This study attempted to culture red sea bream lymphocytes using artificial medium supplemented with mitogens and a cytokine. Growth of the lymphocytes was compared between cultures with mitogens phytohemagglutinin (PH...This study attempted to culture red sea bream lymphocytes using artificial medium supplemented with mitogens and a cytokine. Growth of the lymphocytes was compared between cultures with mitogens phytohemagglutinin (PHA), lipopolysaccharide (LPS), concanavalin A(Con A) and a cytokine mouse interleukin 2(IL 2) in a basal medium at various combinations. Addition of PHA or LPS enhanced growth of the lymphocytes, Con A did not enhance growth so much. Large colonies were formed when PA was added. Con A induced scattered single cell proliferation of the lymphocytes. IL 2 supported the actions of PHA and LPS. Combined addition of PHA and IL 2 maintained proliferation for 30 d.展开更多
文摘This study attempted to culture red sea bream lymphocytes using artificial medium supplemented with mitogens and a cytokine. Growth of the lymphocytes was compared between cultures with mitogens phytohemagglutinin (PHA), lipopolysaccharide (LPS), concanavalin A(Con A) and a cytokine mouse interleukin 2(IL 2) in a basal medium at various combinations. Addition of PHA or LPS enhanced growth of the lymphocytes, Con A did not enhance growth so much. Large colonies were formed when PA was added. Con A induced scattered single cell proliferation of the lymphocytes. IL 2 supported the actions of PHA and LPS. Combined addition of PHA and IL 2 maintained proliferation for 30 d.
