期刊文献+
共找到200篇文章
< 1 2 10 >
每页显示 20 50 100
不同转移潜能人肝癌细胞系转录因子活性差异分析 被引量:21
1
作者 潘奇 王鲁 +3 位作者 孙惠川 刘银坤 叶胜龙 汤钊猷 《中华肝脏病杂志》 CAS CSCD 北大核心 2006年第1期37-40,共4页
目的分析不同转移潜能人肝癌细胞系细胞核内转录因子活性的差异,筛选与肝癌转移相关的转录因子。方法应用转录因子活性芯片技术,在功能水平分析三种不同转移潜能人肝癌细胞系(Hep3B、 MHCC97L和MHCC97H)细胞核内转录因子活性的差异,并... 目的分析不同转移潜能人肝癌细胞系细胞核内转录因子活性的差异,筛选与肝癌转移相关的转录因子。方法应用转录因子活性芯片技术,在功能水平分析三种不同转移潜能人肝癌细胞系(Hep3B、 MHCC97L和MHCC97H)细胞核内转录因子活性的差异,并用电泳迁移率变动分析和蛋白免疫印迹实验验证芯片结果。结果在345个候选的转录因子中,筛选出7个活性差异转录因子。随人肝癌细胞转移潜能的增高(Hep3B<MHCC97L<MHCC97H),活性上调的转录因子有5个,包括p53、缺氧诱导因子-1α(HIF- 1α)、核因子k b、信号传导及转录活化因子3(stat3)和Spl;活性下调的转录因子有2个,包括Rb和Smad3。结论转录因子活性异常与肝癌转移密切相关,本实验筛选出的转录因子可能有助于揭示肝癌转移的分子机制,并寻找新的预测指标及干预治疗的靶点。 展开更多
关键词 肝细胞 肿瘤转移 转录因子 蛋白质芯片 DNA芯片 细胞株
原文传递
Topical application of glycyrrhizin preparation ameliorates experimentally induced colitis in rats 被引量:23
2
作者 Tomohiro Kudo Shinichi Okamura +2 位作者 Yajing Zhang Takashige Masuo Masatomo Mori 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第17期2223-2228,共6页
AIM:To examine the efficacy of glycyrrhizin preparation(GL-p) in the treatment of a rat model of ulcerative colitis(UC).METHODS:Experimental colitis was induced by oral administration of dextran sodium sulfate.Rats wi... AIM:To examine the efficacy of glycyrrhizin preparation(GL-p) in the treatment of a rat model of ulcerative colitis(UC).METHODS:Experimental colitis was induced by oral administration of dextran sodium sulfate.Rats with colitis were intrarectally administered GL-p or saline.The extent of colitis was evaluated based on body weight gain,colon wet weight,and macroscopic damage score.The expression levels of pro-inflammatory cytokines and chemokines in the inflamed mucosa were measured by cytokine antibody array analysis.The effect of GL-p on myeloperoxidase(MPO) activity in the inflamed mucosa and purified enzyme was assayed.RESULTS:GL-p treatment significantly ameliorated the extent of colitis compared to sham treatment with saline.Cytokine antibody array analysis showed that GL-p treatment significantly decreased the expression levels of pro-inflammatory cytokines and chemokines,including interleukin(IL)-1β,IL-6,tumor necrosis factor-α,cytokine-induced neutrophil chemoattractant-2,and monocyte chemoattractant protein-1 in the inflamed mucosa.Furthermore,GL-p inhibited the oxidative activity of mucosal and purified MPO.CONCLUSION:GL-p enema has a therapeutic effect on experimental colitis in rats and may be useful in the treatment of UC. 展开更多
关键词 GLYCYRRHIZIN COLITIS Dextran sodium sulfate Ulcerative colitis Cytokine CHEMOKINE protein array MYELOPEROXIDASE ENEMA CARBOXYMETHYLCELLULOSE
下载PDF
体外培养的肝癌细胞株与正常肝细胞株蛋白质的差异表达 被引量:11
3
作者 宋旭霞 闫志勇 +4 位作者 王斌 牟文凤 钱冬萌 丁守怡 姚宗良 《世界华人消化杂志》 CAS 北大核心 2005年第22期2689-2692,共4页
