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木质素生物合成及其基因调控研究综述 被引量:23
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作者 陈永忠 谭晓风 David Clapham 《江西农业大学学报》 CAS CSCD 2003年第4期613-617,共5页
木质素是木材中仅次于纤维素的主要成分 ,占木材干重 1 5 %~ 3 6% ,对造纸业有很重要的影响。通过探索其生物合成途径 ,针对合成过程中的不同酶系 :PAL、C4H、4CL、CCR、CAD、COMT等 ,采用基因工程的反义技术构建调控基因进行转化 ,可... 木质素是木材中仅次于纤维素的主要成分 ,占木材干重 1 5 %~ 3 6% ,对造纸业有很重要的影响。通过探索其生物合成途径 ,针对合成过程中的不同酶系 :PAL、C4H、4CL、CCR、CAD、COMT等 ,采用基因工程的反义技术构建调控基因进行转化 ,可以达到降低木质素含量、改变其化学结构和组织的目的。综述了用于木质素基因工程、合成酶基因的克隆和表达研究现状 ,并指出了功能基因组和基因芯片技术在木质素合成途径研究中的应用前景。 展开更多
关键词 木质素 生物合成 基因调控 反义技术 隐性突变体
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一个来源于隐性高秆水稻“02428h”的矮秆迟熟突变体“02428ha” 被引量:10
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作者 王才林 赵凌 周寿芳 《江苏农业学报》 CSCD 2003年第3期129-132,共4页
 在隐性高秆水稻"02428h"中发现1个矮秆迟熟突变体"02428ha"。该突变体与02428h相比,抽穗期延迟32d左右,表现很强的感光性;株高降低60 0cm左右,主要是倒1、倒2节间长度变短,分别缩短34 0cm和19 0cm;倒1和倒2节间...  在隐性高秆水稻"02428h"中发现1个矮秆迟熟突变体"02428ha"。该突变体与02428h相比,抽穗期延迟32d左右,表现很强的感光性;株高降低60 0cm左右,主要是倒1、倒2节间长度变短,分别缩短34 0cm和19 0cm;倒1和倒2节间的株高构成系数(各节间长度占株高的百分比)分别降低8 6和3 1个百分点;穗长缩短10 0cm,每穗一次枝梗数减少2 4个,每穗总粒数和每穗实粒数分别减少125 0粒和108 0粒,但结实率无明显差异。与02428h一样,02428ha也具有广亲和特性。 展开更多
关键词 隐性高秆水稻 “02428h” 矮秆迟熟突变体 “02428ha” 抽穗期 感光性 株高 构成系数
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多核丝状真菌的选育及遗传稳定性 被引量:10
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作者 陆茂林 单志萍 孟妤 《生物技术》 CAS CSCD 2002年第2期16-18,共3页
细胞中多核体的存在 ,对其隐性突变体的分离及优良形状的稳定遗传产生了相当大的障碍。通过采用大剂量的NTG诱变多核单细胞丝状真菌三孢布拉霉JMβ817的孢子 ,再经紫外线复合照射该孢子制备成的原生质体 ,使其高产菌株———超黄突变体... 细胞中多核体的存在 ,对其隐性突变体的分离及优良形状的稳定遗传产生了相当大的障碍。通过采用大剂量的NTG诱变多核单细胞丝状真菌三孢布拉霉JMβ817的孢子 ,再经紫外线复合照射该孢子制备成的原生质体 ,使其高产菌株———超黄突变体的隐性基因CarS和CarD得以表达 ,从中分离到一株CarS隐性突变株JMβ2 87,β -胡萝卜的产量达到 2 36g/l,比出发菌株提高了 30 %。并通过孢子的亚培养进行定向选育 ,使高产性状得以稳定遗传 ,为促进工业化发酵生产天然 β -胡萝卜提供了必要的手段。 展开更多
关键词 选育 丝状真菌 多核体 隐性突变 遗传稳定性
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Pigmentation Restored in Mutant Laboratory Strain of the Lady Beetle Coleomegilla maculata through Dietary Supplementation 被引量:1
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作者 Margaret Louise Allen 《Advances in Entomology》 2016年第3期133-140,共8页
A laboratory colony of Coleomegilla maculata (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colored parental strain... A laboratory colony of Coleomegilla maculata (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colored parental strain to the diet. While the wings and other colored portions of the cuticle re-gained the red color, the eyes of the pigmentation deficient insects were not changed from the pale mutant form. Plant derived carotenes lycopene and beta-carotene did not restore the mutant beetles to a visibly distinguishable red color. An additional pigmentation deficient mutant strain, gold, partially recovered red cuticle color when provided with diet containing pigmented insect particles. This work represents the first rescue of a color phenotype in a lady beetle. 展开更多
关键词 Lady Beetle recessive Phenotype Carotenoid Pigments Forward Genetics mutant Rescue Dietary Supplement
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具有隐性高秆基因的矮秆迟熟突变体“02428ha”的遗传分析 被引量:2
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作者 王才林 赵凌 周寿芳 《江苏农业学报》 CSCD 2003年第4期193-197,共5页
 "02428ha"是从隐性高秆水稻"02428h"中发现的1个矮秆迟熟突变体。其抽穗期比02428h迟32d左右,株高降低60cm左右。遗传分析表明,02428ha矮秆性状的遗传涉及2对基因,而迟熟感光性则受1对显性基因控制。
关键词 隐性高秆基因 矮秆迟熟突变体 “02428ha” 遗传分析 水稻 迟熟感光性
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Genetics and Characteristics of a Pigmentation Defective Laboratory Strain of the Lady Beetle, Coleomegilla maculata
