Ablotlc stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very Important role In plants...Ablotlc stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very Important role In plants. To characterize the catalase involved In plant response to ablotlc stresses, we constructed a cDNA library from 4℃-treated Festuca arundinacea Schreb seedlings and isolated a catalase gene from this library. The cDNA （FaCat1, 1 735 bp） contained an open reading frame of 1 479 bp. BLAST analysis Indicated that the deduced amino acid sequence showed 96% Identity with that from wheat TaCat1 and 87% Identity with that from maize ZmCat2. Northern blotting analysis showed an obvious Increase of FaCat1 transcripts In leaves In contrast with roots. Time-course analysis of the expression of FaCat1 in F. arundinacea leaves showed that FaCat1 expression was upregulated in cold- and salt-stressed leaves, with the FaCat1 transcripts accumulat-Ing mostly at 4 or 2 h after cold or salt stress, respectively. No significant changes in FaCat1 transcription were observed in dried leaves and inhibition of FaCat1 transcription was found In absclsic acid （ABA）-treated leaves, Indicating that the FaCat1 gene is differentially expressed during cold, high salt, drought, and ABA treatment In F. arundinacea leaves.展开更多
在土培条件下采用模拟盐胁迫的方法,对16个辣椒(Capsicum annum L.)品种在盐胁迫下萌芽期和幼苗期的生长和生理特性进行研究。结果表明,在盐胁迫下所有供试辣椒品种在萌芽期的发芽率、发芽势、发芽指数、苗长、胚根长、苗重和胚根重均...在土培条件下采用模拟盐胁迫的方法,对16个辣椒(Capsicum annum L.)品种在盐胁迫下萌芽期和幼苗期的生长和生理特性进行研究。结果表明,在盐胁迫下所有供试辣椒品种在萌芽期的发芽率、发芽势、发芽指数、苗长、胚根长、苗重和胚根重均有不同程度的降低;幼苗期的过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性总体表现为增加,可溶性糖和可溶性蛋白含量则总体呈现下降趋势,超氧化物歧化酶(SOD)不同品种表现不同。多元方差分析表明,相对发芽率、相对发芽指数等14个性状指标表现出显著差异性,以隶属函数值为依据的系统聚类分析将供试的辣椒品种分为3类,其中红艳、剑圣1、万家灯火、长虹361、红秀和北研尖椒3号为耐盐性较强的品种,剑圣2、博辣五号、红圣008、剑圣3和苏椒12号为耐盐性中等品种,利剑1、干鲜三号、长虹366、艳红1和艳红2为耐盐性较弱品种。展开更多
Salt tolerance of crops is becoming more and more important, owing to the constant increase of salinity in arid and semi-arid regions. Broomcorn millet(Panicum miliaceum L.),generally considered tolerant to salinity, ...Salt tolerance of crops is becoming more and more important, owing to the constant increase of salinity in arid and semi-arid regions. Broomcorn millet(Panicum miliaceum L.),generally considered tolerant to salinity, can be an alternative crop for salt affected areas.To assess genotypic variation for vegetative-stage salinity tolerance, 195 broomcorn millet accessions from a core collection were evaluated for germination percentage, shoot length,and root length during germination in 8 m L of deionized water(control) or 8 m L of a120 mmol L-1salt solution(treatment). Six genotypes with different levels of salt tolerance were selected based on the growth parameters and ion concentrations in plant at the seedling stage and used for confirmation of the initial salinity response. Substantial variation for salinity tolerance was found on the basis of salt damage index [(germination percentage under control- germination percentage under salinity) / germination percentage under control × 100, SDI] and 39 accessions exhibited strong salt tolerance with SDI lower than 20%. The salt tolerance performance of the genotypes was generally consistent across experiments. In the seedling growth study, seedling number, root length and belowground biomass were adversely affected(showing more than 70%, 50%, and 32%reduction, respectively) in sensitive genotypes compared to tolerant genotypes(35%, 31%,and 3% reduction, respectively) under 160 mmol L-1Na Cl treatment. In general,whole-plant salinity tolerance was associated with increased Na+concentration and Na+/K+ratio, and salt-tolerant genotypes often had higher root and lower shoot Na+concentration than sensitive ones. Na+concentration in root was closely related to salt tolerance and may be considered as a selection criterion for screening salt tolerance of broomcorn millet at the seedling or vegetative stages.展开更多
