The Type-B authentic response regulator(ARR-Bs)gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes.However,study of ARR-Bs gene family in soy...The Type-B authentic response regulator(ARR-Bs)gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes.However,study of ARR-Bs gene family in soybean is limited.Genome-wide analysis and expression profiling of the ARR-Bs gene family were performed in the soybean genome.31 ARR-Bs genes(namely GmARR-B1-31)were identified,containing conserved catalytic domains with protein lengths and molecular weights ranging from 246 to 699 amino acids(aa)and 28.30 to 76.86 kDa,respectively.Phylogenetic analysis grouped ARR-Bs genes into three clusters—Cluster I,Cluster II,and Cluster III—which included 15,12,and 4 genes,respectively,and were asymmetrically distributed on 17 chromosomes.Tissue specific expression analysis of GmARR-Bs family revealed a high transcription level in flowers,roots and seeds.The subcellular localization of GmARR5,GmARR14 were observed in the nucleus,and the promoter region of them included low-temperature responsive element(LTR),the C-repeat/dehydration responsive element(DRE),MBS,ABRE,MYB,MEJA and TCA-elements,which possibly participate in abiotic stress and hormones responses.qRTPCR analysis showed that the expression of GmARR-B genes were affected by different abiotic stresses,especially cold stress and salt stress,and GmARR-B5 and GmARR-B14 were significantly induced by cold stress.This suggested that ARR-Bs genes were involved in multiple abiotic stress response pathways and acted as a positive factor under cold stress.展开更多
基金This work was financially supported by the National Modern Agricultural Industrial Technology System(CARS-04-01A).
文摘The Type-B authentic response regulator(ARR-Bs)gene family is one of the important plant-specific transcription factor families involved in variety of physiological processes.However,study of ARR-Bs gene family in soybean is limited.Genome-wide analysis and expression profiling of the ARR-Bs gene family were performed in the soybean genome.31 ARR-Bs genes(namely GmARR-B1-31)were identified,containing conserved catalytic domains with protein lengths and molecular weights ranging from 246 to 699 amino acids(aa)and 28.30 to 76.86 kDa,respectively.Phylogenetic analysis grouped ARR-Bs genes into three clusters—Cluster I,Cluster II,and Cluster III—which included 15,12,and 4 genes,respectively,and were asymmetrically distributed on 17 chromosomes.Tissue specific expression analysis of GmARR-Bs family revealed a high transcription level in flowers,roots and seeds.The subcellular localization of GmARR5,GmARR14 were observed in the nucleus,and the promoter region of them included low-temperature responsive element(LTR),the C-repeat/dehydration responsive element(DRE),MBS,ABRE,MYB,MEJA and TCA-elements,which possibly participate in abiotic stress and hormones responses.qRTPCR analysis showed that the expression of GmARR-B genes were affected by different abiotic stresses,especially cold stress and salt stress,and GmARR-B5 and GmARR-B14 were significantly induced by cold stress.This suggested that ARR-Bs genes were involved in multiple abiotic stress response pathways and acted as a positive factor under cold stress.
