目的研究飞行员血液视黄醇结合蛋白4(retinol binding protein 4,RBP4)水平变化及其与冠心病危险因素的相关性。方法选取军事飞行员477例,冠心病患者44例,普通地面工作健康体检者202例,采用酶联免疫吸附法测定受试者R BP4水平,测量身高...目的研究飞行员血液视黄醇结合蛋白4(retinol binding protein 4,RBP4)水平变化及其与冠心病危险因素的相关性。方法选取军事飞行员477例,冠心病患者44例,普通地面工作健康体检者202例,采用酶联免疫吸附法测定受试者R BP4水平,测量身高、体重、血压等,并采集记录相应生化数据:总胆固醇(TC)、三酰甘油(TG)、空腹血糖(FBG)、尿酸(UA),应用统计软件进行统计检验。结果冠心病组血液RBP4水平明显高于普通健康人群组(t=5.986,P<0.01);飞行员组血液RBP4水平明显高于普通健康人群组(t=4.822,P<0.01)。在相同年龄组中,飞行员组血液RBP4水平均高于普通健康人群组(t=2.091、3.048、3.424,P<0.05),但飞行员各年龄组间血液RBP4水平差异无统计学意义;飞行员组不同飞行时间组间血液RBP4水平差异无统计学意义。结论飞行因素可能导致飞行员血液RBP4水平升高,而RBP4水平升高可能是飞行员冠心病的危险因素。展开更多
目的:观察肿瘤坏死因子-α(TNF-α)对体外培养的成熟3T3-L1脂肪细胞分泌视黄醛结合蛋白4(Retinol Binding Protein 4,RBP4)的影响。方法:体外培养3T3-L1细胞,待细胞完全融合后两天进行诱导分化为成熟脂肪细胞,应用不同浓度的重组TNF-α...目的:观察肿瘤坏死因子-α(TNF-α)对体外培养的成熟3T3-L1脂肪细胞分泌视黄醛结合蛋白4(Retinol Binding Protein 4,RBP4)的影响。方法:体外培养3T3-L1细胞,待细胞完全融合后两天进行诱导分化为成熟脂肪细胞,应用不同浓度的重组TNF-α干预,采用放射免疫分析法检测干预后不同时间成熟脂肪细胞RBP4的分泌量。结果:(1)不同浓度TNF-α(0.2、1.0、5.0、10ng/ml)对脂肪细胞RBP4的分泌均有抑制作用,其抑制效应呈剂量依赖性:随着TNF-α浓度的增加,RBP4水平降低越加明显。(2)各实验组随着干预时间延长,RBP4虽有降低之趋势,但组内各时间点差异无统计学差异。(3)二元回归分析提示TNF-α对RBP4有影响。结论:TNF-α能抑制成熟脂肪细胞RBP4的分泌,其抑制效应有剂量依赖性,而与干预时间无关。展开更多
目的:探究血清甘油三酯-葡萄糖(TyG)指数、摄食抑制因子-1(nesfatin-1)、视黄醇结合蛋白4(RBP4)联合预测糖尿病视网膜病变(DR)的价值,为DR早期预测提供支持。方法:回顾性分析。收集2022-02/2023-12我院接诊的2型糖尿病(T2DM)患者164例...目的:探究血清甘油三酯-葡萄糖(TyG)指数、摄食抑制因子-1(nesfatin-1)、视黄醇结合蛋白4(RBP4)联合预测糖尿病视网膜病变(DR)的价值,为DR早期预测提供支持。方法:回顾性分析。收集2022-02/2023-12我院接诊的2型糖尿病(T2DM)患者164例的临床资料,按照眼底检查结果分为DR组43例(其中增殖性DR 19例,非增殖性DR 24例),不合并DR的T2DM组121例。入院后记录患者基本资料,检查血清TyG指数、nesfatin-1、RBP4水平。结果:DR组病程长于T2DM组,空腹血糖、糖化血红蛋白、甘油三酯、总胆固醇、低密度脂蛋白及TyG指数、RBP4水平高于T2DM组,高密度脂蛋白、nesfatin-1水平低于T2DM组(均P<0.001)。多因素Logistic回归分析可知,T2DM病程(OR=1.338,95%CI:1.059-1.690)、糖化血红蛋白(OR=5.065,95%CI:1.659-15.470)、低密度脂蛋白(OR=12.715,95%CI:2.385-67.790)、TyG指数(OR=23.057,95%CI:2.936-181.073)、RBP4(OR=1.319,95%CI:1.028-1.692)是T2DM患者发生DR的危险因素,nesfatin-1(OR=0.007,95%CI:0.003-0.016)为保护因素。绘制ROC曲线显示,TyG指数、nesfatin-1、RBP4均对T2DM患者并发DR具有一定预测价值,曲线下面积(areas under curve,AUC)分别为0.804、0.878、0.738,各指标联合预测时AUC为0.946,预测敏感度为83.72%、特异度为92.56%。增殖性DR患者TyG指数、RBP4水平高于非增殖性DR患者,nesfatin-1水平低于非增殖性DR患者(均P<0.05)。Spearman相关性分析显示,TyG指数、RBP4水平与DR病情程度呈正相关,nesfatin-1水平与DR病情程度呈负相关(r_(s)=0.557、0.392、-0.359,均P<0.05)。Pearson相关分析显示,T2DM并发DR患者TyG指数与nesfatin-1水平呈负相关,与RBP4水平呈正相关,nesfatin-1与RBP4水平呈负相关(r=-0.486、0.538、-0.592,均P<0.05)。结论:血清TyG指数、nesfatin-1、RBP4水平与DR发病风险及病情程度有关,可作为DR早期预测的标志物,且联合预测效能更好。展开更多