目的:运用SELDI蛋白质芯片技术分析体外培养的肝癌细胞株(HepG2)与正常肝细胞株(L02)蛋白质表达差异.方法:在体外培养HepG2和L02细胞株,收获细胞,将细胞用细胞裂解液裂解后,采用SELDI蛋白质芯片技术用IMAC3 及WCX2芯片检测HepG2、L02... 目的:运用SELDI蛋白质芯片技术分析体外培养的肝癌细胞株(HepG2)与正常肝细胞株(L02)蛋白质表达差异.方法:在体外培养HepG2和L02细胞株,收获细胞,将细胞用细胞裂解液裂解后,采用SELDI蛋白质芯片技术用IMAC3 及WCX2芯片检测HepG2、L02的蛋白质谱.结果:体外培养的肝癌细胞株与正常肝细胞株的蛋白质存在差异表达,IMAC3芯片共捕获61个蛋白,发现差异峰7个,与 L02细胞相比,其中3个差异蛋白在肝癌细胞中高表达,4个差异蛋白在肝癌细胞中低表达.WCX2芯片共捕获91个蛋白, 发现差异峰14个,其中3个差异蛋白在肝癌细胞中高表达,11 个差异蛋白在肝癌细胞中低表达.结论:SELDI蛋白芯片技术检测肝癌细胞株与正常肝细胞株蛋白质的差异表达方法简便,敏感性高,重复性好. 展开更多
关键词 蛋白质芯片 肝癌细胞株 差异蛋白
下载PDF
应用蛋白质芯片检测骨性关节炎患者基质金属蛋白酶-13的初步研究 被引量:11
4
作者 班吉鹤 周利武 +3 位作者 毛广平 郭亭 唐祖明 赵建宁 《医学研究生学报》 CAS 2008年第8期836-838,844,I0002,共5页
目的:探讨应用蛋白质芯片技术检测骨性关节炎(OA)患者关节液和血清中基质金属蛋白酶-13(MMP-13)的方法。方法:应用低密度蛋白质芯片方法同时检测32例OA患者关节液和血清中MMP-13表达水平,24例正常关节作为对照组,进行蛋白质芯片荧光强... 目的:探讨应用蛋白质芯片技术检测骨性关节炎(OA)患者关节液和血清中基质金属蛋白酶-13(MMP-13)的方法。方法:应用低密度蛋白质芯片方法同时检测32例OA患者关节液和血清中MMP-13表达水平,24例正常关节作为对照组,进行蛋白质芯片荧光强度的对比研究。结果:OA患者关节液和血清中的MMP-13在蛋白质芯片上的荧光强度明显高于对照组关节液的荧光强度(P<0.001)。OA患者关节液中MMP-13在蛋白质芯片上的荧光强度明显高于其血清的荧光强度(P<0.001)。结论:OA患者关节液和血清中MMP-13表达明显高于对照组。应用低密度的蛋白质芯片技术检测OA患者关节液及血清中MMP-13的水平具有一定临床应用价值。 展开更多
关键词 蛋白质芯片 骨性关节炎 基质金属蛋白酶-13
下载PDF
蛋白质与抗体微阵列及其在生物医学研究中的应用 被引量:8
5
作者 虞伟 孙永康 +1 位作者 顾宁 武建国 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2002年第3期491-494,共4页
随着人类基因组测序的顺利完成及其他相关领域如机械制造、微电子加工技术及生物信息学方面所取得的进展 ,以蛋白质为研究对象的蛋白质组学愈显重要 ,高通量的蛋白质与抗体阵列芯片分析技术正日益为人们关注 .对蛋白质分析策略及以阵列... 随着人类基因组测序的顺利完成及其他相关领域如机械制造、微电子加工技术及生物信息学方面所取得的进展 ,以蛋白质为研究对象的蛋白质组学愈显重要 ,高通量的蛋白质与抗体阵列芯片分析技术正日益为人们关注 .对蛋白质分析策略及以阵列为基础的蛋白质芯片分析原理、相关的制备方法与检测技术及其在生物学研究、医学与实验诊断应用方面进行了阐述 。 展开更多
关键词 蛋白质 抗体微阵列 生物医学研究 应用
下载PDF
门静脉高压症脾功能亢进与正常脾组织细胞因子表达差异的研究 被引量:7
6
作者 马双余 李爱民 +5 位作者 李宗芳 张澍 蒋安 张健 周蕊 党双锁 《中华普通外科学文献(电子版)》 2008年第6期24-27,共4页
目的研究门静脉高压症(portal hypertension,PH)脾亢脾与正常脾组织细胞因子的表达差异,为进一步探讨PH脾亢的发生机制和脾亢脾的免疫功能奠定基础。方法正常脾组织取自脾外伤手术切除的脾脏(A组,6例),脾亢脾组织取自PH脾亢患者手术切... 目的研究门静脉高压症(portal hypertension,PH)脾亢脾与正常脾组织细胞因子的表达差异,为进一步探讨PH脾亢的发生机制和脾亢脾的免疫功能奠定基础。方法正常脾组织取自脾外伤手术切除的脾脏(A组,6例),脾亢脾组织取自PH脾亢患者手术切除的脾脏(B组,14例);无菌取材后提取两组脾组织的蛋白质,用RayBio Human Cytokine Array5.1细胞因子芯片(美国Ray生物技术公司)同时检测两组脾组织79个细胞因子的表达,得到两组细胞因子表达点阵图,用ScanAlyze图像分析软件对两组间的细胞因子表达差异进行统计学分析。结果79个细胞因子中,共有26个在两组间的表达呈现明显差异。在PH脾亢脾组织中表达上调的细胞因子共有21个,其中包括与单核细胞趋化和巨噬细胞活化相关的细胞因子如MCSF、TNF-β、IFN-γ、IL-10和SDF-1/CXCL12等,以及与血管新生、纤维化和组织改建相关的细胞因子如VEGF、TGF-β1、MIF、FGF-9、Flt-3、Ligand和IGFBP-2等。脾亢脾组织表达下调的细胞因子共有5个。结论PH脾亢脾与正常脾组织细胞因子存在明显表达差异,这些差异表达的细胞因子可能是导致PH脾脏的免疫功能变化及脾亢发生的重要因素之一,但尚需进一步研究证实。 展开更多
关键词 门静脉高压症 脾功能亢进 细胞因子 蛋白质芯片
原文传递
应用蛋白质芯片检测多药耐药蛋白表达水平 被引量:2
7
作者 陈宝安 杜鹃 +3 位作者 张春秀 程坚 高峰 陆祖宏 《中华肿瘤杂志》 CAS CSCD 北大核心 2005年第9期528-530,共3页
