Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques ...Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques （COSY, HMQC, HMBC, NOESY） and HR-ESIMS.展开更多
A new compound, 4-hydroxy-a-tetralone-4-O-b-D-[6-O-(3,4,5-trihydroxybenzoyl) glucopyranoside (1), together with a known compound, 4-hydroxy-a-tetralone (2), has been isolated from the roots of Juglans regia. 2 showed...A new compound, 4-hydroxy-a-tetralone-4-O-b-D-[6-O-(3,4,5-trihydroxybenzoyl) glucopyranoside (1), together with a known compound, 4-hydroxy-a-tetralone (2), has been isolated from the roots of Juglans regia. 2 showed moderate bioactivity against protein tyrosine phosphatase 1B (PTP1B).展开更多
基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,T...基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)预测核桃活性成分的作用靶点,运用OMIM、GeneCards和DrugBank数据库检索HUA疾病靶点。使用Cytoscape 3.7.2软件进行作用靶点-活性成分-疾病网络绘制,利用Metascape平台进行富集分析。使用AutoDockTools 1.5.6软件进行分子对接,对活性成分及重要靶点的结合度进行验证。结果表明,共筛选出核桃活性成分5种,与HUA有交集靶点16个;富集程度较高的信号通路包括癌症信号通路、化学致癌-受体激活信号通路、糖尿病并发症中的晚期糖基化产物-晚期糖基化终末产物受体信号通路和卡波西肉瘤相关疱疹病毒信号通路;通过分子对接验证试验可知,核桃中主要活性成分与重要靶点结合紧密。展开更多
Abstract Antioxidant supplements from plants are vital to count the oxidative damage in cells. We assessed the antioxidants and antibacterial activity of green hull of Juglans regia in this study. According to our res...Abstract Antioxidant supplements from plants are vital to count the oxidative damage in cells. We assessed the antioxidants and antibacterial activity of green hull of Juglans regia in this study. According to our results the maximum antibacterial activity was observed in ethanolic extract when compared to other extract. So, the ethanolic extract was studied for antioxidant activity which exhibited high antiradical activity against DPPH, hydroxyl, and nitric oxide radicals. In conclusion, green hull of J. regia showed strong reducing power activity and total antioxidant capacity. The results justify the therapeutic application of plant in the indigenous system of medicine.展开更多
LIM(LIN11,Isl1和MEC3)可参与细胞骨架构建、代谢调控以及生物和非生物胁迫等多种生命过程,是真核生物谱系中普遍存在的重要转录因子之一。为深入了解核桃LIM基因家族信息,本研究从核桃全基因组数据库中最终筛选确定了15个LIM基因,利用...LIM(LIN11,Isl1和MEC3)可参与细胞骨架构建、代谢调控以及生物和非生物胁迫等多种生命过程,是真核生物谱系中普遍存在的重要转录因子之一。为深入了解核桃LIM基因家族信息,本研究从核桃全基因组数据库中最终筛选确定了15个LIM基因,利用生物信息学方法对核桃LIM家族进行系统分析。结果表明,15个核桃LIM基因,其氨基酸序列长度介于189~577 aa,理论等电点介于5.56~9.08,分子量介于20.80~65.85 k D,均为疏水性蛋白,主要位于细胞核和叶绿体中,根据进化分析将其分为5个亚族。启动子区顺式作用元件分析表明,该基因家族成员受脱落酸、乙烯、茉莉酸甲酯和水杨酸4种激素调节,并能响应低温逆境。本研究基于核桃全基因组分析,鉴定出15个LIM基因家族成员,系统分析了理化性质、基因结构、染色体定位及顺式作用元件,为进一步探索核桃LIM基因家族在核桃树体响应逆境胁迫的生物学功能提供了参考依据。展开更多
The common walnut(Juglans regia)is one of the most economically important nut trees cultivated worldwide.Despite its importance,no comprehensive evaluation of walnut tree population genetics has been undertaken across...The common walnut(Juglans regia)is one of the most economically important nut trees cultivated worldwide.Despite its importance,no comprehensive evaluation of walnut tree population genetics has been undertaken across the range where it originated,Central Asia.In this study,we investigated the genetic diversity and population structure of 1082 individuals from 46 populations across Central Asia.We found moderate genetic diversity of J.regia across Central Asia,with 46 populations clustered into three groups with a weak relationship between genetic and geographic distance.Our findings reveal that the western Himalaya might be the core region of common walnut genetic diversity in Central Asia and that,except for two populations in Gongliu Wild Walnut Valley,humans might have introduced walnut populations to Xinjiang,China.The observed distribution of the genetic landscape has probably been affected by historical climate fluctuation,breeding system,and prolonged anthropogenic activity.We propose the conservation of the core genetic diversity resources in the western Himalaya and pay special attention to populations from Gongliu in Xinjiang.These findings enhance our understanding of the genetic variation throughout the distribution range of J.regia in Central Asia,which will provide a key prerequisite for evidence-based conservation and management.展开更多
文摘Two new cyclic diarylheptanoids juglanin A （1） and juglanin B （2） were isolated from the pericarps of Juglans regia L. Their structures were elucidated by various spectroscopic methods including 2D NMR techniques （COSY, HMQC, HMBC, NOESY） and HR-ESIMS.
