AIM:To investigate and elucidate the molecular mechanism underlying varioliform gastritis for early detection,prevention and intervention of gastric cancer.METHODS:A combination of two-dimensional gel electrophoresis ...AIM:To investigate and elucidate the molecular mechanism underlying varioliform gastritis for early detection,prevention and intervention of gastric cancer.METHODS:A combination of two-dimensional gel electrophoresis and mass spectrometry was used to detect the differentially expressed proteins between varioliform gastritis and matched normal mucosa.The selected proteins were confirmed by Western blotting and reverse transcription polymerase chain reaction(RT-PCR) in additional samples and the function of some proteins in varioliform gastritis was analyzed by bio-method preliminarily.RESULTS:We identified 21 differentially expressed proteins in varioliform gastritis,and compared them with matched normal mucosa.Eleven proteins were upregulated and ten downregulated in varioliform gastritis when compared with the same proteins in individualmatched normal gastric mucosa.These proteins are related to metabolism,oxidation,cytoskeleton,apoptosis,signal transduction and other aspects of cells.Two novel proteins,thioredoxin domain-containing protein 5(TXNDC5) upregulated in varioliform gastritis,and neuropolypeptide h3 [phosphatidylethanolamine-binding protein 1(PEBP1)] downregulated in varioliform gastritis,were further investigated.Their expressions were validated by Western blotting and RT-PCR in 12 cases of varioliform gastritis which was matched with normal mucosa.The expression level of PEBP1 in varioliform gastritis was significantly lower(P<0.05) while that of TXNDC5 was significantly higher than that in matched normal gastric mucosa(P<0.05).CONCLUSION:There are some changes of protein expression in varioliform gastritis.Downregulation of PEBP1 and upregulation of TXNDC5 are involved in the development of varioliform gastritis.展开更多
As an important second messenger, calcium is involved in plant cold stress response, including chilling（〈20 °C） and freezing（〈0 °C）. In this study, exogenous application of calcium chloride（CaCl2） im...As an important second messenger, calcium is involved in plant cold stress response, including chilling（〈20 °C） and freezing（〈0 °C）. In this study, exogenous application of calcium chloride（CaCl2） improved both chilling and freezing stress tolerances, while ethylene glycol‐bis‐（b‐aminoethyl） ether‐N,N,N,N‐tetraacetic acid（EGTA） reversed CaCl2 effects in bermudagrass（Cynodon dactylon（L.） Pers.）.Pysiological analyses showed that CaCl2 treatment alleviated the reactive oxygen species（ROS） burst and cell damage triggered by chilling stress, via activating antioxidant enzymes,non‐enzymatic glutathione antioxidant pool, while EGTA treatment had the opposite effects. Additionally, comparative proteomic analysis identified 51 differentially expressed proteins that were enriched in redox, tricarboxylicacid cycle,glycolysis, photosynthesis, oxidative pentose phosphate pathway, and amino acid metabolisms. Consistently, 42 metabolites including amino acids, organic acids, sugars, and sugar alcohols were regulated by CaCl2 treatment under control and cold stress conditions, further confirming the Researchcommon modulation of CaCl2 treatment in carbon metabolites and amino acid metabolism. Taken together, this study reported first evidence of the essential and protective roles of endogenous and exogenous calcium in bermudagrass response to cold stress, partially via activation of the antioxidants and modulation of several differentially expressed proteins and metabolic homeostasis in the process of cold acclimation.展开更多
Nasopharyngeal carcinoma （NPC） is rare in most countries, especially in Europe and NorthAmerica （incidence rate below 1/100 000 people per year）. However, it has a high incidence in several southern areas in China...Nasopharyngeal carcinoma （NPC） is rare in most countries, especially in Europe and NorthAmerica （incidence rate below 1/100 000 people per year）. However, it has a high incidence in several southern areas in China, especially in the Cantonese region, including Guangzhou city, where the incidence rate is approximately 30-80/100 000 people per year. In the high incidence areas, more than 95% NPCs are poorly differentiated （WHO types 2 and 3）, which are sensitive to radiotherapy and chemotherapy. Besides its special epidemiological and pathological characteristics,展开更多
Over recent decades, many studies have reported that hypocrellin A(HA) can eliminate cancer cells with proper irradiation in several cancer cell lines. However, the precise molecular mechanism underlying its anticance...Over recent decades, many studies have reported that hypocrellin A(HA) can eliminate cancer cells with proper irradiation in several cancer cell lines. However, the precise molecular mechanism underlying its anticancer effect has not been fully defined. HA-mediated cytotoxicity and apoptosis in human lung adenocarcinoma A549 cells were evaluated after photodynamic therapy(PDT). A temporal quantitative proteomics approach by isobaric tag for relative and absolute quantitation(iTRAQ) 2 D liquid chromatography with tandem mass spectrometric(LC–MS/MS) was introduced to help clarify molecular cytotoxic mechanisms and identify candidate targets of HA-induced apoptotic cell death. Specific caspaseinhibitors were used to further elucidate the molecular pathway underlying apoptosis in PDT-treated A549 cells. Finally, down-stream apoptosis-related protein was evaluated. Apoptosis induced by HA was associated with cell shrinkage, externalization of cell membrane phosphatidylserine, DNA fragmentation,and mitochondrial disruption, which were preceded by increased intracellular reactive oxygen species(ROS) generations. Further studies showed that PDT treatment with 0.08 mmol/L HA resulted in mitochondrial disruption, pronounced release of cytochrome c, and activation of caspase-3,-9, and-7.Together, HA may be a possible therapeutic agent directed toward mitochondria and a promising photodynamic anticancer candidate for further evaluation.展开更多
