超高压(ultra high pressure,UHP)技术是一种将食品置于100 MPa以上的高压力下处理的新技术,可以改善食品品质和杀灭食品中的微生物。本文总结了国内外UHP乳制品加工领域的最新研究进展,介绍了UHP对原料乳、加工乳品品质的影响,重点阐述...超高压(ultra high pressure,UHP)技术是一种将食品置于100 MPa以上的高压力下处理的新技术,可以改善食品品质和杀灭食品中的微生物。本文总结了国内外UHP乳制品加工领域的最新研究进展,介绍了UHP对原料乳、加工乳品品质的影响,重点阐述了UHP在促进干酪成熟和低脂、低盐干酪生产等方面的优势,探讨了基于UHP乳制品加工的可行性与实效性,以期为UHP技术更好、更广泛地应用及改善乳制品品质提供理论基础。展开更多
Milk is one of the products that can be adulterated in many ways affecting the quality of this and its derivatives. Glucomacropeptide (GMP) is a protein that is found only in the whey from the production of fresh chee...Milk is one of the products that can be adulterated in many ways affecting the quality of this and its derivatives. Glucomacropeptide (GMP) is a protein that is found only in the whey from the production of fresh cheese, enzymatically obtained from the coagulation of casein and which is commonly used to adulterate fresh or powdered milk. The aim of this study was to determine the adulteration of milk with cheese whey thought a molecular approach, where the glucomacropeptide was collected by sequential precipitation with trichloroacetic acid (ATC) and detected by polyacrylamidododecylsulfate gel electrophoresis (PAGE-SDS), using samples of fresh milk, intentionally adulterated with serum in the proportion of 0%, 1%, 5%, 10% and 15%. The results obtained showed that the detection of glucomacropeptide by electrophoresis was positive in all samples of adulterated milk, evidencing a band of 20.9 kDa in the reading, corresponding to the molecular weight of the GMP, showing that the technique used determines the adulteration in the milk, in a specific and sensitive way, also shows that in the evaluation of physical-chemical and microbiological parameters of milk, there are no significant differences between treatments, except for the pH that tends to decrease as the percentage of serum in the milk increases.展开更多
文摘超高压(ultra high pressure,UHP)技术是一种将食品置于100 MPa以上的高压力下处理的新技术,可以改善食品品质和杀灭食品中的微生物。本文总结了国内外UHP乳制品加工领域的最新研究进展,介绍了UHP对原料乳、加工乳品品质的影响,重点阐述了UHP在促进干酪成熟和低脂、低盐干酪生产等方面的优势,探讨了基于UHP乳制品加工的可行性与实效性,以期为UHP技术更好、更广泛地应用及改善乳制品品质提供理论基础。
文摘Milk is one of the products that can be adulterated in many ways affecting the quality of this and its derivatives. Glucomacropeptide (GMP) is a protein that is found only in the whey from the production of fresh cheese, enzymatically obtained from the coagulation of casein and which is commonly used to adulterate fresh or powdered milk. The aim of this study was to determine the adulteration of milk with cheese whey thought a molecular approach, where the glucomacropeptide was collected by sequential precipitation with trichloroacetic acid (ATC) and detected by polyacrylamidododecylsulfate gel electrophoresis (PAGE-SDS), using samples of fresh milk, intentionally adulterated with serum in the proportion of 0%, 1%, 5%, 10% and 15%. The results obtained showed that the detection of glucomacropeptide by electrophoresis was positive in all samples of adulterated milk, evidencing a band of 20.9 kDa in the reading, corresponding to the molecular weight of the GMP, showing that the technique used determines the adulteration in the milk, in a specific and sensitive way, also shows that in the evaluation of physical-chemical and microbiological parameters of milk, there are no significant differences between treatments, except for the pH that tends to decrease as the percentage of serum in the milk increases.
