Soybean mosaic virus （SMV） is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, Rsc4, Rsc8, and Rsc14Q, have been identified and mapped on soybean chromosomes 14, 2, and...Soybean mosaic virus （SMV） is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, Rsc4, Rsc8, and Rsc14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. Soybean cultivar Nannong 1138-2 is widely grown in the Yangtze River Valley of China. In this study, crosses were made between Qihuang l^Kefeng 1 and DabaimaxNannong 1138-2. Ten simple sequence repeat （SSR） markers linked to three resistance loci （Rsc4, Rsc8, and Rsc^4Q） were used to assist pyramided breeding. Pyramided families containing three resistance loci （Rsc4, Rsc8, and Rsc14Q） were evaluated by inoculating them with 21 SMV strains from China. Results indicated that the 10 markers can be used effectively to assist the selection of resistant individuals containing Rsc4, Rsc8, and Rsc14Q. A total of 53 F6 plants were confirmed to contain three homozygous alleles conferring resistance to SMV. Five F7 homozygous pyramided families exhibited resistance to 21 strains of SMV and showed desirable agronomic traits using dual selection. The strategy of pyramiding resistance gene derived from different varieties has practical breeding value in providing broad-spectrum resistance against the existing strains of SMV in China.展开更多
The reactions of rice bacterial leaf blight races were identified in Guilan province—Iran on 12 near-isogenic lines and 14 pyramiding lines from International Network for Genetic Evaluation of rice (INGER) and 8 loca...The reactions of rice bacterial leaf blight races were identified in Guilan province—Iran on 12 near-isogenic lines and 14 pyramiding lines from International Network for Genetic Evaluation of rice (INGER) and 8 local and improved Iranian varieties were evaluated under natural photoperiod condition in the field. Inoculation was done at panicle initiation by clipping the sterilized scissors in the bacterial suspension to booting stage. Scoring of inoculated plants was made 21 days after inoculation. Infection levels of pyramiding lines containing two to five resistance genes, expect, IRBB53 and IRBB61 with respectively resistance gene combination, Xa5 + Xa13 and Xa4 + Xa5 + Xa7, were not so clear. Among near-isogenic lines IRBB1, IRBB2, IRBB4 and IRBB10 carrying resistance gene Xa1, Xa2, Xa4 and Xa10 were susceptible;IRBB8, IRBB11, IRBB3, IRBB5 and IRBB13 were moderately susceptible;(having resistance gene Xa8, Xa11, Xa3, Xa5 and Xa13) IRBB14, IRBB21 and IRBB7 with respectively resistance gene Xa14, Xa21 and Xa7 were moderately resistance to bacterial blight. Furthermore, most of the time gene combinations support the strategy of pyramiding appropriate resistance gene. Local varieties were more susceptible than improved varieties to leaf blight disease. Among local varieties, Tarom was the most susceptible. And also, there were no significant differences among improved varieties and all of them were moderately resistance.展开更多
基金supported by the National Natural Science Foundation of China(31571687,31571690,and 31371646)the Natural Science Foundation of Anhui Province,China(1708085MC69)+1 种基金the Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)the Fund of Transgenic Breeding for Soybean Resistance to Soybean Mosaic Virus,China(2016ZX08004-004)
文摘Soybean mosaic virus （SMV） is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, Rsc4, Rsc8, and Rsc14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. Soybean cultivar Nannong 1138-2 is widely grown in the Yangtze River Valley of China. In this study, crosses were made between Qihuang l^Kefeng 1 and DabaimaxNannong 1138-2. Ten simple sequence repeat （SSR） markers linked to three resistance loci （Rsc4, Rsc8, and Rsc^4Q） were used to assist pyramided breeding. Pyramided families containing three resistance loci （Rsc4, Rsc8, and Rsc14Q） were evaluated by inoculating them with 21 SMV strains from China. Results indicated that the 10 markers can be used effectively to assist the selection of resistant individuals containing Rsc4, Rsc8, and Rsc14Q. A total of 53 F6 plants were confirmed to contain three homozygous alleles conferring resistance to SMV. Five F7 homozygous pyramided families exhibited resistance to 21 strains of SMV and showed desirable agronomic traits using dual selection. The strategy of pyramiding resistance gene derived from different varieties has practical breeding value in providing broad-spectrum resistance against the existing strains of SMV in China.
文摘The reactions of rice bacterial leaf blight races were identified in Guilan province—Iran on 12 near-isogenic lines and 14 pyramiding lines from International Network for Genetic Evaluation of rice (INGER) and 8 local and improved Iranian varieties were evaluated under natural photoperiod condition in the field. Inoculation was done at panicle initiation by clipping the sterilized scissors in the bacterial suspension to booting stage. Scoring of inoculated plants was made 21 days after inoculation. Infection levels of pyramiding lines containing two to five resistance genes, expect, IRBB53 and IRBB61 with respectively resistance gene combination, Xa5 + Xa13 and Xa4 + Xa5 + Xa7, were not so clear. Among near-isogenic lines IRBB1, IRBB2, IRBB4 and IRBB10 carrying resistance gene Xa1, Xa2, Xa4 and Xa10 were susceptible;IRBB8, IRBB11, IRBB3, IRBB5 and IRBB13 were moderately susceptible;(having resistance gene Xa8, Xa11, Xa3, Xa5 and Xa13) IRBB14, IRBB21 and IRBB7 with respectively resistance gene Xa14, Xa21 and Xa7 were moderately resistance to bacterial blight. Furthermore, most of the time gene combinations support the strategy of pyramiding appropriate resistance gene. Local varieties were more susceptible than improved varieties to leaf blight disease. Among local varieties, Tarom was the most susceptible. And also, there were no significant differences among improved varieties and all of them were moderately resistance.
