Objective To study 4 cases of adult T cell leukemia/lymphoma (ATLL) as sociated with cutaneous lesions for clinicopathology, immunophenotype, human T cell leukemia/lymphoma virus type Ⅰ (HTLV Ⅰ) provirus DNA an...Objective To study 4 cases of adult T cell leukemia/lymphoma (ATLL) as sociated with cutaneous lesions for clinicopathology, immunophenotype, human T cell leukemia/lymphoma virus type Ⅰ (HTLV Ⅰ) provirus DNA and their ultrastru cture. At the same time, HTLV Ⅰ provirus DNA of ATLL patients were also compar ed with 18 cases of cutaneous lymphoma (CL), two cases of actinic reticuloid as well as two cases of lymphocytic infiltration.Methods Immunohistochemistry studies were carried out on the infiltrati ng cells using monoclonal antibodies against CD45 RO, CD20, CD68 on paraffin e mbedded sections by ABC method and using monoclonal antibodies against CD3, CD4 and CD8 with indirect immunofluorescence (IIF) on frozen sections. Skin biopsies were examined by electron microscope. Serum and bone marrow cells were tested f or antibodies against HTLV Ⅰ associated antigen by IIF, and HTLV Ⅰ provirus DNA was examined by PCR method.Results The research showed four patients with ATLL manifesting cutaneo u s lesions, were subsequently found with additional systemic symptoms, as extensi vely enlarged superficial lymph node, abnormal increased IL 2 receptor, flower like cells in their peripheral blood and marrow. The HTLV Ⅰ provirus DNA was positi ve in the peripheral blood, bone marrow, cutaneous lesions and lymph node biopsy specimens by using PCR amplification of specific HTLV Ⅰ fragment while 18 cas es of the CL were negative for HTLV Ⅰ. The special ultrastructure of skin lesi ons was also found in ATLL patients.Conclusions The cutaneous involvement in ATLL is a type of cutaneous T cell lymphoma (CTCL) but shows some differential immunological markers for diffe rential diagnosis. The examination of HTLV Ⅰ antibodies or HTLV Ⅰ provirus D NA is necessary for diagnosis of ATLL. The ultrastrustural characteristics in skin lesions of ATLL were of atypic al lymphocytes and mononuclear cells invading the epidermis, and the mononuclear cells are possessing the phagocytic function and 展开更多
The identification of antemortem burns and postmortem burns is essential in forensic practice.In this study,microRNA(miRNA)microarray analysis was conducted to identify differentially expressed miRNAs in the skin of a...The identification of antemortem burns and postmortem burns is essential in forensic practice.In this study,microRNA(miRNA)microarray analysis was conducted to identify differentially expressed miRNAs in the skin of an experimental burn model.Microarray analysis revealed 24 differentially expressed miRNAs in antemortem burned mice skin,with 19 miRNAs significantly upregulated and 5 downregulated.Based on the intersection predicted using three databases(Targetscan,microRNA.org,and PITA),293 potential miRNA targets were identified.These dysregulated miRNAs and their predicted targets were further analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases.Several functional categories and signaling pathways were enriched,including the“fc epsilon ri signaling pathway,”“endometrial cancer,”and“mTOR signaling pathway.”Expression patterns of 10 differentially expressed miRNAs were verified by reverse transcription‑quantitative polymerase chain reaction in mice skins.The results agreed with the results of microarray analysis.These findings suggest that differentially expressed miRNAs revealed by microarray are potential markers for forensic molecular diagnosis of antemortem burns.展开更多
The sensitized fluorescence intensity of terbium ion can be notably enhanced when the Tb 3+ fleroxacin complex is exposed to 365 nm light. By the measurements of fluorescence spectra, phosphorescence spectra, fl...The sensitized fluorescence intensity of terbium ion can be notably enhanced when the Tb 3+ fleroxacin complex is exposed to 365 nm light. By the measurements of fluorescence spectra, phosphorescence spectra, fluorescence quantum yield and fluorescence lifetime of the system, it is proved that irradiation makes the complex undergo a photochemical reaction and produces a new terbium complex which is more favorable to intramolecular energy transfer. The mechanism of the photochemical fluorescence enhancement was discussed.展开更多