It has been reported that retinol-binding protein 4(RBP4) is associated to adiposity,insulin resistance,and type 2 diabetes.Meanwhile,circulating RBP4 levels are also affected by renal function.The aim of the present ...It has been reported that retinol-binding protein 4(RBP4) is associated to adiposity,insulin resistance,and type 2 diabetes.Meanwhile,circulating RBP4 levels are also affected by renal function.The aim of the present study is to investigate whether serum levels of RBP4 are primarily associated with different stages of chronic kidney disease(CKD) or type 2 diabetes,if there is more potential relevance between RBP4 and renal replacement therapy.The serum levels of RBP4 were assessed by commercial competitive enzyme-linked immunosorbent assay(ELISA)kit in 212 patients with the CKD stages 1—5 and in 24 healthy controls,while its correlation with clinical and metabolic parameters was analyzed.The serum level of RBP4 had a strong correlation with estimated glomerular filtration rate(e GFR)(P < 0.001).Stratified by e GFR and treatment,no more differences in RBP4 serum concentration were detected between type 2 diabetic and non-diabetic subjects [CKD stages 1—5,non-dialysis(ND),hemodialysis(HD) and peritoneal dialysis(PD);P > 0.05 for all].The elevation of RBP4 become higher in HD than in PD and ND in CKD5 patients(P = 0.008 and P = 0.04,respectively),while there was no significant difference between PD and ND groups.Multivariate linear regression analysis demonstrated three independent predictors of e GFR(β =-0.676,P < 0.001),C-reactive protein(CRP)(β =-0.573,P < 0.001) and creatine(β = 0.509,P = 0.024) in the study population.The study results demonstrated that the serum level of RBP4 was negatively related to the e GFR,whether diabetes mellitus(DM) affected the blood concentration of RBP4 or not.And the serum level of RBP4 exhibited significant difference in different renal replacement therapies.展开更多
