science Bulletin》 SCIE EI CAS 1998年第4期330-332,共3页
A salt tolerant rice mutant was obtained through tissue culture. The inheritance of salt tolerance in F2 population of mutant crossed with its original variety under salt stress was studied. According to the standard,... A salt tolerant rice mutant was obtained through tissue culture. The inheritance of salt tolerance in F2 population of mutant crossed with its original variety under salt stress was studied. According to the standard, the ratio of salt tolerant: sensitive was about 3:1 suggesting that there was a major gene related to salt tolerance in the mutant. By RAPD analysis with 220 10mer primers, the gene was tagged by a 1.0 kb DNA fragment named OPS1210 which was located on chromosome 7. The genetic distance between the major salt tolerant gene was 16.4 cm. 展开更多
关键词 RICE salt tolerant mutant line RAPD MOLECULAR marker.
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