Performance breakthrough of electrocatalysts highly relies on the regulation of internal structures and electronic states.In present work,for the first time,we successfully synthesized nitrogen doped FeS_(2) nanoparti...Performance breakthrough of electrocatalysts highly relies on the regulation of internal structures and electronic states.In present work,for the first time,we successfully synthesized nitrogen doped FeS_(2) nanoparticles(N-FeS_(2))as the electrocatalysts for hydrogen evolution reaction(HER).The band structure and electronic state of FeS_(2) are modulated by a nitrogen doping strategy,as confirmed by X-ray photoelectron spectroscopy(XPS),X-ray absorption spectroscopy(XAS)and density functional theory(DFT)calculations.Owing to the band structure and electronic state regulation as well as the weakening of H-S interaction,the designed N-FeS_(2) electrocatalyst exhibits superior catalytic performance with a low overpotential(~126 mV at 10 mA cm^(-2))and excellent activity stability under alkaline conditions,which is substantially improved as compared with that of the pure FeS_(2) counterpart.Our work demonstrates that the modulation of electron state and band structure of an electrocatalyst,which can provide a valuable guidance for designing excellent catalysts for hydrogen evolution reaction and beyond.展开更多
Developing highly efficient nickel or iron based hydroxide electrocatalysts is primary essential but challenging for oxygen evolution reaction(OER)at ultra-high current densities.Herein,we developed a facile method to...Developing highly efficient nickel or iron based hydroxide electrocatalysts is primary essential but challenging for oxygen evolution reaction(OER)at ultra-high current densities.Herein,we developed a facile method to prepare nitrogen and iron doped nickel(Ⅱ)hydroxide nanosheets on self-supported conductive nickel foam(denoted as Fe,N-Ni(OH)_(2)/NF)through ammonia hydrothermal and impregnation methods.Owing to the optimization of the electronic structure by nitrogen doping and the strong synergistic effect between Fe and Ni(OH)_(2),the three-dimensional(3 D)Fe,N-Ni(OH)_(2)/NF nanosheets delivered superior electrocatalytic OER performances in basic solution with low potentials of 1.57 V and1.59 V under 500 mA/cm^(2)and 1000 mA/cm^(2)respectively and robust operation for 10 h with ignored activity decay,comparing well with the potentials of previously reported NiFe based electrocatalysts as well as the benchmark commercial Ir/C/NF.In-situ Raman spectroscopy revealed that the main active species were NiOOH during the OER process.The present results are expected to provide new insights into the study of OER process towards ultra-high current densities.展开更多
Searching for high-activity,stabilily and highly casbellective electrocalalysts for acid oxygen reaction rolutioo(ORR)has always been a urgent problem in polymer ectrolyte menbrane fuel ells(PEMFC).Nonetheless,the ele...Searching for high-activity,stabilily and highly casbellective electrocalalysts for acid oxygen reaction rolutioo(ORR)has always been a urgent problem in polymer ectrolyte menbrane fuel ells(PEMFC).Nonetheless,the electrochemical poperties of various systems have their intrinsic limits and tremendous eforts have been paid oul to search for highly eficient electocatalysts by more raional control over the size,morphology,compoition,and structure.In particular,single-atom catalysts(SACs)have atrascted extensive intenest due to theirs cxcellant activity,stability,slctivity and the highest metal tiliztion In rceat yeurs,the number of papers in the field of SACs has incressed rapidly,indicating that SACs have made great progress.This review foouses on SACs electo-echemical applications in the acid ORR and introduces immovative syntheses,fiuel cell performance and long-time durabilily.展开更多
Visible-light-driven photochemical Cadogan-type cyclization has been discovered.The organic D-A type photosensitizer 4 CzIPN found to be an efficient mediator to transfer energy from photons to the transient intermedi...Visible-light-driven photochemical Cadogan-type cyclization has been discovered.The organic D-A type photosensitizer 4 CzIPN found to be an efficient mediator to transfer energy from photons to the transient intermediate that breaks the barriers of deoxygenation in Cadogan reaction and enables a mild metalfree access to carbazoles and related heterocycles.DFT calculation results indicate mildly endergonic formation of the intermediate complex of nitrobiarenes and PPh3,which corresponds with experimental findings regarding reaction temperature.The robust synthetic capacity of the photoredox Cadogan reaction systems has been demonstrated by the viable productivity of a broad range of carbazoles and related N-heterocycles with good tolerance of various functionalities.展开更多