目的探讨蛋白质芯片在检测白血病细胞多药耐药(MDR)蛋白表达中的价值。方法以人红白血病细胞系K562及其耐药细胞系K562/A02为实验研究对象。将3种耐药蛋白P糖蛋白(P-gP)、多药耐药相关蛋白(MRP1)和乳腺癌耐药蛋白(BCRP)相应的单克隆抗... 目的探讨蛋白质芯片在检测白血病细胞多药耐药(MDR)蛋白表达中的价值。方法以人红白血病细胞系K562及其耐药细胞系K562/A02为实验研究对象。将3种耐药蛋白P糖蛋白(P-gP)、多药耐药相关蛋白(MRP1)和乳腺癌耐药蛋白(BCRP)相应的单克隆抗体固定在玻片上形成微阵列,细胞直接与固定在芯片上的耐药抗体阵列反应,电荷耦合器件(CCD)检测反应结果,并与流式细胞术测定的结果进行比较分析。结果在K562细胞中,蛋白质芯片检测到P-gP和BCRP表达率低,MRP1有较高水平的表达;在K562/A02细胞中,P-gP和MRP1均有高水平表达,BCRP表达率低。流式细胞术结果显示,K562细胞P-gP、MRP1和BCRP表达率分别为5.98%±2.19%、95.80%±3.98%和1.03%±0.45%;K562/A02细胞P-gP、MRP1和BCRP表达率分别为92.67%±1.80%、97.18%±1.02%和3.98%±0.37%。经统计学分析,两种方法结果一致(Ρ>0.05)。结论利用蛋白质芯片检测MDR蛋白结果可靠,具有高通量、低成本、制备简单、测定快速的优点。 展开更多
关键词 蛋白质芯片 多药耐药蛋白 红白血病细胞系K562 多药耐药(MDR) 蛋白表达水平 芯片检测 K562/A02细胞 蛋白(P-gP) 人红白血病细胞系 乳腺癌耐药蛋白
原文传递
蛋白质芯片在功能蛋白组学研究中的应用 被引量:4
8
作者 杜宗敏 杨瑞馥 《军事医学科学院院刊》 CSCD 北大核心 2005年第1期72-76,83,共6页
 蛋白质芯片技术的应用使得对数以千计的蛋白质进行高通量、平行分析成为可能。蛋白质芯片是一种固定了保持天然活性的蛋白质微阵列,广义上,将能与蛋白质特异性结合的DNA和RNA、糖类、合成多肽,其他能够从复杂的混合物中特异性地捕获...  蛋白质芯片技术的应用使得对数以千计的蛋白质进行高通量、平行分析成为可能。蛋白质芯片是一种固定了保持天然活性的蛋白质微阵列,广义上,将能与蛋白质特异性结合的DNA和RNA、糖类、合成多肽,其他能够从复杂的混合物中特异性地捕获目的蛋白的小分子物质的微阵列也称为蛋白质芯片。蛋白质芯片可以检测感染性疾病、自身免疫性疾病及肿瘤等患者体内的疾病标志分子,还能研究蛋白质的功能、与其他蛋白质之间的相互作用、蛋白表达谱等蛋白质组学研究所涵盖的内容。本文将重点阐述蛋白质芯片在功能蛋白质组学研究中的应用进展。 展开更多
关键词 蛋白质芯片 功能蛋白组学
原文传递
Dip-pen刻蚀技术直接制造蛋白质纳米阵列 被引量:4
9
作者 魏莉 洪霞 +2 位作者 郭薇 白玉白 李铁津 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2002年第7期1386-1388,共3页
This paper covers the first application of Dip Pen Nanolithography(DPN) to directly write protein patterns with submicrometer dimensions onto Au substrate. Using Bovine Serum Albumin(BSA) as the ink in the DPN procedu... This paper covers the first application of Dip Pen Nanolithography(DPN) to directly write protein patterns with submicrometer dimensions onto Au substrate. Using Bovine Serum Albumin(BSA) as the ink in the DPN procedure, we were able to utilize lateral force microscopy(LFM) images to differentiate between Au substrate and patterned area with deposited monolayers of BSA. Then the first evidence for Au_S bonding was reported between the gold substrate and the BSA surface thiol groups given by the angle resolved XPS measurements. 展开更多
关键词 纳米阵列 Dip-pen纳米刻蚀 协同作用力 蛋白质 生物芯片 金属氧化物
下载PDF
Storage time affects the level and diagnostic efficacy of plasma biomarkers for neurodegenerative diseases
10
作者 Lifang Zhao Mingkai Zhang +4 位作者 Qimeng Li Xuemin Wang Jie Lu Ying Han Yanning Cai 《Neural Regeneration Research》 SCIE CAS 2025年第8期2373-2381,共9页
Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative diseases.However,little is k... Several promising plasma biomarker proteins,such as amyloid-β(Aβ),tau,neurofilament light chain,and glial fibrillary acidic protein,are widely used for the diagnosis of neurodegenerative diseases.However,little is known about the long-term stability of these biomarker proteins in plasma samples stored at-80°C.We aimed to explore how storage time would affect the diagnostic accuracy of these biomarkers using a large cohort.Plasma samples from 229 cognitively unimpaired individuals,encompassing healthy controls and those experiencing subjective cognitive