The complete mitochondrial genomes of Asio flammeus and Asio otus were se- quenced and found to span 18858 bp and 18493 bp, respectively. It is surprising to find the for- mer to be the largest among all avian mitocho...The complete mitochondrial genomes of Asio flammeus and Asio otus were se- quenced and found to span 18858 bp and 18493 bp, respectively. It is surprising to find the for- mer to be the largest among all avian mitochondrial genomes sequenced so far. The two ge- nomes have very similar gene order with that of Gallus gallus, neither contains the pseudo con- trol region, but both have a single extra base, namely Cytidine, at position 174 in ND3 gene. The control regions of Asio flammeus and Asio otus’ mitochondrial genomes span 3288 bp and 2926 bp respectively, which are the longest among vertebrates except for Myxine glutinosa and con- tribute to the large size of two genomes. The 3′end of the control region of Asio flammeus and Asio otus contains many tandemly repeated sequences, which are highly similar to a putative control element, i.e. Mt5, and may form stable stem-loop secondary structures. Such repeated sequences probably play an important role in regulating transcription and replication of mito- chondrial genome. Our results may provide important clues for uncovering the origin and evolu- tion mechanisms of mitochondrion genome.展开更多
采用Illumina HiSeq X Ten平台测序和生物信息法对唐古特扁桃叶绿体基因组序列特征进行解析。结果表明:唐古特扁桃叶绿体基因组为四分体式结构,完整叶绿体基因组序列全长158166 bp,G+C含量36.8%,注释131个基因,即86个蛋白编码基因、8个r...采用Illumina HiSeq X Ten平台测序和生物信息法对唐古特扁桃叶绿体基因组序列特征进行解析。结果表明:唐古特扁桃叶绿体基因组为四分体式结构,完整叶绿体基因组序列全长158166 bp,G+C含量36.8%,注释131个基因,即86个蛋白编码基因、8个rRNA基因和37个tRNA基因;其叶绿体基因组共有52711个编码密码子,有33种类型是偏好密码子;唐古特扁桃叶绿体基因组中SSR位点有33个,串联重复有13个;SSR中未检测到三核苷酸重复;SSR分布不平衡,大多位于基因间区IGS和LSC区域;基于叶绿体全基因组序列构建系统发育建树,发现唐古特扁桃与同亚属的榆叶梅、野樱桃以及李属毛樱桃亲缘关系较近。展开更多
According to the distribution characteristics of short and repeated sequence (SRS), a steganaiytic method based on the correlation of image bit planes is proposed. Firstly, we provide the conception of SRS distance ...According to the distribution characteristics of short and repeated sequence (SRS), a steganaiytic method based on the correlation of image bit planes is proposed. Firstly, we provide the conception of SRS distance statistics and deduce its statistical distribution. Because the SRS distance statistics can effectively reflect the correlation of the sequence, SRS has statistical features when the image bit plane sequence equals the image width. Using this characteristic, the steganalytic method is fulfilled by the distinct test of Poisson distribution. Experimental results show a good performance for detecting LSB matching steganographic method in still images. By the way, the proposed method is not designed for specific steganographic algorithms and has good generality.展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant No.30170505).
文摘The complete mitochondrial genomes of Asio flammeus and Asio otus were se- quenced and found to span 18858 bp and 18493 bp, respectively. It is surprising to find the for- mer to be the largest among all avian mitochondrial genomes sequenced so far. The two ge- nomes have very similar gene order with that of Gallus gallus, neither contains the pseudo con- trol region, but both have a single extra base, namely Cytidine, at position 174 in ND3 gene. The control regions of Asio flammeus and Asio otus’ mitochondrial genomes span 3288 bp and 2926 bp respectively, which are the longest among vertebrates except for Myxine glutinosa and con- tribute to the large size of two genomes. The 3′end of the control region of Asio flammeus and Asio otus contains many tandemly repeated sequences, which are highly similar to a putative control element, i.e. Mt5, and may form stable stem-loop secondary structures. Such repeated sequences probably play an important role in regulating transcription and replication of mito- chondrial genome. Our results may provide important clues for uncovering the origin and evolu- tion mechanisms of mitochondrion genome.
文摘采用Illumina HiSeq X Ten平台测序和生物信息法对唐古特扁桃叶绿体基因组序列特征进行解析。结果表明:唐古特扁桃叶绿体基因组为四分体式结构,完整叶绿体基因组序列全长158166 bp,G+C含量36.8%,注释131个基因,即86个蛋白编码基因、8个rRNA基因和37个tRNA基因;其叶绿体基因组共有52711个编码密码子,有33种类型是偏好密码子;唐古特扁桃叶绿体基因组中SSR位点有33个,串联重复有13个;SSR中未检测到三核苷酸重复;SSR分布不平衡,大多位于基因间区IGS和LSC区域;基于叶绿体全基因组序列构建系统发育建树,发现唐古特扁桃与同亚属的榆叶梅、野樱桃以及李属毛樱桃亲缘关系较近。
基金the National Natural Science Foundation of China (Grant No.60473022)
文摘According to the distribution characteristics of short and repeated sequence (SRS), a steganaiytic method based on the correlation of image bit planes is proposed. Firstly, we provide the conception of SRS distance statistics and deduce its statistical distribution. Because the SRS distance statistics can effectively reflect the correlation of the sequence, SRS has statistical features when the image bit plane sequence equals the image width. Using this characteristic, the steganalytic method is fulfilled by the distinct test of Poisson distribution. Experimental results show a good performance for detecting LSB matching steganographic method in still images. By the way, the proposed method is not designed for specific steganographic algorithms and has good generality.