文摘Objective To study 4 cases of adult T cell leukemia/lymphoma (ATLL) as sociated with cutaneous lesions for clinicopathology, immunophenotype, human T cell leukemia/lymphoma virus type Ⅰ (HTLV Ⅰ) provirus DNA and their ultrastru cture. At the same time, HTLV Ⅰ provirus DNA of ATLL patients were also compar ed with 18 cases of cutaneous lymphoma (CL), two cases of actinic reticuloid as well as two cases of lymphocytic infiltration.Methods Immunohistochemistry studies were carried out on the infiltrati ng cells using monoclonal antibodies against CD45 RO, CD20, CD68 on paraffin e mbedded sections by ABC method and using monoclonal antibodies against CD3, CD4 and CD8 with indirect immunofluorescence (IIF) on frozen sections. Skin biopsies were examined by electron microscope. Serum and bone marrow cells were tested f or antibodies against HTLV Ⅰ associated antigen by IIF, and HTLV Ⅰ provirus DNA was examined by PCR method.Results The research showed four patients with ATLL manifesting cutaneo u s lesions, were subsequently found with additional systemic symptoms, as extensi vely enlarged superficial lymph node, abnormal increased IL 2 receptor, flower like cells in their peripheral blood and marrow. The HTLV Ⅰ provirus DNA was positi ve in the peripheral blood, bone marrow, cutaneous lesions and lymph node biopsy specimens by using PCR amplification of specific HTLV Ⅰ fragment while 18 cas es of the CL were negative for HTLV Ⅰ. The special ultrastructure of skin lesi ons was also found in ATLL patients.Conclusions The cutaneous involvement in ATLL is a type of cutaneous T cell lymphoma (CTCL) but shows some differential immunological markers for diffe rential diagnosis. The examination of HTLV Ⅰ antibodies or HTLV Ⅰ provirus D NA is necessary for diagnosis of ATLL. The ultrastrustural characteristics in skin lesions of ATLL were of atypic al lymphocytes and mononuclear cells invading the epidermis, and the mononuclear cells are possessing the phagocytic function and
基金the National Natural Science Foundation of China(Grant No.81401556 and 81601641)the Natural Science Foundation of Guangdong Province(No.2014A030310504 and 2014A030310293)+1 种基金the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry(No.2015-311)the Special Foundation of President of School of Public Health of Southern Medical University(Grant No.GW201619).
文摘The identification of antemortem burns and postmortem burns is essential in forensic practice.In this study,microRNA(miRNA)microarray analysis was conducted to identify differentially expressed miRNAs in the skin of an experimental burn model.Microarray analysis revealed 24 differentially expressed miRNAs in antemortem burned mice skin,with 19 miRNAs significantly upregulated and 5 downregulated.Based on the intersection predicted using three databases(Targetscan,microRNA.org,and PITA),293 potential miRNA targets were identified.These dysregulated miRNAs and their predicted targets were further analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases.Several functional categories and signaling pathways were enriched,including the“fc epsilon ri signaling pathway,”“endometrial cancer,”and“mTOR signaling pathway.”Expression patterns of 10 differentially expressed miRNAs were verified by reverse transcription‑quantitative polymerase chain reaction in mice skins.The results agreed with the results of microarray analysis.These findings suggest that differentially expressed miRNAs revealed by microarray are potential markers for forensic molecular diagnosis of antemortem burns.
文摘The sensitized fluorescence intensity of terbium ion can be notably enhanced when the Tb 3+ fleroxacin complex is exposed to 365 nm light. By the measurements of fluorescence spectra, phosphorescence spectra, fluorescence quantum yield and fluorescence lifetime of the system, it is proved that irradiation makes the complex undergo a photochemical reaction and produces a new terbium complex which is more favorable to intramolecular energy transfer. The mechanism of the photochemical fluorescence enhancement was discussed.
