A bacterial strain,designated as LS,was isolated from a contaminated soil and was found to be capable of utilizing quinclorac,bensulfuronmethyl,and a mixture of the two as carbon and energy sources for growth. Strain ...A bacterial strain,designated as LS,was isolated from a contaminated soil and was found to be capable of utilizing quinclorac,bensulfuronmethyl,and a mixture of the two as carbon and energy sources for growth. Strain LS was identified as Ochrobactrum sp. based on its physiological-biochemical properties,16S rDNA sequences,and phylogenetic analysis. The extent of degradation of quinclorac and bensulfuronmethyl at initial concentrations of 1.5 and 0.1 g L-1 was 90% and 67%,respectively,as measured by high performance liquid chromatography(HPLC) . When a herbicide mixture of 0.34 g L-1 quinclorac and 0.02 g L-1 bensulfuronmethyl was applied as carbon sources,quinclorac and bensulfuronmethyl were degraded at 95.7% and 67.5%,respectively. It appears that quinclorac is utilized more easily in a mixture than in a single state. The optimal temperature for growth of strain LS was 37 ℃. Strain LS grew well at pH 6 to 9 and had the highest degradation level for quinclorac and bensulfuronmethyl at an initial pH of 7 and 8,respectively. Addition of 0.25 g L-1 yeast extract could promote the growth and extent of degradation of quinclorac and bensulfuronmethyl by strain LS. Strain LS also showed the capability to degrade other aromatic compounds such as catechol,propisochlor,4-chloro-2-methylphenoxyacetic acid sodium(MCPA-Na) and imazethapy. The isolate LS shows a huge potential to be used in bioremediation for treating complex herbicide residues.展开更多
【目的】为治理稻-烟轮作田块上茬土壤中二氯喹啉酸残留问题,筛选高效降解细菌菌株。【方法】通过富集培养和选择培养,从常年施用二氯喹啉酸的水稻田中筛选可以降解二氯喹啉酸的细菌;对其进行形态学观察、生理生化特征测定和16S r DNA...【目的】为治理稻-烟轮作田块上茬土壤中二氯喹啉酸残留问题,筛选高效降解细菌菌株。【方法】通过富集培养和选择培养,从常年施用二氯喹啉酸的水稻田中筛选可以降解二氯喹啉酸的细菌;对其进行形态学观察、生理生化特征测定和16S r DNA序列系统发育鉴定。【结果】分离的降解菌株MC-10被鉴定为节杆属菌株(Arthrobacter sp.)。菌株MC-10在5%接种量p H 7、28℃时,对初始浓度为20 mg/L二氯喹啉酸7 d可降解90%以上。该降解菌的最佳降解条件为p H 7、30℃,二氯喹啉酸初始浓度在1-100mg/L间均有良好的降解效果;菌株MC-10在土壤中对二氯喹啉酸同样有良好的降解效果,温室内7 d对二氯喹啉酸污染土壤的修复率可达70%。【结论】菌株MC-10在二氯喹啉酸污染土壤和水质治理中具有潜在的应用前景。展开更多
基金the National Natural Science Foundation of China (Nos.40501037 and 30570053)the National Key Technologies Research and Development Program of China during the 11th Five-Year Plan Period(No.2006BAJ08B01).
文摘A bacterial strain,designated as LS,was isolated from a contaminated soil and was found to be capable of utilizing quinclorac,bensulfuronmethyl,and a mixture of the two as carbon and energy sources for growth. Strain LS was identified as Ochrobactrum sp. based on its physiological-biochemical properties,16S rDNA sequences,and phylogenetic analysis. The extent of degradation of quinclorac and bensulfuronmethyl at initial concentrations of 1.5 and 0.1 g L-1 was 90% and 67%,respectively,as measured by high performance liquid chromatography(HPLC) . When a herbicide mixture of 0.34 g L-1 quinclorac and 0.02 g L-1 bensulfuronmethyl was applied as carbon sources,quinclorac and bensulfuronmethyl were degraded at 95.7% and 67.5%,respectively. It appears that quinclorac is utilized more easily in a mixture than in a single state. The optimal temperature for growth of strain LS was 37 ℃. Strain LS grew well at pH 6 to 9 and had the highest degradation level for quinclorac and bensulfuronmethyl at an initial pH of 7 and 8,respectively. Addition of 0.25 g L-1 yeast extract could promote the growth and extent of degradation of quinclorac and bensulfuronmethyl by strain LS. Strain LS also showed the capability to degrade other aromatic compounds such as catechol,propisochlor,4-chloro-2-methylphenoxyacetic acid sodium(MCPA-Na) and imazethapy. The isolate LS shows a huge potential to be used in bioremediation for treating complex herbicide residues.
文摘【目的】为治理稻-烟轮作田块上茬土壤中二氯喹啉酸残留问题,筛选高效降解细菌菌株。【方法】通过富集培养和选择培养,从常年施用二氯喹啉酸的水稻田中筛选可以降解二氯喹啉酸的细菌;对其进行形态学观察、生理生化特征测定和16S r DNA序列系统发育鉴定。【结果】分离的降解菌株MC-10被鉴定为节杆属菌株(Arthrobacter sp.)。菌株MC-10在5%接种量p H 7、28℃时,对初始浓度为20 mg/L二氯喹啉酸7 d可降解90%以上。该降解菌的最佳降解条件为p H 7、30℃,二氯喹啉酸初始浓度在1-100mg/L间均有良好的降解效果;菌株MC-10在土壤中对二氯喹啉酸同样有良好的降解效果,温室内7 d对二氯喹啉酸污染土壤的修复率可达70%。【结论】菌株MC-10在二氯喹啉酸污染土壤和水质治理中具有潜在的应用前景。