decline,as well as 99 patients with cognitive impairment,comprising those with mild cognitive impairment and dementia,were acquired from the Sino Longitudinal Study on Cognitive Decline project.These samples were stored at-80°C for up to 6 years before being used in this study.Our results showed that plasma levels of Aβ42,Aβ40,neurofilament light chain,and glial fibrillary acidic protein were not significantly correlated with sample storage time.However,the level of total tau showed a negative correlation with sample storage time.Notably,in individuals without cognitive impairment,plasma levels of total protein and tau phosphorylated protein threonine 181(p-tau181)also showed a negative correlation with sample storage time.This was not observed in individuals with cognitive impairment.Consequently,we speculate that the diagnostic accuracy of plasma p-tau181 and the p-tau181 to total tau ratio may be influenced by sample storage time.Therefore,caution is advised when using these plasma biomarkers for the identification of neurodegenerative diseases,such as Alzheimer's disease.Furthermore,in cohort studies,it is important to consider the impact of storage time on the overall results. 展开更多
关键词 Alzheimer’s disease amyloid-β diagnostic ability glial fibrillary acidic protein NEURODEGENERATION neurofilament light chain plasma biomarkers single molecule array storage time tau
Development of a competitive array for discriminative determination of amphenicols in egg based on ribosomal protein L16
11
作者 才艺 马宁 +1 位作者 吴宁鹏 王建平 《Food Quality and Safety》 SCIE CAS CSCD 2024年第1期105-114,共10页
Objective:Amphenicols(chloramphenicol,thiamphenicol and forfenicol)can cause aplastic anaemia and other severe side effects to consumers;therefore,it is necessary to inspect their residues in foods of animal origin.Ho... Objective:Amphenicols(chloramphenicol,thiamphenicol and forfenicol)can cause aplastic anaemia and other severe side effects to consumers;therefore,it is necessary to inspect their residues in foods of animal origin.However,there has been no report on the use of amphenicols receptor for the determination of their residues,and none of the previously reported immunoassays for amphenicols can differentiate the specifc species.Materials and Methods:In this study,the ribosomal protein L16 of Escherichia coli was frst expressed,and its intermolecular interaction mechanisms with the three amphenicols was studied using the molecular docking technique.The protein was then combined with three enzymelabelled conjugates to develop a direct competitive array on microplate for determination of the three drugs in egg.Results:Due to the use of principal component analysis to analyse the data,this method could discriminate