基金supported by National Key R&D Program of China(2017YFA0403402,2017YFA0700104)the National Natural Science Foundation of China(Grant Nos.11875258 and U1732232)+2 种基金the DNL Cooperation Fund,CAS(DNL180201)the Fundamental Research Funds for the Central Universities(Nos.WK2060190081)Users with Excellence Program of Hefei Science Center CAS(Nos.2018HSC-UE003 and 2019HSC-UE004)。
文摘Performance breakthrough of electrocatalysts highly relies on the regulation of internal structures and electronic states.In present work,for the first time,we successfully synthesized nitrogen doped FeS_(2) nanoparticles(N-FeS_(2))as the electrocatalysts for hydrogen evolution reaction(HER).The band structure and electronic state of FeS_(2) are modulated by a nitrogen doping strategy,as confirmed by X-ray photoelectron spectroscopy(XPS),X-ray absorption spectroscopy(XAS)and density functional theory(DFT)calculations.Owing to the band structure and electronic state regulation as well as the weakening of H-S interaction,the designed N-FeS_(2) electrocatalyst exhibits superior catalytic performance with a low overpotential(~126 mV at 10 mA cm^(-2))and excellent activity stability under alkaline conditions,which is substantially improved as compared with that of the pure FeS_(2) counterpart.Our work demonstrates that the modulation of electron state and band structure of an electrocatalyst,which can provide a valuable guidance for designing excellent catalysts for hydrogen evolution reaction and beyond.
文摘Developing highly efficient nickel or iron based hydroxide electrocatalysts is primary essential but challenging for oxygen evolution reaction(OER)at ultra-high current densities.Herein,we developed a facile method to prepare nitrogen and iron doped nickel(Ⅱ)hydroxide nanosheets on self-supported conductive nickel foam(denoted as Fe,N-Ni(OH)_(2)/NF)through ammonia hydrothermal and impregnation methods.Owing to the optimization of the electronic structure by nitrogen doping and the strong synergistic effect between Fe and Ni(OH)_(2),the three-dimensional(3 D)Fe,N-Ni(OH)_(2)/NF nanosheets delivered superior electrocatalytic OER performances in basic solution with low potentials of 1.57 V and1.59 V under 500 mA/cm^(2)and 1000 mA/cm^(2)respectively and robust operation for 10 h with ignored activity decay,comparing well with the potentials of previously reported NiFe based electrocatalysts as well as the benchmark commercial Ir/C/NF.In-situ Raman spectroscopy revealed that the main active species were NiOOH during the OER process.The present results are expected to provide new insights into the study of OER process towards ultra-high current densities.
基金the National Key R&D Program of China(Nos.2017YFA0208300,2017YFA0700104)the National Natural Science Foundtion of China(Nos.2522107,21671180,21521091,U1463202,21525313)+1 种基金the Fundamental Research Funds for the Central Universities,China(No.WK2060120004)the Postdoc Foundation Support,China(No.BH2060000111)。
文摘Searching for high-activity,stabilily and highly casbellective electrocalalysts for acid oxygen reaction rolutioo(ORR)has always been a urgent problem in polymer ectrolyte menbrane fuel ells(PEMFC).Nonetheless,the electrochemical poperties of various systems have their intrinsic limits and tremendous eforts have been paid oul to search for highly eficient electocatalysts by more raional control over the size,morphology,compoition,and structure.In particular,single-atom catalysts(SACs)have atrascted extensive intenest due to theirs cxcellant activity,stability,slctivity and the highest metal tiliztion In rceat yeurs,the number of papers in the field of SACs has incressed rapidly,indicating that SACs have made great progress.This review foouses on SACs electo-echemical applications in the acid ORR and introduces immovative syntheses,fiuel cell performance and long-time durabilily.
基金supported by the National Natural Science Foundation of China (No.22071211)the Science and Technology Planning Project of Hunan Province (No.2019RS2039)+1 种基金Hunan Provincial Natural Science Foundation of China (No.2020JJ3032)the Collaborative Innovation Center of New Chemical Technologies for Environmental Benignity and Efficient Resource Utilization。
文摘Visible-light-driven photochemical Cadogan-type cyclization has been discovered.The organic D-A type photosensitizer 4 CzIPN found to be an efficient mediator to transfer energy from photons to the transient intermediate that breaks the barriers of deoxygenation in Cadogan reaction and enables a mild metalfree access to carbazoles and related heterocycles.DFT calculation results indicate mildly endergonic formation of the intermediate complex of nitrobiarenes and PPh3,which corresponds with experimental findings regarding reaction temperature.The robust synthetic capacity of the photoredox Cadogan reaction systems has been demonstrated by the viable productivity of a broad range of carbazoles and related N-heterocycles with good tolerance of various functionalities.
