A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot ...A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘9311’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana).展开更多
由土传真菌稻粒黑粉菌引起的稻粒黑粉病是水稻杂交制种田中的主要病害之一。为初步解析稻粒黑粉病菌侵染过程中与寄主的互作分子机制,本研究用稻粒黑粉病菌强毒力菌株JY-521侵染感病不育系材料“9311A”,于侵染8、12、24、48和72 h 5个...由土传真菌稻粒黑粉菌引起的稻粒黑粉病是水稻杂交制种田中的主要病害之一。为初步解析稻粒黑粉病菌侵染过程中与寄主的互作分子机制,本研究用稻粒黑粉病菌强毒力菌株JY-521侵染感病不育系材料“9311A”,于侵染8、12、24、48和72 h 5个时间点取菌样进行转录组测序分析。结果表明,与未侵染对照组相比,在侵染8 h时,差异表达基因(DEGs)数最多,有337个DEGs;其中上调的DEGs有120个,下调的DEGs有217个。337个DEGs的KEGG富集分析表明,脂肪酸代谢和过氧化物酶体可能是稻粒黑粉病菌侵染过程中的关键代谢途径。此外,对编码碳水化合物酶、分泌蛋白和宿主植物—病原互作蛋白的DEGs在5个侵染时间点的表达模式进行解析,结果表明,一些显著上调表达的DEGs可能是稻粒黑粉病菌侵染的关键致病因子。该研究结果为解析稻粒黑粉病菌的致病分子机制,进一步防控该病害奠定了理论基础。展开更多
基金the National High-Tech Research and Development Project (863) (Grant No. 101-01-02-02)National Specific Program for Research+1 种基金Industrialization of Transgenic Plant (Grant Nos. J00-A-009 and J99-B-012),Science & Technology Project of Guangdong Province (Grant No. B201)
文摘A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of ‘9311’, an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four anti-fungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of ‘9311’, co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F1 plants, resulting from a cross of R2 homo-zygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana).
文摘由土传真菌稻粒黑粉菌引起的稻粒黑粉病是水稻杂交制种田中的主要病害之一。为初步解析稻粒黑粉病菌侵染过程中与寄主的互作分子机制,本研究用稻粒黑粉病菌强毒力菌株JY-521侵染感病不育系材料“9311A”,于侵染8、12、24、48和72 h 5个时间点取菌样进行转录组测序分析。结果表明,与未侵染对照组相比,在侵染8 h时,差异表达基因(DEGs)数最多,有337个DEGs;其中上调的DEGs有120个,下调的DEGs有217个。337个DEGs的KEGG富集分析表明,脂肪酸代谢和过氧化物酶体可能是稻粒黑粉病菌侵染过程中的关键代谢途径。此外,对编码碳水化合物酶、分泌蛋白和宿主植物—病原互作蛋白的DEGs在5个侵染时间点的表达模式进行解析,结果表明,一些显著上调表达的DEGs可能是稻粒黑粉病菌侵染的关键致病因子。该研究结果为解析稻粒黑粉病菌的致病分子机制,进一步防控该病害奠定了理论基础。