the three drugs in the range 0.1–10 ng/mL,and the limits of detection for the three drugs were in the range of 0.0002–0.0009 ng/mL.The analysis results for the unknown egg samples were consistent with a liquid chromatography–tandem mass spectrometry method,and the method performances were superior to the previous immunoassays for amphenicols.Conclusion:This is the frst paper reporting the use of ribosomal protein L16 to develop a competitive array for discriminative determination of amphenicols in food samples. 展开更多
关键词 Amphenicols ribosomal protein L16 recognition mechanism competitive array discriminative determination egg.
原文传递
Protein Array-based Approaches for Biomarker Discovery in Cancer 被引量:4
12
作者 Yi Huang Heng Zhu 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2017年第2期73-81,共9页
Biomarkers are deemed to be potential tools in early diagnosis, therapeutic monitoring,and prognosis evaluation for cancer, with simplicity as well as economic advantages compared with computed tomography and biopsy. ... Biomarkers are deemed to be potential tools in early diagnosis, therapeutic monitoring,and prognosis evaluation for cancer, with simplicity as well as economic advantages compared with computed tomography and biopsy. However, most of the current cancer biomarkers present insufficient sensitivity as well as specificity. Therefore, there is urgent requirement for the discovery of biomarkers for cancer. As one of the most exciting emerging technologies, protein array provides a versatile and robust platform in cancer proteomics research because it shows tremendous advantages of miniaturized features, high throughput, and sensitive detections in last decades. Here, we will present a relatively complete picture on the characteristics and advance of different types of protein arrays in application for biomarker discovery in cancer, and give the future perspectives in this area of research. 展开更多
关键词 protein array BIOMARKER CANCER PROTEOMICS Early diagnosis
原文传递
Changes in proteins related to early nerve repair in a rat model of sciatic nerve injury 被引量:5
13
作者 Yu-Song Yuan Fei Yu +3 位作者 Ya-Jun Zhang Su-Ping Niu Hai-Lin Xu Yu-Hui Kou 《Neural Regeneration Research》 SCIE CAS CSCD 2021年第8期1622-1627,共6页
Peripheral nerves have a limited capacity for self-repair and those that are severely damaged or have significant defects are challenging to repair. Investigating the pathophysiology of peripheral nerve repair is impo... Peripheral nerves have a limited capacity for self-repair and those that are severely damaged or have significant defects are challenging to repair. Investigating the pathophysiology of peripheral nerve repair is important for the clinical treatment of peripheral nerve repair and