BACKGROUND: The prognosis of hepatocellular carcinoma (HCC) is poor and its early diagnosis is of the utmost importance. This study aimed to investigate the values of glypican-3 (GPC-3) expression in the liver and ser...BACKGROUND: The prognosis of hepatocellular carcinoma (HCC) is poor and its early diagnosis is of the utmost importance. This study aimed to investigate the values of glypican-3 (GPC-3) expression in the liver and sera and its gene transcription for diagnosis and monitoring of metastasis of HCC. METHODS: Liver GPC-3 was analyzed in HCC tissues from 36 patients by immunohistochemistry and Western blotting. GPC-3 mRNA from circulating peripheral blood mononuclear cells from 123 HCC patients or 246 patients with other diseases or 36 HCC tissues was amplified by RT-PCR, quantitative realtime PCR, and confirmed by DNA sequencing. Circulating GPC-3 level was detected by ELISA. RESULTS: The increasing expression of GPC-3 was observed from non-cancerous to cancerous tissues, with brown granule-like staining localized in tumor parts of atypical hyperplasia and HCC formation. The positive rate of GPC-3 was 80.6% in HCC, 41.7% in their paracancerous tissues, and none in distal cancerous tissues (P【0.001), with no significant difference in differentiation grade and tumor number except for size (Z=2.941, P=0.003). Serum GPC-3 was detected only in HCC (52.8%) and significant difference was found between GPC-3 and tumor size (χ2 =6.318, P=0.012) or HBV infection (χ2 =23.362, P【0.001). Circulating GPC-3 mRNA was detected in 70.7% of HCC tissues, with relation to TNM stage, periportal cancerous embolus, and extra-hepatic metastasis (P【0.001). The combination ofcirculating GPC-3, GPC-3 mRNA and alpha-fetoprotein is of complementary value for HCC diagnosis (94.3%). CONCLUSION: Both GPC-3 overexpression and GPC-3 mRNA abnormality could be used as markers for the diagnosis of HCC and monitoring its metastasis.展开更多
针对室外街道的行人检测与跟踪,提出一种改进YOLOv3与简单在线实时跟踪(simple online and real-time tracking,SORT)算法相结合的检测及跟踪方法。首先,引入距离和比例交并比(distance and proportional-IOU,DPIOU)损失,将原有的损失...针对室外街道的行人检测与跟踪,提出一种改进YOLOv3与简单在线实时跟踪(simple online and real-time tracking,SORT)算法相结合的检测及跟踪方法。首先,引入距离和比例交并比(distance and proportional-IOU,DPIOU)损失,将原有的损失函数中的均方误差(mean square error,MSE)部分进行变化,从而得到更精确的检测框;其次,将网络结构中的RestNet进行优化,改变下采样区域,增加池化层,进而减少特征信息的丢失;最后将检测结果输入SORT算法进行建模和匹配。实验结果表明,在室外街道的场景下,改进的算法与YOLOv3相比较,损失值收敛更快,平均准确率高出4.85%,跟踪准确率上升3.4%,同时,模型的速度有所提高,最快可达14.39 FPS。展开更多
Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP mo...Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP model, G\|GTP is used for the real\|3D modeling of subsurface geological bodies, and E\|GTP is used for the real\|3D modeling of subsurface engineering excavations.In the light of the discussions on the features and functions of E 2 GIS, the modeling principles of G\|GTP and E\|GTP are introduced. The two models couple together seamlessly to form an integral model for subsurface spatial objects including both geological bodies and excavations. An object\|oriented integral real\|3D data model and integral spatial topological relations are discussed.展开更多
基金supported in part by grants-in-Aid from the Projects of Jiangsu Medical Science (HK201102, H200925)the Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD)the Program of Nantong Society Undertaking and Technological Innovation (HS2011012),China
文摘BACKGROUND: The prognosis of hepatocellular carcinoma (HCC) is poor and its early diagnosis is of the utmost importance. This study aimed to investigate the values of glypican-3 (GPC-3) expression in the liver and sera and its gene transcription for diagnosis and monitoring of metastasis of HCC. METHODS: Liver GPC-3 was analyzed in HCC tissues from 36 patients by immunohistochemistry and Western blotting. GPC-3 mRNA from circulating peripheral blood mononuclear cells from 123 HCC patients or 246 patients with other diseases or 36 HCC tissues was amplified by RT-PCR, quantitative realtime PCR, and confirmed by DNA sequencing. Circulating GPC-3 level was detected by ELISA. RESULTS: The increasing expression of GPC-3 was observed from non-cancerous to cancerous tissues, with brown granule-like staining localized in tumor parts of atypical hyperplasia and HCC formation. The positive rate of GPC-3 was 80.6% in HCC, 41.7% in their paracancerous tissues, and none in distal cancerous tissues (P【0.001), with no significant difference in differentiation grade and tumor number except for size (Z=2.941, P=0.003). Serum GPC-3 was detected only in HCC (52.8%) and significant difference was found between GPC-3 and tumor size (χ2 =6.318, P=0.012) or HBV infection (χ2 =23.362, P【0.001). Circulating GPC-3 mRNA was detected in 70.7% of HCC tissues, with relation to TNM stage, periportal cancerous embolus, and extra-hepatic metastasis (P【0.001). The combination ofcirculating GPC-3, GPC-3 mRNA and alpha-fetoprotein is of complementary value for HCC diagnosis (94.3%). CONCLUSION: Both GPC-3 overexpression and GPC-3 mRNA abnormality could be used as markers for the diagnosis of HCC and monitoring its metastasis.
文摘针对室外街道的行人检测与跟踪,提出一种改进YOLOv3与简单在线实时跟踪(simple online and real-time tracking,SORT)算法相结合的检测及跟踪方法。首先,引入距离和比例交并比(distance and proportional-IOU,DPIOU)损失,将原有的损失函数中的均方误差(mean square error,MSE)部分进行变化,从而得到更精确的检测框;其次,将网络结构中的RestNet进行优化,改变下采样区域,增加池化层,进而减少特征信息的丢失;最后将检测结果输入SORT算法进行建模和匹配。实验结果表明,在室外街道的场景下,改进的算法与YOLOv3相比较,损失值收敛更快,平均准确率高出4.85%,跟踪准确率上升3.4%,同时,模型的速度有所提高,最快可达14.39 FPS。
文摘Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP model, G\|GTP is used for the real\|3D modeling of subsurface geological bodies, and E\|GTP is used for the real\|3D modeling of subsurface engineering excavations.In the light of the discussions on the features and functions of E 2 GIS, the modeling principles of G\|GTP and E\|GTP are introduced. The two models couple together seamlessly to form an integral model for subsurface spatial objects including both geological bodies and excavations. An object\|oriented integral real\|3D data model and integral spatial topological relations are discussed.