Colorectal cancer (CRC) is still one of the leading causes of cancer-related death in Western countries, despite major improvements in its treatment. The dramatically high social and economic impact of CRC on human he...Colorectal cancer (CRC) is still one of the leading causes of cancer-related death in Western countries, despite major improvements in its treatment. The dramatically high social and economic impact of CRC on human health makes the identification of a reliable screening tool of paramount importance. Current screening methods, such as the fecal occult blood test and colonoscopy do not adequately meet the ideal requisites of a screening test because, even if they are effective, they are limited first by too low specificity and sensitivity, or second by high invasiveness, costs and risk. Nowadays extended efforts are made by researchers to look for more reliable and effective screening tests based on a systems biology approach, using biological samples easily available, such as urine, breath, serum and feces. The effectiveness and reliability of several new attempts to screen these patients by non-invasive analysis of their biological samples using genomic (genetic and epigenetic alteration), transcriptomic (miRNA), proteomic (cancer-related antigens, new antibodies against tumor-associated antigens, mutated proteins) and metabolomic (volatile organic metabolites) methods are discussed in this review. Among the most interesting new screening tools, fecal fluorescent long-DNA, fecal miRNA and metabolomic evaluation in breath and/ or serum seem to be most promising.展开更多
This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chron...This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and progression.SK-OV-3 cells were injected subcutaneously into nude mice.The stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days,while the control group was unrestrained.All mice were sacrificed on day 36 after SK-OV-3 cell injection,and tumors were excised.Tumor tissues were processed for 2D gel electrophoresis,mass spectrometry(nanoUPLC-ESI-MS/MS) and Western blotting.The expression of 20 proteins was found to be significantly altered between the stress and control groups,of which 14 were up-regulated,five were down-regulated,and one protein was found only in the stress group.All proteins were identified by UPLC-ESI-MS/MS,and Western blotting results were consistent with those of proteomic methods.The present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression.展开更多
文摘AIM:To investigate and elucidate the molecular mechanism underlying varioliform gastritis for early detection,prevention and intervention of gastric cancer.METHODS:A combination of two-dimensional gel electrophoresis and mass spectrometry was used to detect the differentially expressed proteins between varioliform gastritis and matched normal mucosa.The selected proteins were confirmed by Western blotting and reverse transcription polymerase chain reaction(RT-PCR) in additional samples and the function of some proteins in varioliform gastritis was analyzed by bio-method preliminarily.RESULTS:We identified 21 differentially expressed proteins in varioliform gastritis,and compared them with matched normal mucosa.Eleven proteins were upregulated and ten downregulated in varioliform gastritis when compared with the same proteins in individualmatched normal gastric mucosa.These proteins are related to metabolism,oxidation,cytoskeleton,apoptosis,signal transduction and other aspects of cells.Two novel proteins,thioredoxin domain-containing protein 5(TXNDC5) upregulated in varioliform gastritis,and neuropolypeptide h3 [phosphatidylethanolamine-binding protein 1(PEBP1)] downregulated in varioliform gastritis,were further investigated.Their expressions were validated by Western blotting and RT-PCR in 12 cases of varioliform gastritis which was matched with normal mucosa.The expression level of PEBP1 in varioliform gastritis was significantly lower(P<0.05) while that of TXNDC5 was significantly higher than that in matched normal gastric mucosa(P<0.05).CONCLUSION:There are some changes of protein expression in varioliform gastritis.Downregulation of PEBP1 and upregulation of TXNDC5 are involved in the development of varioliform gastritis.
基金supported by "the Hundred Talents Program," the Knowledge Innovative Key Program of the Chinese Academy of Sciences (54Y154761O01076 and Y329631O0263) to Z.C.the National Natural Science Foundation of China (31200194)+1 种基金the outstanding young talent program of Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture (Y352811O03)Youth Innovation Promotion Association of the Chinese Academy of Sciences to H.S.