regeneration. In this study, rat models of right sciatic nerve injury were established by a clamping method. Protein chip assay was performed to quantify the levels of neurotrophic, inflammation-related, chemotaxis-related and cell generation-related factors in the sciatic nerve within 7 days after injury. The results revealed that the expression levels of neurotrophic factors(ciliary neurotrophic factor) and inflammationrelated factors(intercellular cell adhesion molecule-1, interferon γ, interleukin-1α, interleukin-2, interleukin-4, interleukin-6, monocyte chemoattractant protein-1, prolactin R, receptor of advanced glycation end products and tumor necrosis factor-α), chemotaxis-related factors(cytokine-induced neutrophil chemoattractant-1, L-selectin and platelet-derived growth factor-AA) and cell generation-related factors(granulocyte-macrophage colony-stimulating factor) followed different trajectories. These findings will help clarify the pathophysiology of sciatic nerve injury repair and develop clinical treatments of peripheral nerve injury. This study was approved by the Ethics Committee of Peking University People's Hospital of China(approval No. 2015-50) on December 9, 2015. 展开更多
关键词 animal model cell generation CHEMOTAXIS clamp injury INFLAMMATION INJURY neurotrophic factor peripheral nerve protein array repair sciatic nerve Wallerian degeneration
下载PDF
蛋白质芯片技术应用于高通量单克隆抗体制备研究 被引量:3
14
作者 宋凯 叶赛 +6 位作者 周佳菁 彭海林 王升年 卫玲 肖华胜 赵国屏 张庆华 《生物工程学报》 CAS CSCD 北大核心 2007年第6期1116-1120,共5页
针对在传统的单克隆抗体制备过程中进行特异性筛选时大量的人力消耗,建立了一种联合应用蛋白质芯片进行单克隆抗体制备的方法。用8种重组蛋白分别免疫BALB/c小鼠,在传统的细胞融合的基础上,将8种抗原免疫的杂交瘤阳性细胞混合后进行克... 针对在传统的单克隆抗体制备过程中进行特异性筛选时大量的人力消耗,建立了一种联合应用蛋白质芯片进行单克隆抗体制备的方法。用8种重组蛋白分别免疫BALB/c小鼠,在传统的细胞融合的基础上,将8种抗原免疫的杂交瘤阳性细胞混合后进行克隆化、蛋白质芯片筛选,阳性细胞有限稀释克隆化制备相关抗体。实验结果:混合克隆化共得到单克隆细胞175孔,经蛋白质芯片筛选出阳性孔119孔,选择针对单一抗原阳性的细胞连续2轮克隆化,8种重组蛋白各获得单克隆抗体细胞株1株。与经典的单克隆抗体制备相比,蛋白质芯片筛选与混合克隆化技术联合应用于单克隆抗体制备,1个筛选周期获得了8种重组蛋白的单克隆抗体细胞株,提高了单克隆抗体的制备效率,节省了在筛选中的抗原用量,提供了一种经济、快速、简便的方法。 展开更多
关键词 蛋白质芯片 单克隆抗体 克隆化 筛选
下载PDF
抗体芯片分析炎性细胞因子在严重脓毒症中的表达 被引量:3
15
作者 严静 张召才 +3 位作者 蔡国龙 虞意华 胡才宝 吴亮 《中华急诊医学杂志》 CAS CSCD 2006年第9期830-833,共4页
目的应用抗体芯片分析严重脓毒症患者血清中炎性细胞因子的表达。方法12例符合严重脓毒症诊断标准的患者和10例年龄、性别匹配的对照组患者入选。用生物素标记从两组患者血清中提取的总蛋白,然后与预先点有40个主要炎症相关细胞因子的... 目的应用抗体芯片分析严重脓毒症患者血清中炎性细胞因子的表达。方法12例符合严重脓毒症诊断标准的患者和10例年龄、性别匹配的对照组患者入选。用生物素标记从两组患者血清中提取的总蛋白,然后与预先点有40个主要炎症相关细胞因子的特异抗体的抗体芯片膜反应,目标蛋白与辣根过氧化物酶(HRP)标记的抗链霉生物素抗体结合并曝光显示反应信号,最后用激光扫描仪将其转化为图像文件进行分析。结果与对照组相比,脓毒症患者组血清中多种炎性细胞因子包括促炎因子、抗炎因子、趋化因子、某些细胞因子受体等的表达水平大幅度提高,但抗炎因子白介素(IL)-2、-4、-13、-15表达明显下降。结论严重脓毒症病程中存在炎症反应过度、抗炎因子与促炎因子失衡。 展开更多
关键词 抗体芯片 脓毒症 细胞因子 炎症 蛋白芯片
原文传递
Proteomic composition of the acrostyle" Novel approaches to identify cuticular proteins involved in virus-insect interactions 被引量:3
16