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作者 Margaret Louise Allen Joseph Grey Ballenger 《Advances in Entomology》 2014年第4期161-166,共6页
Beetles in the family Coccinellidae, commonly known as ladybugs, lady beetles, or ladybirds, are easily identifiable and popular beneficial insects. Current research aims to support conservation efforts of beneficial ... Beetles in the family Coccinellidae, commonly known as ladybugs, lady beetles, or ladybirds, are easily identifiable and popular beneficial insects. Current research aims to support conservation efforts of beneficial insects in agroecosystems by exploring genetic processes related to nutrition. As a part of this research, colonies of Coleomegilla maculata have been maintained in culture and inbred over many generations since 2009. One result of this inbreeding has been the discovery of novel morphological phenotypes unique to laboratory strains or present in wild populations at such low levels that they have not yet been described. One such phenotype is described here. The strain described here, ye (yellow elytra and eyes) was characterized with classical Mendelian breeding and digital image analysis. This phenotype differs from wild populations by possessing yellow pigment in the elytra and pale grey to white eyes. In contrast, wild populations of C. maculata possess pink or red pigmented elytra with black spots, and black eyes. C. maculata is not known to exhibit polymorphism in the field. Inheritance is autosomal and recessive. This species was not previously known to exhibit the dramatic variation of color described here. The strain is stable in the homozygous recessive form, and retains laboratory rearing characteristics similar to the wild type laboratory strain. 展开更多
关键词 COCCINELLIDAE Yellow Autosomal recessive ELYTRA Eye Color INBREEDING Heritable TRAIT PIGMENTATION mutant Phenotype Cuticle
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Pigmentation Restored in Mutant Laboratory Strain of the Lady Beetle <i>Coleomegilla maculata</i>through Dietary Supplementation
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作者 Margaret Louise Allen 《Advances in Entomology》 2016年第3期133-140,共8页
A laboratory colony of <i>Coleomegilla maculata</i> (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colo... A laboratory colony of <i>Coleomegilla maculata</i> (DeGeer), ye, selected for a pigmentation deficiency, was restored to near wild type cuticle coloration by adding crushed heads and wings of the red colored parental strain to the diet. While the wings and other colored portions of the cuticle re-gained the red color, the eyes of the pigmentation deficient insects were not changed from the pale mutant form. Plant derived carotenes lycopene and beta-carotene did not restore the mutant beetles to a visibly distinguishable red color. An additional pigmentation deficient mutant strain, gold, partially recovered red cuticle color when provided with diet containing pigmented insect particles. This work represents the first rescue of a color phenotype in a lady beetle. 展开更多
关键词 Lady Beetle recessive Phenotype Carotenoid Pigments Forward Genetics mutant Rescue Dietary Supplement
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Nutritional Quality of the Maize Combinations with Mutant Gene opaque-2 (o2)
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作者 宋丽雅 陈亮 +4 位作者 何聪芬 赵刚 白鹏飞 陈岩 常驰 《Agricultural Science & Technology》 CAS 2012年第6期1179-1183,1202,共6页