目的探讨视黄醇结合蛋白4(RBP4)的蛋白表达量及RBP4 m RNA表达量在胆囊结石合并2型糖尿病组和未合并糖尿病组的胆囊组织中的差异。方法首先应用免疫组化检测30例病例组与30例对照组胆囊组织上RBP4的表达情况,然后用Western blot技术检...目的探讨视黄醇结合蛋白4(RBP4)的蛋白表达量及RBP4 m RNA表达量在胆囊结石合并2型糖尿病组和未合并糖尿病组的胆囊组织中的差异。方法首先应用免疫组化检测30例病例组与30例对照组胆囊组织上RBP4的表达情况,然后用Western blot技术检测胆囊黏膜组织上RBP4的表达量,并与相关临床资料结合进行统计学分析。结果免疫组化检测到RBP4在病例组胆囊组织中的表达强度明显高于对照组(P<0.001),女性表达强度明显高于男性(P<0.001),腹型肥胖者表达强度明显高于非腹型肥胖者(P<0.001);Western blot结果显示:RBP4在病例组的胆囊黏膜组织上的蛋白相对表达量为(2.91±0.86),对照组为(2.17±0.73),差异有统计学意义(P<0.001),病例组RBP4在胆囊黏膜组织中的蛋白表达量明显高于对照组。结论通过免疫组化和Western blot实验,在分子生物学的层面上证明了2型糖尿病组胆囊组织的RBP4表达水平高于对照组,同时女性RBP4的表达强度高于男性,腹型肥胖者RBP4的表达强度高于非腹型肥胖者。展开更多
文摘目的研究飞行员血液视黄醇结合蛋白4(retinol binding protein 4,RBP4)水平变化及其与冠心病危险因素的相关性。方法选取军事飞行员477例,冠心病患者44例,普通地面工作健康体检者202例,采用酶联免疫吸附法测定受试者R BP4水平,测量身高、体重、血压等,并采集记录相应生化数据:总胆固醇(TC)、三酰甘油(TG)、空腹血糖(FBG)、尿酸(UA),应用统计软件进行统计检验。结果冠心病组血液RBP4水平明显高于普通健康人群组(t=5.986,P<0.01);飞行员组血液RBP4水平明显高于普通健康人群组(t=4.822,P<0.01)。在相同年龄组中,飞行员组血液RBP4水平均高于普通健康人群组(t=2.091、3.048、3.424,P<0.05),但飞行员各年龄组间血液RBP4水平差异无统计学意义;飞行员组不同飞行时间组间血液RBP4水平差异无统计学意义。结论飞行因素可能导致飞行员血液RBP4水平升高,而RBP4水平升高可能是飞行员冠心病的危险因素。
文摘目的:观察肿瘤坏死因子-α(TNF-α)对体外培养的成熟3T3-L1脂肪细胞分泌视黄醛结合蛋白4(Retinol Binding Protein 4,RBP4)的影响。方法:体外培养3T3-L1细胞,待细胞完全融合后两天进行诱导分化为成熟脂肪细胞,应用不同浓度的重组TNF-α干预,采用放射免疫分析法检测干预后不同时间成熟脂肪细胞RBP4的分泌量。结果:(1)不同浓度TNF-α(0.2、1.0、5.0、10ng/ml)对脂肪细胞RBP4的分泌均有抑制作用,其抑制效应呈剂量依赖性:随着TNF-α浓度的增加,RBP4水平降低越加明显。(2)各实验组随着干预时间延长,RBP4虽有降低之趋势,但组内各时间点差异无统计学差异。(3)二元回归分析提示TNF-α对RBP4有影响。结论:TNF-α能抑制成熟脂肪细胞RBP4的分泌,其抑制效应有剂量依赖性,而与干预时间无关。
文摘目的:探究血清甘油三酯-葡萄糖(TyG)指数、摄食抑制因子-1(nesfatin-1)、视黄醇结合蛋白4(RBP4)联合预测糖尿病视网膜病变(DR)的价值,为DR早期预测提供支持。方法:回顾性分析。收集2022-02/2023-12我院接诊的2型糖尿病(T2DM)患者164例的临床资料,按照眼底检查结果分为DR组43例(其中增殖性DR 19例,非增殖性DR 24例),不合并DR的T2DM组121例。入院后记录患者基本资料,检查血清TyG指数、nesfatin-1、RBP4水平。结果:DR组病程长于T2DM组,空腹血糖、糖化血红蛋白、甘油三酯、总胆固醇、低密度脂蛋白及TyG指数、RBP4水平高于T2DM组,高密度脂蛋白、nesfatin-1水平低于T2DM组(均P<0.001)。多因素Logistic回归分析可知,T2DM病程(OR=1.338,95%CI:1.059-1.690)、糖化血红蛋白(OR=5.065,95%CI:1.659-15.470)、低密度脂蛋白(OR=12.715,95%CI:2.385-67.790)、TyG指数(OR=23.057,95%CI:2.936-181.073)、RBP4(OR=1.319,95%CI:1.028-1.692)是T2DM患者发生DR的危险因素,nesfatin-1(OR=0.007,95%CI:0.003-0.016)为保护因素。绘制ROC曲线显示,TyG指数、nesfatin-1、RBP4均对T2DM患者并发DR具有一定预测价值,曲线下面积(areas under curve,AUC)分别为0.804、0.878、0.738,各指标联合预测时AUC为0.946,预测敏感度为83.72%、特异度为92.56%。增殖性DR患者TyG指数、RBP4水平高于非增殖性DR患者,nesfatin-1水平低于非增殖性DR患者(均P<0.05)。Spearman相关性分析显示,TyG指数、RBP4水平与DR病情程度呈正相关,nesfatin-1水平与DR病情程度呈负相关(r_(s)=0.557、0.392、-0.359,均P<0.05)。Pearson相关分析显示,T2DM并发DR患者TyG指数与nesfatin-1水平呈负相关,与RBP4水平呈正相关,nesfatin-1与RBP4水平呈负相关(r=-0.486、0.538、-0.592,均P<0.05)。结论:血清TyG指数、nesfatin-1、RBP4水平与DR发病风险及病情程度有关,可作为DR早期预测的标志物,且联合预测效能更好。