作者 Craig Graham Webster Maelle Thillier +3 位作者 Elodie Pirolles Bastien Cayrol Stephane Blanc Marilyne Uzest 《Insect Science》 SCIE CAS CSCD 2017年第6期990-1002,共13页
The acrostyle is a distinct anatomical region present on the cuticle at the inner face of the common food/salivary canal at the tip of aphid maxillary stylets. This conserved structure is of particular interest as it ... The acrostyle is a distinct anatomical region present on the cuticle at the inner face of the common food/salivary canal at the tip of aphid maxillary stylets. This conserved structure is of particular interest as it harbors the protein receptors of at least l plant virus, Cauliflower mosaic virus', and presumably has other roles in plant-insect interactions. Previously we reported immunolabeling of a highly conserved motif of cuticular proteins from the CPR family (named for the presence of a Rebers and Riddiford consensus) within the acrostyle. Here we report the development of novel tools to further study the proteomic composition of this region and to identify proteins involved in insect- virus interactions. Using a series of antibodies against cuticular proteins from the RR-2 subfamily, we identified additional peptides present within the acrostyle. Our results demonstrated that the acrostyle is a complex structure containing multiple domains of cuticular proteins accessible for interaction. In addition, an array of overlapping peptides,which covers the diversity of the majority of the RR-2 subfamily, was ~tevelopect as a generic tool to characterize cuticular protein/pathogen interactions. Upon probing this array with Cucumber mosaic virus particles, consensus peptide sequences from hybridizing peptides were identified. Use of these novel tools has extended our knowledge of the proteomic composition of insect maxillary stylets and identified sequences that could be involved in virus binding, thus contributing to further elucidation of the various properties and functions of the acrostyle. 展开更多
关键词 acrostyle APHID cuticular protein IMMUNOLABELING peptide array stylet
原文传递
Methyl-CpG-Binding protein 2 duplication syndrome in a Chinese patient:A case report and review of the literature
17
作者 Xu-Hang Xing Russel Takam +2 位作者 Xiu-Ying Bao Nour Abdallah Ba-alwi Hong Ji 《World Journal of Clinical Cases》 SCIE 2023年第27期6505-6514,共10页
BACKGROUND Chromosomal Xq28 region duplication encompassing methyl-CpG-binding protein 2(MECP2)results in an identifiable phenotype and global developmental delay known as MECP2 duplication syndrome(MDS).This syndrome... BACKGROUND Chromosomal Xq28 region duplication encompassing methyl-CpG-binding protein 2(MECP2)results in an identifiable phenotype and global developmental delay known as MECP2 duplication syndrome(MDS).This syndrome has a wide