The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models we...The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".展开更多
目的:观察王不留行子耳穴贴压联合益气补阴方治疗消渴病(2型糖尿病)气阴两虚证的临床疗效。方法:将气阴两虚证2型糖尿病患者120例依据随机数字表法分为观察组与对照组各60例。对照组采取常规治疗,观察组在对照组基础上给予王不留行子耳...目的:观察王不留行子耳穴贴压联合益气补阴方治疗消渴病(2型糖尿病)气阴两虚证的临床疗效。方法:将气阴两虚证2型糖尿病患者120例依据随机数字表法分为观察组与对照组各60例。对照组采取常规治疗,观察组在对照组基础上给予王不留行子耳穴贴压联合益气补阴方治疗。治疗12周后比较两组总有效率,治疗前后糖代谢[空腹血糖(fasting plasma glucose,FPG)、糖化血红蛋白(glycosylated hemo-globin,HbA1c)和餐后2 h血浆葡萄糖(2 hour plasma glucose,2hPG)]、主要中医症状、胰岛素抵抗指数(homeostasis model of insulin resistance index,HOMA-IR)和胰岛β细胞功能(HOMA-β)、血脂[高密度脂蛋白(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白(low density lipoprotein cho-lesterol,LDL-C)、甘油三酯(triglyceride,TG)和胆固醇(total cholesterol,TC)]变化情况及不良反应。结果:总有效率观察组为91.67%,高于对照组的73.33%(P<0.05)。观察组治疗后咽干口燥、倦怠乏力积分分别低于对照组(P<0.05)。观察组治疗后FPG、HbA1c及2hPG分别低于对照组(P<0.05)。观察组治疗后HOMA-IR低于对照组,HOMA-β高于对照组(P<0.05)。观察组治疗后HDL-C高于对照组,而LDL-C、TG及TC分别低于对照组(P<0.05)。两组均未出现明显不良反应。结论:王不留行子耳穴贴压联合益气补阴方可改善2型糖尿病患者糖代谢,降低HOMA-IR,提高HOMA-β,改善血脂紊乱,效果优于常规治疗。展开更多
基金Supported by the National Natural Science Foundation of China (Grant Nos 30630076 and 30572228)the National 11th 5 Year Technology Support Program (Grant No 2006BAI08B04-05)
文摘The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".
文摘目的:观察王不留行子耳穴贴压联合益气补阴方治疗消渴病(2型糖尿病)气阴两虚证的临床疗效。方法:将气阴两虚证2型糖尿病患者120例依据随机数字表法分为观察组与对照组各60例。对照组采取常规治疗,观察组在对照组基础上给予王不留行子耳穴贴压联合益气补阴方治疗。治疗12周后比较两组总有效率,治疗前后糖代谢[空腹血糖(fasting plasma glucose,FPG)、糖化血红蛋白(glycosylated hemo-globin,HbA1c)和餐后2 h血浆葡萄糖(2 hour plasma glucose,2hPG)]、主要中医症状、胰岛素抵抗指数(homeostasis model of insulin resistance index,HOMA-IR)和胰岛β细胞功能(HOMA-β)、血脂[高密度脂蛋白(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白(low density lipoprotein cho-lesterol,LDL-C)、甘油三酯(triglyceride,TG)和胆固醇(total cholesterol,TC)]变化情况及不良反应。结果:总有效率观察组为91.67%,高于对照组的73.33%(P<0.05)。观察组治疗后咽干口燥、倦怠乏力积分分别低于对照组(P<0.05)。观察组治疗后FPG、HbA1c及2hPG分别低于对照组(P<0.05)。观察组治疗后HOMA-IR低于对照组,HOMA-β高于对照组(P<0.05)。观察组治疗后HDL-C高于对照组,而LDL-C、TG及TC分别低于对照组(P<0.05)。两组均未出现明显不良反应。结论:王不留行子耳穴贴压联合益气补阴方可改善2型糖尿病患者糖代谢,降低HOMA-IR,提高HOMA-β,改善血脂紊乱,效果优于常规治疗。
