以宜兴百合为原料,采用超声辅助热水法提取百合多糖。百合粗多糖经Sevag脱蛋白,透析后用DEAESepharose Fast Flow及Sephadex G-100色谱柱纯化,得到百合多糖组分LLPS-1、LLPS-2和LLPS-3。经考马斯亮蓝、紫外光谱及高效凝胶过滤色谱(HPGFC...以宜兴百合为原料,采用超声辅助热水法提取百合多糖。百合粗多糖经Sevag脱蛋白,透析后用DEAESepharose Fast Flow及Sephadex G-100色谱柱纯化,得到百合多糖组分LLPS-1、LLPS-2和LLPS-3。经考马斯亮蓝、紫外光谱及高效凝胶过滤色谱(HPGFC)检测,3种百合多糖不含蛋白及核酸,具有较高纯度。经HPGFC测定,3种百合多糖的重均分子质量分别是350.5、403.3kD和146.2kD。展开更多
This manuscript presents a dataset detailing a method for purifying monomers. Purification plays a crucial role in every chemical process, as it leads to an improvement in product quality through the removal of impuri...This manuscript presents a dataset detailing a method for purifying monomers. Purification plays a crucial role in every chemical process, as it leads to an improvement in product quality through the removal of impurities. The primary method for monomer purification, like acrylonitrile (AN), is the distillation technique. However, this technique is unsafe and hard to set up or handle. A straightforward, risk-free, low-cost method like the column technique resolves these issues. A simple column technique demonstrated the successful execution of purifying AN. Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses confirmed that AN was successfully purified, with purity reaching 99.8%. FTIR spectra revealed changes in the position and intensity of the stretching vibration peaks after purification. Also, the functional groups of the inhibitor monomethyl ether of hydroquinone (MeHQ) were undetected after purification. Furthermore, after purification, NMR spectra revealed the absence of aromatic protons and carbons associated with MeHQ. In conclusion, the column technique is a successful and inexpensive way to purify AN monomers. This makes it useful for a wide range of applications, especially in polymerization reactions where MeHQ needs to be removed to prevent self-polymerization during the initiation process.展开更多
文摘以宜兴百合为原料,采用超声辅助热水法提取百合多糖。百合粗多糖经Sevag脱蛋白,透析后用DEAESepharose Fast Flow及Sephadex G-100色谱柱纯化,得到百合多糖组分LLPS-1、LLPS-2和LLPS-3。经考马斯亮蓝、紫外光谱及高效凝胶过滤色谱(HPGFC)检测,3种百合多糖不含蛋白及核酸,具有较高纯度。经HPGFC测定,3种百合多糖的重均分子质量分别是350.5、403.3kD和146.2kD。
文摘This manuscript presents a dataset detailing a method for purifying monomers. Purification plays a crucial role in every chemical process, as it leads to an improvement in product quality through the removal of impurities. The primary method for monomer purification, like acrylonitrile (AN), is the distillation technique. However, this technique is unsafe and hard to set up or handle. A straightforward, risk-free, low-cost method like the column technique resolves these issues. A simple column technique demonstrated the successful execution of purifying AN. Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analyses confirmed that AN was successfully purified, with purity reaching 99.8%. FTIR spectra revealed changes in the position and intensity of the stretching vibration peaks after purification. Also, the functional groups of the inhibitor monomethyl ether of hydroquinone (MeHQ) were undetected after purification. Furthermore, after purification, NMR spectra revealed the absence of aromatic protons and carbons associated with MeHQ. In conclusion, the column technique is a successful and inexpensive way to purify AN monomers. This makes it useful for a wide range of applications, especially in polymerization reactions where MeHQ needs to be removed to prevent self-polymerization during the initiation process.
