利用A—PAGE（Acid—polyacrylamide gel electrophoresis）技术对来源于32个国家的53份红花材料进行了种子醇溶蛋白检测。结果表明，这些红花材料具有丰富的醇溶蛋白等位变异，共分离出15条迁移率不同的谱带。其中，每份材料可电泳出5...利用A—PAGE（Acid—polyacrylamide gel electrophoresis）技术对来源于32个国家的53份红花材料进行了种子醇溶蛋白检测。结果表明，这些红花材料具有丰富的醇溶蛋白等位变异，共分离出15条迁移率不同的谱带。其中，每份材料可电泳出5-12条谱带，平均8．5条，所有材料有1条共有带。红花种子醇溶蛋白可作为评价红花遗传多样性的工具之一。不同材料的遗传相似系数（GS）变异范围为0．375—1．000，平均值为0．752。聚类分析结果表明，在GS值为0．752的水平上供试材料聚为6大类，其亲缘关系远近与地理来源关系不大。其中，来自中国的7份材料分别被聚在了3个大类中，表明中国红花种子醇溶蛋白遗传多样性比较丰富。展开更多
利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明，油用型红花醇溶蛋白位点存在丰富的变异类型，共分离出15条迁移率不同的谱带，每份材料具有3—12条...利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明，油用型红花醇溶蛋白位点存在丰富的变异类型，共分离出15条迁移率不同的谱带，每份材料具有3—12条不等，平均8．5条。材料间平均遗传相似系数（GS）为0．5303，变幅为0．1333—1．000，且各洲间遗传多样性大于洲内遗传多样性。在GS值为0．512的水平上，供试材料聚为六大类，聚类结果表明，红花醇溶蛋白图谱类型与其地理分布有一定的相关，但不显著。展开更多
Rice(Oryza sativa L.)is the most important food crop for at least half of the world’s population.Due to improved living standards,the cultivation of high-quality rice for different purposes and markets has become a m...Rice(Oryza sativa L.)is the most important food crop for at least half of the world’s population.Due to improved living standards,the cultivation of high-quality rice for different purposes and markets has become a major goal.Rice quality is determined by the presence of many nutritional components,including seed storage proteins(SSPs),which are the second most abundant nutrient components of rice grains after starch.Rice SSP biosynthesis requires the participation of multiple organelles and is influenced by the external environment,making it challenging to understand the molecular details of SSP biosynthesis and improve rice protein quality.In this review,we highlight the current knowledge of rice SSP biosynthesis,including a detailed description of the key molecules involved in rice SSP biosynthetic processes and the major environmental factors affecting SSP biosynthesis.The effects of these factors on SSP accumulation and their contribution to rice quality are also discussed based on recent findings.This recent knowledge suggests not only new research directions for exploring rice SSP biosynthesis but also innovative strategies for breeding high-quality rice varieties.展开更多
为了考察红花的种内变异,为进一步进行红花种质资源选育奠定基础。利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于中国不同地区的19份红花材料醇溶蛋白位点进行检测。结果表明,红花种子醇溶蛋白位点存在丰富的变...为了考察红花的种内变异,为进一步进行红花种质资源选育奠定基础。利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于中国不同地区的19份红花材料醇溶蛋白位点进行检测。结果表明,红花种子醇溶蛋白位点存在丰富的变异类型,共分离出13条迁移率不同的谱带,每份材料具有8-11条不等,平均10条。材料间平均遗传相似系数（GS）为0.759,变幅为0.385-1.000。在GS值为0.25的水平上,供试材料聚为两大类,聚类结果表明,红花醇溶蛋白图谱类型与其地理分布有很大的相关性。展开更多
Prolamins from thirteen kinds of homemade rice seed have been preliminary stu-dled by capillary zone electrophoresis(CZE). The results showed that CZE can be used as asupplement method for comparing and differentiatin...Prolamins from thirteen kinds of homemade rice seed have been preliminary stu-dled by capillary zone electrophoresis(CZE). The results showed that CZE can be used as asupplement method for comparing and differentiating the rice cultivars, especially some hy-brids, which are difficult to differentiate by polyacrylamide gel electrophoresis.展开更多
