Quorum system is a preferable model to construct distributed access control architecture, but not all quorum system can satisfy the requirements of distributed access control architecture. Aiming at the dependable pro...Quorum system is a preferable model to construct distributed access control architecture, but not all quorum system can satisfy the requirements of distributed access control architecture. Aiming at the dependable problem of authorization server in distributed system and combining the requirements of access control, a set of criterions to select and evaluate quorum system is presented. The scheme and algorithm of constructing an authorization server system based on Paths quorum system are designed, and the integrated sys- tem performance under some servers attacked is fully analyzed. Role-based access control on the Web implemented by this scheme is introduced. Analysis shows that with certain node failure probability, the scheme not only has high dependability but also can satisfy the special requirements of distributed access control such as real-time, parallelism, and consistency of security policy.展开更多
Quorum systems have been used to solve the problem of data consistency in distributed fault-tolerance systems. But when intrusions occur, traditional quorum systems have some disadvantages. For example, synchronous qu...Quorum systems have been used to solve the problem of data consistency in distributed fault-tolerance systems. But when intrusions occur, traditional quorum systems have some disadvantages. For example, synchronous quorum systems are subject to DOS attacks, while asynchronous quorum systems need a larger system size （at least 3f＋1 for generic data, and f fewer for self-verifying data）. In order to solve the problems above, an intrusion-tolerance quorum system （ITQS） of hybrid time model based on trust timely computing base is presented （TTCB）. The TTCB is a trust secure real-time component inside the server with a well defined interface and separated from the operation system. It is in the synchronous communication environment while the application layer in the server deals with read-write requests and executes update-copy protocols asynchronously. The architectural hybridization of synchrony and asynchrony can achieve the data consistency and availability correctly. We also build two kinds of ITQSes based on TTCB, i.e., the symmetrical and the asymmetrical TTCB quorum systems. In the performance evaluations, we show that TTCB quorum systems are of smaller size, lower load and higher availability.展开更多
Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application ...Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality maintenance.Materials and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was verified.The growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/mL.Images of membrane integrity.SEM and biofilm formation suggested significant biological structure damage in bacteria after treatment.Meanwhile,the Qs signaling molecule Al-2 content showed a decline with increasing treatment concentration.Transcriptome sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying degree.Conclusion:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of strains.These synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.展开更多
Rapid developments in network systems of business service have resulted in more reliance on distributed computing, typified by “subscriber/push” architectures. Unfortunately, frequent and unexpectable network failur...Rapid developments in network systems of business service have resulted in more reliance on distributed computing, typified by “subscriber/push” architectures. Unfortunately, frequent and unexpectable network failures were routine, and downtime was not in hours, but in days. High availability has become the most important factor decreasing business risk and improving Quality of Service. Cluster technology has solved the non-stop problem on Local Area Network. However, most technologies including cluster today fail to ensure the non-stop Internet service based on Routers. With good performance on high availability and fault tolerance, quorum systems are very suitable for application to distributed business service networks. In this work, we modeled and developed a non-stop Internet service system based on a new quorum system, circle quorum system, for Boston Mutual Fund Broker, US. With five protocols, it provided highly available data services for clients on Internet.展开更多
Study of marine noctilucence in marine is important to fishery, environmental monitoring and military affairs. A luminous bacterial strain D2 was isolated from the marine sediment samples collected near Donghai Island...Study of marine noctilucence in marine is important to fishery, environmental monitoring and military affairs. A luminous bacterial strain D2 was isolated from the marine sediment samples collected near Donghai Island in Zhanjiang, China. The primary cultural and luminescent conditions of luminous bacterium D2 which was identified as Vibrio sp. were determined in liquid culture. The results showed that pH 7.0, 35 ℃, with 2.0 % NaCI, were the best growth conditions, and pH5 - 6, 20℃, OD600 0.08, with 3.0 % NaCI, were the optimal luminescent conditions.展开更多
基金Supported by the National Natural Science Foundation of China (70771043, 60873225, 60773191)
文摘Quorum system is a preferable model to construct distributed access control architecture, but not all quorum system can satisfy the requirements of distributed access control architecture. Aiming at the dependable problem of authorization server in distributed system and combining the requirements of access control, a set of criterions to select and evaluate quorum system is presented. The scheme and algorithm of constructing an authorization server system based on Paths quorum system are designed, and the integrated sys- tem performance under some servers attacked is fully analyzed. Role-based access control on the Web implemented by this scheme is introduced. Analysis shows that with certain node failure probability, the scheme not only has high dependability but also can satisfy the special requirements of distributed access control such as real-time, parallelism, and consistency of security policy.
基金supported by the National Natural Science Foundation of China (60774091)
文摘Quorum systems have been used to solve the problem of data consistency in distributed fault-tolerance systems. But when intrusions occur, traditional quorum systems have some disadvantages. For example, synchronous quorum systems are subject to DOS attacks, while asynchronous quorum systems need a larger system size （at least 3f＋1 for generic data, and f fewer for self-verifying data）. In order to solve the problems above, an intrusion-tolerance quorum system （ITQS） of hybrid time model based on trust timely computing base is presented （TTCB）. The TTCB is a trust secure real-time component inside the server with a well defined interface and separated from the operation system. It is in the synchronous communication environment while the application layer in the server deals with read-write requests and executes update-copy protocols asynchronously. The architectural hybridization of synchrony and asynchrony can achieve the data consistency and availability correctly. We also build two kinds of ITQSes based on TTCB, i.e., the symmetrical and the asymmetrical TTCB quorum systems. In the performance evaluations, we show that TTCB quorum systems are of smaller size, lower load and higher availability.
基金supported by the Hainan Provincial Natural Science Foundation of China (321CXTD1012)the National Natural Science Foundation of China (NSFC31871868)the Scientific Research Foundation of Hainan Tropical Ocean University (RHDRC202117),China.
文摘Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality maintenance.Materials and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was verified.The growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/mL.Images of membrane integrity.SEM and biofilm formation suggested significant biological structure damage in bacteria after treatment.Meanwhile,the Qs signaling molecule Al-2 content showed a decline with increasing treatment concentration.Transcriptome sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying degree.Conclusion:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of strains.These synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.
文摘Rapid developments in network systems of business service have resulted in more reliance on distributed computing, typified by “subscriber/push” architectures. Unfortunately, frequent and unexpectable network failures were routine, and downtime was not in hours, but in days. High availability has become the most important factor decreasing business risk and improving Quality of Service. Cluster technology has solved the non-stop problem on Local Area Network. However, most technologies including cluster today fail to ensure the non-stop Internet service based on Routers. With good performance on high availability and fault tolerance, quorum systems are very suitable for application to distributed business service networks. In this work, we modeled and developed a non-stop Internet service system based on a new quorum system, circle quorum system, for Boston Mutual Fund Broker, US. With five protocols, it provided highly available data services for clients on Internet.
文摘Study of marine noctilucence in marine is important to fishery, environmental monitoring and military affairs. A luminous bacterial strain D2 was isolated from the marine sediment samples collected near Donghai Island in Zhanjiang, China. The primary cultural and luminescent conditions of luminous bacterium D2 which was identified as Vibrio sp. were determined in liquid culture. The results showed that pH 7.0, 35 ℃, with 2.0 % NaCI, were the best growth conditions, and pH5 - 6, 20℃, OD600 0.08, with 3.0 % NaCI, were the optimal luminescent conditions.