基金the National Natural Science Foundation of China(Nos.81570603,81270824 and 81670632)the New-100 Talent Plan of Shanghai Jiao Tong University School of Medicine,the Shanghai Talents Development Fund(No.201350)the Shanghai Pujiang Talent Projects(No.15PJ1406700)
文摘It has been reported that retinol-binding protein 4(RBP4) is associated to adiposity,insulin resistance,and type 2 diabetes.Meanwhile,circulating RBP4 levels are also affected by renal function.The aim of the present study is to investigate whether serum levels of RBP4 are primarily associated with different stages of chronic kidney disease(CKD) or type 2 diabetes,if there is more potential relevance between RBP4 and renal replacement therapy.The serum levels of RBP4 were assessed by commercial competitive enzyme-linked immunosorbent assay(ELISA)kit in 212 patients with the CKD stages 1—5 and in 24 healthy controls,while its correlation with clinical and metabolic parameters was analyzed.The serum level of RBP4 had a strong correlation with estimated glomerular filtration rate(e GFR)(P < 0.001).Stratified by e GFR and treatment,no more differences in RBP4 serum concentration were detected between type 2 diabetic and non-diabetic subjects [CKD stages 1—5,non-dialysis(ND),hemodialysis(HD) and peritoneal dialysis(PD);P > 0.05 for all].The elevation of RBP4 become higher in HD than in PD and ND in CKD5 patients(P = 0.008 and P = 0.04,respectively),while there was no significant difference between PD and ND groups.Multivariate linear regression analysis demonstrated three independent predictors of e GFR(β =-0.676,P < 0.001),C-reactive protein(CRP)(β =-0.573,P < 0.001) and creatine(β = 0.509,P = 0.024) in the study population.The study results demonstrated that the serum level of RBP4 was negatively related to the e GFR,whether diabetes mellitus(DM) affected the blood concentration of RBP4 or not.And the serum level of RBP4 exhibited significant difference in different renal replacement therapies.