文摘A new compound, 4-hydroxy-a-tetralone-4-O-b-D-[6-O-(3,4,5-trihydroxybenzoyl) glucopyranoside (1), together with a known compound, 4-hydroxy-a-tetralone (2), has been isolated from the roots of Juglans regia. 2 showed moderate bioactivity against protein tyrosine phosphatase 1B (PTP1B).
文摘基于网络药理学方法及分子对接技术分析核桃(Juglans regia L.)治疗高尿酸血症(Hyperuricemia,HUA)的作用机制,利用中药系统药理数据库和分析平台(Traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)预测核桃活性成分的作用靶点,运用OMIM、GeneCards和DrugBank数据库检索HUA疾病靶点。使用Cytoscape 3.7.2软件进行作用靶点-活性成分-疾病网络绘制,利用Metascape平台进行富集分析。使用AutoDockTools 1.5.6软件进行分子对接,对活性成分及重要靶点的结合度进行验证。结果表明,共筛选出核桃活性成分5种,与HUA有交集靶点16个;富集程度较高的信号通路包括癌症信号通路、化学致癌-受体激活信号通路、糖尿病并发症中的晚期糖基化产物-晚期糖基化终末产物受体信号通路和卡波西肉瘤相关疱疹病毒信号通路;通过分子对接验证试验可知,核桃中主要活性成分与重要靶点结合紧密。
文摘Abstract Antioxidant supplements from plants are vital to count the oxidative damage in cells. We assessed the antioxidants and antibacterial activity of green hull of Juglans regia in this study. According to our results the maximum antibacterial activity was observed in ethanolic extract when compared to other extract. So, the ethanolic extract was studied for antioxidant activity which exhibited high antiradical activity against DPPH, hydroxyl, and nitric oxide radicals. In conclusion, green hull of J. regia showed strong reducing power activity and total antioxidant capacity. The results justify the therapeutic application of plant in the indigenous system of medicine.
文摘LIM(LIN11,Isl1和MEC3)可参与细胞骨架构建、代谢调控以及生物和非生物胁迫等多种生命过程,是真核生物谱系中普遍存在的重要转录因子之一。为深入了解核桃LIM基因家族信息,本研究从核桃全基因组数据库中最终筛选确定了15个LIM基因,利用生物信息学方法对核桃LIM家族进行系统分析。结果表明,15个核桃LIM基因,其氨基酸序列长度介于189~577 aa,理论等电点介于5.56~9.08,分子量介于20.80~65.85 k D,均为疏水性蛋白,主要位于细胞核和叶绿体中,根据进化分析将其分为5个亚族。启动子区顺式作用元件分析表明,该基因家族成员受脱落酸、乙烯、茉莉酸甲酯和水杨酸4种激素调节,并能响应低温逆境。本研究基于核桃全基因组分析,鉴定出15个LIM基因家族成员,系统分析了理化性质、基因结构、染色体定位及顺式作用元件,为进一步探索核桃LIM基因家族在核桃树体响应逆境胁迫的生物学功能提供了参考依据。
基金supported by grants from the National Natural Science Foundation of China(32170398,42211540718,32260149,41971071)the Top-notch Young Talents Project of Yunnan Provincial“Ten Thousand Talents Program”(YNWR-QNBJ-2018-146)+5 种基金CAS“Light ofWest China”Program,and Natural Science Foundation of Yunnan(202201AT070222)the Fund of Yunnan Key Laboratory of Crop Wild Relatives Omics(CWR-2024-04)the Jiangxi Provincial Natural Science Foundation(20224BAB215012)the Science and Technology Research Project of Jiangxi Provincial Department of Education(GJJ2202401)Key Research Program of Frontier Sciences,CAS(ZDBSLY-7001)Yunnan Fundamental Research Projects(202201BC070001).
文摘The common walnut(Juglans regia)is one of the most economically important nut trees cultivated worldwide.Despite its importance,no comprehensive evaluation of walnut tree population genetics has been undertaken across the range where it originated,Central Asia.In this study,we investigated the genetic diversity and population structure of 1082 individuals from 46 populations across Central Asia.We found moderate genetic diversity of J.regia across Central Asia,with 46 populations clustered into three groups with a weak relationship between genetic and geographic distance.Our findings reveal that the western Himalaya might be the core region of common walnut genetic diversity in Central Asia and that,except for two populations in Gongliu Wild Walnut Valley,humans might have introduced walnut populations to Xinjiang,China.The observed distribution of the genetic landscape has probably been affected by historical climate fluctuation,breeding system,and prolonged anthropogenic activity.We propose the conservation of the core genetic diversity resources in the western Himalaya and pay special attention to populations from Gongliu in Xinjiang.These findings enhance our understanding of the genetic variation throughout the distribution range of J.regia in Central Asia,which will provide a key prerequisite for evidence-based conservation and management.