range of clinical manifestations,including abnormalities in appearance,neurodevelopment,and gastrointestinal motility;recurrent infections;and spasticity.Here,we report a case of confirmed MDS at our institution.CASE SUMMARY A 12-year-old Chinese boy presented with intellectual disability(poor intellectual[reasoning,judgment,abstract thinking,and learning]and adaptive[lack of communication and absent social skills,apraxia,and ataxia]functioning)and dysmorphism.He had no history of recurrent infections,seizures,or bowel dysfunction,which is different from that in reported cases.Microarray comparative genomic hybridization confirmed MECP2 duplication in the patient and his mother who is a carrier.The duplication size was the same in the patient and his mother.No prophylactic antibiotic or anti-seizure therapy was offered to the patient or his mother before or after the consultation.CONCLUSION MDS is rare and has various clinical presentations.Clinical suspicion is critical in patients presenting with developmental delays. 展开更多
关键词 Methyl-CpG-binding protein 2 Neurodevelopmental Delay Xq28 duplication array comparative genomic hybridization Case report
下载PDF
基因Ⅳ型戊型肝炎病毒ORF3对靶细胞脂肪因子表达的影响 被引量:2
18
作者 杜丽 成鹰 +7 位作者 史巧芸 焦寒伟 荣辉 张珈宁 贾晓晓 郭莳雨 朱华培 王凤阳 《动物医学进展》 CSCD 北大核心 2013年第6期78-80,共3页
为了探讨戊型肝炎病毒ORF3影响靶细胞脂肪因子表达的分子机制,应用蛋白芯片技术比较稳定表达EGFP-ORF3的HEK293细胞和稳定表达EGFP的HEK293细胞的脂肪因子表达谱,筛选获得显著上调/下调的脂肪因子。结果表明,在稳定表达EGFP-ORF3的HEK29... 为了探讨戊型肝炎病毒ORF3影响靶细胞脂肪因子表达的分子机制,应用蛋白芯片技术比较稳定表达EGFP-ORF3的HEK293细胞和稳定表达EGFP的HEK293细胞的脂肪因子表达谱,筛选获得显著上调/下调的脂肪因子。结果表明,在稳定表达EGFP-ORF3的HEK293细胞,Fas和TIMP-2的表达水平升高,而ACE-2表达水平降低。研究结果为HEV ORF3对于靶细胞重要脂肪因子表达影响的分子机制研究提供了重要依据。 展开更多
关键词 蛋白芯片 戊型肝炎病毒 靶细胞 脂肪因子 表达
下载PDF
一种蛋白质阵列芯片新技术的研究 被引量:1
19
作者 韩欢欢 于晓波 +2 位作者 吕任极 何为 许丹科 《分析试验室》 CAS CSCD 北大核心 2008年第2期107-110,共4页
将微印刷技术与银增强法相结合,建立了一种蛋白质阵列芯片制备与检测的新方法。通过定量分析证明了蛋白质可以被均匀地转印至固相载体表面,采用银增强法对蛋白质的直接法检测和夹心法检测的最低检测量分别可达0.33和0.13 fmol。此新方... 将微印刷技术与银增强法相结合,建立了一种蛋白质阵列芯片制备与检测的新方法。通过定量分析证明了蛋白质可以被均匀地转印至固相载体表面,采用银增强法对蛋白质的直接法检测和夹心法检测的最低检测量分别可达0.33和0.13 fmol。此新方法应用于蛋白质阵列上具有灵活、灵敏及成本低廉等特点。 展开更多
关键词 蛋白阵列芯片 微印刷技术 银增强检测
下载PDF
基因Ⅳ型戊型肝炎病毒 ORF3对 HEK293细胞 MAPKs磷酸化的影响 被引量:2
20
作者 杜丽 成鹰 +9 位作者 史巧芸 焦寒伟 荣辉 张珈宁 贾晓晓 郭莳雨 朱华培 赵天靖 徐开莲 王凤阳 《动物医学进展》 CSCD 北大核心 2014年第4期46-48,共3页
为了分析戊型肝炎病毒ORF3蛋白对于靶细胞MAPK磷酸化的影响,应用人磷酸化MAPK抗体微阵列比较稳定表达EGFP-ORF3的HEK293细胞和稳定表达EGFP的HEK293细胞的磷酸化MAPK表达谱,筛选获得显著上调/下调的磷酸化MAPK。结果表明,在稳定表达EGFP... 为了分析戊型肝炎病毒ORF3蛋白对于靶细胞MAPK磷酸化的影响,应用人磷酸化MAPK抗体微阵列比较稳定表达EGFP-ORF3的HEK293细胞和稳定表达EGFP的HEK293细胞的磷酸化MAPK表达谱,筛选获得显著上调/下调的磷酸化MAPK。结果表明,在稳定表达EGFP-ORF3的HEK293细胞,p-Akt2、p-Aktpan、p-ERK1、p-ERF2、p-RSK1的表达水平升高,而p-P38б表达水平降低。结果表明,ORF3蛋白影响了HEK293细胞的p-Akt2、p-Aktpan、p-ERK1、p-ERF2、p-RSK1、p-P38б等MAPKs的磷酸化水平,为HEV ORF3对于靶细胞磷酸化MAPK表达影响的分子机制研究提供了重要依据。 展开更多
关键词 蛋白芯片 戊型肝炎病毒 靶细胞 MAPK磷酸化 表达
下载PDF
上一页 1 2 10 下一页 到第
使用帮助 返回顶部