文摘利用A—PAGE（Acid—polyacrylamide gel electrophoresis）技术对来源于32个国家的53份红花材料进行了种子醇溶蛋白检测。结果表明，这些红花材料具有丰富的醇溶蛋白等位变异，共分离出15条迁移率不同的谱带。其中，每份材料可电泳出5-12条谱带，平均8．5条，所有材料有1条共有带。红花种子醇溶蛋白可作为评价红花遗传多样性的工具之一。不同材料的遗传相似系数（GS）变异范围为0．375—1．000，平均值为0．752。聚类分析结果表明，在GS值为0．752的水平上供试材料聚为6大类，其亲缘关系远近与地理来源关系不大。其中，来自中国的7份材料分别被聚在了3个大类中，表明中国红花种子醇溶蛋白遗传多样性比较丰富。
文摘利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。结果表明，油用型红花醇溶蛋白位点存在丰富的变异类型，共分离出15条迁移率不同的谱带，每份材料具有3—12条不等，平均8．5条。材料间平均遗传相似系数（GS）为0．5303，变幅为0．1333—1．000，且各洲间遗传多样性大于洲内遗传多样性。在GS值为0．512的水平上，供试材料聚为六大类，聚类结果表明，红花醇溶蛋白图谱类型与其地理分布有一定的相关，但不显著。
基金supported by grants from the National Natural Science Foundation of China(NSFC31871396,32000208,32070769)the Science and Technology Innovation Program of Hunan Province(2020WK2014)+2 种基金the Changsha Municipal Natural Science Foundation(kq2014039)the Scientific Innovation Fund for Post-graduates of Central South University of Forestry and Technology(CX202101026)the Postgraduate Scientific Research Innovation Project of Hunan Province(CX20210844)。
文摘Rice(Oryza sativa L.)is the most important food crop for at least half of the world’s population.Due to improved living standards,the cultivation of high-quality rice for different purposes and markets has become a major goal.Rice quality is determined by the presence of many nutritional components,including seed storage proteins(SSPs),which are the second most abundant nutrient components of rice grains after starch.Rice SSP biosynthesis requires the participation of multiple organelles and is influenced by the external environment,making it challenging to understand the molecular details of SSP biosynthesis and improve rice protein quality.In this review,we highlight the current knowledge of rice SSP biosynthesis,including a detailed description of the key molecules involved in rice SSP biosynthetic processes and the major environmental factors affecting SSP biosynthesis.The effects of these factors on SSP accumulation and their contribution to rice quality are also discussed based on recent findings.This recent knowledge suggests not only new research directions for exploring rice SSP biosynthesis but also innovative strategies for breeding high-quality rice varieties.
文摘为了考察红花的种内变异,为进一步进行红花种质资源选育奠定基础。利用A-PAGE（Acid-polyacrylamide gel electrophoresis）技术对来源于中国不同地区的19份红花材料醇溶蛋白位点进行检测。结果表明,红花种子醇溶蛋白位点存在丰富的变异类型,共分离出13条迁移率不同的谱带,每份材料具有8-11条不等,平均10条。材料间平均遗传相似系数（GS）为0.759,变幅为0.385-1.000。在GS值为0.25的水平上,供试材料聚为两大类,聚类结果表明,红花醇溶蛋白图谱类型与其地理分布有很大的相关性。
文摘Prolamins from thirteen kinds of homemade rice seed have been preliminary stu-dled by capillary zone electrophoresis(CZE). The results showed that CZE can be used as asupplement method for comparing and differentiating the rice cultivars, especially some hy-brids, which are difficult to differentiate by polyacrylamide gel electrophoresis.