文摘Ablotlc stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very Important role In plants. To characterize the catalase involved In plant response to ablotlc stresses, we constructed a cDNA library from 4℃-treated Festuca arundinacea Schreb seedlings and isolated a catalase gene from this library. The cDNA （FaCat1, 1 735 bp） contained an open reading frame of 1 479 bp. BLAST analysis Indicated that the deduced amino acid sequence showed 96% Identity with that from wheat TaCat1 and 87% Identity with that from maize ZmCat2. Northern blotting analysis showed an obvious Increase of FaCat1 transcripts In leaves In contrast with roots. Time-course analysis of the expression of FaCat1 in F. arundinacea leaves showed that FaCat1 expression was upregulated in cold- and salt-stressed leaves, with the FaCat1 transcripts accumulat-Ing mostly at 4 or 2 h after cold or salt stress, respectively. No significant changes in FaCat1 transcription were observed in dried leaves and inhibition of FaCat1 transcription was found In absclsic acid （ABA）-treated leaves, Indicating that the FaCat1 gene is differentially expressed during cold, high salt, drought, and ABA treatment In F. arundinacea leaves.
文摘在土培条件下采用模拟盐胁迫的方法,对16个辣椒(Capsicum annum L.)品种在盐胁迫下萌芽期和幼苗期的生长和生理特性进行研究。结果表明,在盐胁迫下所有供试辣椒品种在萌芽期的发芽率、发芽势、发芽指数、苗长、胚根长、苗重和胚根重均有不同程度的降低;幼苗期的过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性总体表现为增加,可溶性糖和可溶性蛋白含量则总体呈现下降趋势,超氧化物歧化酶(SOD)不同品种表现不同。多元方差分析表明,相对发芽率、相对发芽指数等14个性状指标表现出显著差异性,以隶属函数值为依据的系统聚类分析将供试的辣椒品种分为3类,其中红艳、剑圣1、万家灯火、长虹361、红秀和北研尖椒3号为耐盐性较强的品种,剑圣2、博辣五号、红圣008、剑圣3和苏椒12号为耐盐性中等品种,利剑1、干鲜三号、长虹366、艳红1和艳红2为耐盐性较弱品种。
基金supported by the National Millet Crops Research and Development System (CARS-07-12[1].5-A1)the National Key Technology R&D Program of China (2013BAD01B05-2)
文摘Salt tolerance of crops is becoming more and more important, owing to the constant increase of salinity in arid and semi-arid regions. Broomcorn millet(Panicum miliaceum L.),generally considered tolerant to salinity, can be an alternative crop for salt affected areas.To assess genotypic variation for vegetative-stage salinity tolerance, 195 broomcorn millet accessions from a core collection were evaluated for germination percentage, shoot length,and root length during germination in 8 m L of deionized water(control) or 8 m L of a120 mmol L-1salt solution(treatment). Six genotypes with different levels of salt tolerance were selected based on the growth parameters and ion concentrations in plant at the seedling stage and used for confirmation of the initial salinity response. Substantial variation for salinity tolerance was found on the basis of salt damage index [(germination percentage under control- germination percentage under salinity) / germination percentage under control × 100, SDI] and 39 accessions exhibited strong salt tolerance with SDI lower than 20%. The salt tolerance performance of the genotypes was generally consistent across experiments. In the seedling growth study, seedling number, root length and belowground biomass were adversely affected(showing more than 70%, 50%, and 32%reduction, respectively) in sensitive genotypes compared to tolerant genotypes(35%, 31%,and 3% reduction, respectively) under 160 mmol L-1Na Cl treatment. In general,whole-plant salinity tolerance was associated with increased Na+concentration and Na+/K+ratio, and salt-tolerant genotypes often had higher root and lower shoot Na+concentration than sensitive ones. Na+concentration in root was closely related to salt tolerance and may be considered as a selection criterion for screening salt tolerance of broomcorn millet at the seedling or vegetative stages.