[Objective] This study aimed to investigate the action of mutant gene o2 and its effect on nutritional quality of different maize combinations. [Method] A total of 33 normal maize combinations from 18 inbred lines wer... [Objective] This study aimed to investigate the action of mutant gene o2 and its effect on nutritional quality of different maize combinations. [Method] A total of 33 normal maize combinations from 18 inbred lines were compared with 33 combinations including gene o2 from the corresponding o2 near-isogenic lines (o2-NILs), to study the effect of o2 gene introduction on maize grain quality. [Result] The contents of lysine, protein and oil in o2-NILs were greatly more than that of normal maize combinations. Except for lysine, contents of other 14 amino acids changed when o2 gene was introduced. Contents of aspartic acid, threonine, glycine, isoleucine, histidine, arginine and proline were improved; while contents of serine, glutamic acid, alanine, valine, leucine, tyrosine and phenylalanine were decreased. Correlation analysis showed that contents of aspartic acid, arginine and threonine had the highest correlation with lysine content. Protein and oil contents had higher correlation with lysine content (0.48 and 0.38). Analysis of 33 o2-NILs revealed that the o2 combinations CAL58×Ji477and CA156×196 showed high comprehensive quality and high yield with greater development potential. [Conclusion] This study will provide theoretical and material basis for improving the quality of temperate maize germplasm by introducing o2 gene. 展开更多
关键词 Corn Opaque-2 homozygous recessive mutant gene (o2) LYSINE Amino acids Protein
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Associations of the decoy receptor and osteoprotegerin gene polymorphisms with ulcerative colitis in Chinese patients
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作者 郑香云 《China Medical Abstracts(Internal Medicine)》 2014年第3期172-173,共2页
Objective To investigate the correlation between decoy receptor(DcR)1,DcR2 and osteoprotegerin(OPG)gene polymorphisms with the susceptibility to ulcerative colitis(UC)in Chinese population.Methods A total of352 patien... Objective To investigate the correlation between decoy receptor(DcR)1,DcR2 and osteoprotegerin(OPG)gene polymorphisms with the susceptibility to ulcerative colitis(UC)in Chinese population.Methods A total of352 patients with UC as well as 463 sex-and agematched healthy controls were recruited in the study.The genetic polymorphisms of DcR1(rs12549481),DcR2(rs1133782)and OPG(rs3102735)were deter- 展开更多
关键词 DECOY susceptibility deter ALLELE sequencing recessive mutant autosomal CONFORM mined
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一个新的水稻隐性高秆突变体的遗传分析和基因定位 被引量:9
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作者 马玉银 李磊 +5 位作者 李育红 左示敏 殷跃军 张亚芳 陈宗祥 潘学彪 《中国农业科学》 CAS CSCD 北大核心 2008年第12期3967-3973,共7页
【目的】寻找新的水稻隐性高秆基因,为解决水稻不育系的包颈现象及增加恢复系的株高提供重要的遗传资源。【方法】在田间试验时发现一株高秆突变体,经过连续3代的自交和选择后,获得稳定遗传的高秆突变体。突变体的株高比野生型中花11平... 【目的】寻找新的水稻隐性高秆基因,为解决水稻不育系的包颈现象及增加恢复系的株高提供重要的遗传资源。【方法】在田间试验时发现一株高秆突变体,经过连续3代的自交和选择后,获得稳定遗传的高秆突变体。突变体的株高比野生型中花11平均增加72.3%。将带有高秆基因的杂合体自交,分析F2代株高的遗传行为,结果表明高秆性状由一对隐性基因控制。分别配制突变体、野生型与培矮64之间的杂交种及其自交F2分离群体,在突变体配制的分离群体中鉴定出72株极端高秆突变体,株高明显高于对照群体中的最高植株高度。【结果】利用均匀分布于水稻12条染色体上的147对多态性分子标记,分析这些标记位点在极端高秆突变体群体中的分离特征,结果证明高秆基因位于水稻第3染色体。进一步发展了4个InDel标记,确定高秆基因位于RM168与M127.1-3-3标记之间,物理距离为713 kb。【结论】该基因是一个新的隐性高秆基因,暂命名为lc3。 展开更多
关键词 水稻 隐性高秆突变体 高秆基因 基因定位 微卫星标记 INDEL标记
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利用遗传突变基因改良特用玉米 Ⅴ.甜玉米胚乳突变基因的效应与利用 被引量:6
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作者 秦泰辰 邓德祥 +1 位作者 蒋守华 卞云龙 《玉米科学》 CAS CSCD 2004年第3期9-12,共4页
简要说明甜玉米突变基因由来以及研究近况。甜玉米在碳水化合物中形成的隐性突变基因占有相当大的比例,其种源丰富,已育成多个品种。在育种技术方面,着重介绍新方法——转座子育种技术。
关键词 甜玉米 水溶性多糖 隐性突变基因 se基因