文摘As an important second messenger, calcium is involved in plant cold stress response, including chilling（〈20 °C） and freezing（〈0 °C）. In this study, exogenous application of calcium chloride（CaCl2） improved both chilling and freezing stress tolerances, while ethylene glycol‐bis‐（b‐aminoethyl） ether‐N,N,N,N‐tetraacetic acid（EGTA） reversed CaCl2 effects in bermudagrass（Cynodon dactylon（L.） Pers.）.Pysiological analyses showed that CaCl2 treatment alleviated the reactive oxygen species（ROS） burst and cell damage triggered by chilling stress, via activating antioxidant enzymes,non‐enzymatic glutathione antioxidant pool, while EGTA treatment had the opposite effects. Additionally, comparative proteomic analysis identified 51 differentially expressed proteins that were enriched in redox, tricarboxylicacid cycle,glycolysis, photosynthesis, oxidative pentose phosphate pathway, and amino acid metabolisms. Consistently, 42 metabolites including amino acids, organic acids, sugars, and sugar alcohols were regulated by CaCl2 treatment under control and cold stress conditions, further confirming the Researchcommon modulation of CaCl2 treatment in carbon metabolites and amino acid metabolism. Taken together, this study reported first evidence of the essential and protective roles of endogenous and exogenous calcium in bermudagrass response to cold stress, partially via activation of the antioxidants and modulation of several differentially expressed proteins and metabolic homeostasis in the process of cold acclimation.
基金This work was supported by the Guangdong Social Developed Projectof Science and Technology (No.2003-245)
文摘Nasopharyngeal carcinoma （NPC） is rare in most countries, especially in Europe and NorthAmerica （incidence rate below 1/100 000 people per year）. However, it has a high incidence in several southern areas in China, especially in the Cantonese region, including Guangzhou city, where the incidence rate is approximately 30-80/100 000 people per year. In the high incidence areas, more than 95% NPCs are poorly differentiated （WHO types 2 and 3）, which are sensitive to radiotherapy and chemotherapy. Besides its special epidemiological and pathological characteristics,
基金supported by the National Natural Science Foundation of China(Project No.81673214)The National Key Technology Research and National Key Technology Research and Development Program of the Ministry of Science and Technology of the People's Republic of China(Project No.2012BAD36B0502)the Priority Academic Program Development of Jiangsu Higher Educational Institutions(China)
文摘Over recent decades, many studies have reported that hypocrellin A(HA) can eliminate cancer cells with proper irradiation in several cancer cell lines. However, the precise molecular mechanism underlying its anticancer effect has not been fully defined. HA-mediated cytotoxicity and apoptosis in human lung adenocarcinoma A549 cells were evaluated after photodynamic therapy(PDT). A temporal quantitative proteomics approach by isobaric tag for relative and absolute quantitation(iTRAQ) 2 D liquid chromatography with tandem mass spectrometric(LC–MS/MS) was introduced to help clarify molecular cytotoxic mechanisms and identify candidate targets of HA-induced apoptotic cell death. Specific caspaseinhibitors were used to further elucidate the molecular pathway underlying apoptosis in PDT-treated A549 cells. Finally, down-stream apoptosis-related protein was evaluated. Apoptosis induced by HA was associated with cell shrinkage, externalization of cell membrane phosphatidylserine, DNA fragmentation,and mitochondrial disruption, which were preceded by increased intracellular reactive oxygen species(ROS) generations. Further studies showed that PDT treatment with 0.08 mmol/L HA resulted in mitochondrial disruption, pronounced release of cytochrome c, and activation of caspase-3,-9, and-7.Together, HA may be a possible therapeutic agent directed toward mitochondria and a promising photodynamic anticancer candidate for further evaluation.
文摘Colorectal cancer (CRC) is still one of the leading causes of cancer-related death in Western countries, despite major improvements in its treatment. The dramatically high social and economic impact of CRC on human health makes the identification of a reliable screening tool of paramount importance. Current screening methods, such as the fecal occult blood test and colonoscopy do not adequately meet the ideal requisites of a screening test because, even if they are effective, they are limited first by too low specificity and sensitivity, or second by high invasiveness, costs and risk. Nowadays extended efforts are made by researchers to look for more reliable and effective screening tests based on a systems biology approach, using biological samples easily available, such as urine, breath, serum and feces. The effectiveness and reliability of several new attempts to screen these patients by non-invasive analysis of their biological samples using genomic (genetic and epigenetic alteration), transcriptomic (miRNA), proteomic (cancer-related antigens, new antibodies against tumor-associated antigens, mutated proteins) and metabolomic (volatile organic metabolites) methods are discussed in this review. Among the most interesting new screening tools, fecal fluorescent long-DNA, fecal miRNA and metabolomic evaluation in breath and/ or serum seem to be most promising.
基金supported by the National Natural Science Foundation of China (Grant Nos. 30860301 and 30660192)
文摘This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chronic psychological stress to ovarian cancer oncogenesis and progression.SK-OV-3 cells were injected subcutaneously into nude mice.The stress group was subjected to a chronic physical restraint protocol for 6 h on 35 consecutive days,while the control group was unrestrained.All mice were sacrificed on day 36 after SK-OV-3 cell injection,and tumors were excised.Tumor tissues were processed for 2D gel electrophoresis,mass spectrometry(nanoUPLC-ESI-MS/MS) and Western blotting.The expression of 20 proteins was found to be significantly altered between the stress and control groups,of which 14 were up-regulated,five were down-regulated,and one protein was found only in the stress group.All proteins were identified by UPLC-ESI-MS/MS,and Western blotting results were consistent with those of proteomic methods.The present results provide new evidence relating to the molecular mechanism underlying the relationship between psychological stress and tumor progression.
