猪繁殖与呼吸障碍综合征(porcine reproductive and respiratory syndrome,PRRS)是由猪繁殖与呼吸障碍综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)引发的高急性、高度接触性、高致死传染性疾病,也被称为猪...猪繁殖与呼吸障碍综合征(porcine reproductive and respiratory syndrome,PRRS)是由猪繁殖与呼吸障碍综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)引发的高急性、高度接触性、高致死传染性疾病,也被称为猪蓝耳病。临床上表现为发烧、咳嗽、耳朵发紫、呼吸困难等症状,会导致母猪繁殖性障碍、流产、死胎,仔猪呼吸系统疾病,生产实践中死亡率极高。目前尚无有效的特异性药物能对其治疗,本文从蓝耳病的发病原因、传播途径、临床症状、病理变化、实验室诊断、综合防治措施进行阐述,以期能为蓝耳病的诊断和防治提供帮助。展开更多
Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating disease with worldwide distribution caused by Betaarterivirus suid (PRRSV). The virion has great genetic and antigenic variability wi...Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating disease with worldwide distribution caused by Betaarterivirus suid (PRRSV). The virion has great genetic and antigenic variability with a marked increase in virulence. Vaccines tested to date have been of little use in controlling the problems caused by PRRSV, so the present study was conceived to evaluate the antiviral effect of polymeric nanoparticles (PNPs) made with glycyrrhizic acid (GA). Recent work has proven that this nanoparticle system is stable. These nanoparticles have good GA carrying capacity, a size < 250 nm, a spherical morphology, and a wide safety range. The integrity of cell morphology can be maintained for up to 72 h. The antiviral effect of this nanoparticle system was tested in cultures of MARC-145 cells in pre- and coinfection assays with PRRSV to evaluate changes in cell morphology and effects on cell viability. The use of PNPsGA with the real-time quantitative polymerase chain reaction (RT-qPCR) decreased viral infection by 38% in 3 amplification cycles. These results suggest that this system has an antiviral effect against PRRSV under the study conditions established.展开更多
文摘猪繁殖与呼吸障碍综合征(porcine reproductive and respiratory syndrome,PRRS)是由猪繁殖与呼吸障碍综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)引发的高急性、高度接触性、高致死传染性疾病,也被称为猪蓝耳病。临床上表现为发烧、咳嗽、耳朵发紫、呼吸困难等症状,会导致母猪繁殖性障碍、流产、死胎,仔猪呼吸系统疾病,生产实践中死亡率极高。目前尚无有效的特异性药物能对其治疗,本文从蓝耳病的发病原因、传播途径、临床症状、病理变化、实验室诊断、综合防治措施进行阐述,以期能为蓝耳病的诊断和防治提供帮助。
文摘Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating disease with worldwide distribution caused by Betaarterivirus suid (PRRSV). The virion has great genetic and antigenic variability with a marked increase in virulence. Vaccines tested to date have been of little use in controlling the problems caused by PRRSV, so the present study was conceived to evaluate the antiviral effect of polymeric nanoparticles (PNPs) made with glycyrrhizic acid (GA). Recent work has proven that this nanoparticle system is stable. These nanoparticles have good GA carrying capacity, a size < 250 nm, a spherical morphology, and a wide safety range. The integrity of cell morphology can be maintained for up to 72 h. The antiviral effect of this nanoparticle system was tested in cultures of MARC-145 cells in pre- and coinfection assays with PRRSV to evaluate changes in cell morphology and effects on cell viability. The use of PNPsGA with the real-time quantitative polymerase chain reaction (RT-qPCR) decreased viral infection by 38% in 3 amplification cycles. These results suggest that this system has an antiviral effect against PRRSV under the study conditions established.