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A 2-bp insertion(c.6768insCC) in MC1R causes recessive white coat color in Bama miniature pigs 被引量:5
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作者 Qitao Jia Chunwei Cao +11 位作者 Hai Tang Ying Zhang Qiantao Zheng Xiao Wang Rui Zhang Xianlong Wang Ailing Luo Hong Wei Anming Meng Qi Zhou Hongmei Wang Jianguo Zhao 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2017年第4期215-217,共3页
Coat color is an important characteristic of various breeds of domestic animal species.Variation in farm animal coat color is of considerable interest for concealment,communication and protection against solar radiat... Coat color is an important characteristic of various breeds of domestic animal species.Variation in farm animal coat color is of considerable interest for concealment,communication and protection against solar radiation(Slominski et al.,2004).It also plays an important role in the regulation of physiological processes(Miyagi and Terai,2013). 展开更多
关键词 miniature recessive c.67_68insCC insertion physiological mutant originally phenotype spots linkage
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Opaque-2突变基因(o2)对玉米组合品质的影响 被引量:2
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作者 宋丽雅 陈亮 +4 位作者 何聪芬 赵刚 白鹏飞 陈岩 常驰 《安徽农业科学》 CAS 2012年第18期9607-9609,9665,共4页
[目的]探讨o2突变基因的作用方式,提高玉米品质育种研究水平。[方法]通过对18个普通玉米自交系组配的33个普通玉米组合及相应的导入o2近等基因系(o2-NILs)组配的33个含o2玉米组合品质的比较,分析了导入o2后对玉米组合品质的影响。[结果]... [目的]探讨o2突变基因的作用方式,提高玉米品质育种研究水平。[方法]通过对18个普通玉米自交系组配的33个普通玉米组合及相应的导入o2近等基因系(o2-NILs)组配的33个含o2玉米组合品质的比较,分析了导入o2后对玉米组合品质的影响。[结果]o2-NILs组合与普通玉米组合相比,其赖氨酸、蛋白质和油份含量有大幅增加,其余14种必须氨基酸含量也发生了相应的变化,天门冬氨酸、苏氨酸、甘氨酸、异亮氨酸、组氨酸、精氨酸和脯氨酸含量提高;而丝氨酸、谷氨酸、丙氨酸、缬氨酸、亮氨酸、酪氨酸和苯丙氨酸含量降低。相关性分析结果表明,天门冬氨酸、精氨酸和苏氨酸变化与赖氨酸变化程度高度相关(>0.8),蛋白质和油份的变化与赖氨酸变化的相关性分别为0.48和0.38,表现为中度相关。33个o2-NILs组合综合品质分析结果表明,o2组合的CAL58×吉477和CA156×196综合品质优良,产量较高,可能具有较大的开发潜力。[结论]该研究结果为今后利用o2对温带玉米种质进行品质改良,并提高改良效率提供了理论和材料基础。 展开更多
关键词 玉米 Opaque-2隐性纯合突变基因(o2) 赖氨酸 蛋白质
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隐性高秆水稻02428h的eui基因在育种上的利用 被引量:1
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作者 王才林 赵凌 +1 位作者 朱镇 张亚东 《中国水稻科学》 CAS CSCD 北大核心 2006年第6期610-614,共5页
为研究隐性高秆水稻“02428h”的长穗颈基因eui在减轻不育系包颈度中的利用价值,以02428h为父本,培矮64S为母本和轮回亲本,经过连续3次回交将02428h的eui基因导入培矮64S,转育成了具有长穗颈基因的“P8hS”。与培矮64S相比,P8hS的株高增... 为研究隐性高秆水稻“02428h”的长穗颈基因eui在减轻不育系包颈度中的利用价值,以02428h为父本,培矮64S为母本和轮回亲本,经过连续3次回交将02428h的eui基因导入培矮64S,转育成了具有长穗颈基因的“P8hS”。与培矮64S相比,P8hS的株高增加35.6 cm,主要是穗颈节和倒2节间长度增加所致。eui基因的导入不仅可使培矮64S的穗包颈得到彻底改善,而且对培矮64S及其杂种的主要经济性状具有促进作用。 展开更多
关键词 水稻 隐性高秆突变体 长穗颈基因 遗传 回交转育 育种 不育系
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THE THREE FLAVOURINGS IN THE SEX-LINKED RECESSIVE LETHAL TEST OF DROSOPHILA MELANOGASTER
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《癌变.畸变.突变》 CAS CSCD 1991年第S1期245-245,共1页
Since Cinnamomum japonicum,Illicium verum and Zanthoxylumbungeanum are People’s favourites,it seems to be necessary toevaluate their safety accurately.Wild-type Oregon K strainof Drosophila melanogaster and Mutant st... Since Cinnamomum japonicum,Illicium verum and Zanthoxylumbungeanum are People’s favourites,it seems to be necessary toevaluate their safety accurately.Wild-type Oregon K strainof Drosophila melanogaster and Mutant stock of Base(Muller-5)were used in the test.From F<sub>2</sub> generation the mutagenicity hadbeen detected in three stages of germ-cell development by sex-linked recessive lethal test of Drosophila in negative control, 展开更多
关键词 recessive CINNAMOMUM ILLICIUM Drosophila LETHAL Muller mutant Oregon stock mutation
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