目的:探究稳定期慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)频繁急性加重的影响因素及其与血清分泌型卷曲相关蛋白1(secreted frizzled-related protein 1,SFRP1)、颗粒蛋白前体(progranulin,PGRN)水平的相关性。方...目的:探究稳定期慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)频繁急性加重的影响因素及其与血清分泌型卷曲相关蛋白1(secreted frizzled-related protein 1,SFRP1)、颗粒蛋白前体(progranulin,PGRN)水平的相关性。方法:选取2020年4月至2021年2月于汕头市大峰医院呼吸内科就诊的稳定期COPD患者为研究对象,根据患者既往12个月内症状急性加重的情况,将其分为频繁急性加重组和非频繁急性加重组。采集所有患者一般资料,进行肺功能检查、常规生化指标及血清SFRP1、PGRN水平测定,并行改良英国医学研究委员会呼吸困难量表(modified Medical Research Council Dyspnea Scale,mMRC)、临床慢性阻塞性肺病问卷(Clinical COPD Questionnaire,CCQ)、Charlson合并症指数(Charlson Comorbidity Index,CCI)、汉密尔顿焦虑量表(Hamilton Anxiety Scale,HAMA)评估。采用logistic回归模型分析相关指标与COPD频繁急性加重的关系。结果:共入组160例,其中频繁急性加重组为92例,非频繁急性加重组68例。与非频繁急性加重组相比,频繁急性加重组的体重指数(body mass index,BMI)、第1秒用力呼气容积(forced expiratory volume in one second,FEV_(1))、FEV_(1)/用力肺活量(forced vital capacity,F VC)及血清肾小球滤过率(glomerular filtration rate,GFR)、血红蛋白(haemoglobin,Hb)水平均明显更低(P<0.05),血清总免疫球蛋白E(immunoglobulin E,IgE)、SFRP1、PGRN水平及慢性阻塞性肺病评估测试(COPD assessment test,CAT)、HAMA评分则均明显更高(P<0.05)。Logistic回归分析显示:BMI[优势比(odds ratio,OR)=0.973]、FEV_(1)(OR=0.963)、GFR(OR=0.865)、血清总IgE(OR=3.473)、SFRP1(OR=2.081)、PGRN(OR=2.612)及CAT评分(OR=1.101)、HAMA评分(OR=1.134)均为稳定期COPD频繁急性加重的独立影响因素(P<0.05)。结论:相比非频繁急性加重患者,COPD频繁急性加重患者存在BMI较低、肺功能较差等特点,且血清SFRP1、PGRN水平与频繁急性加重具有明显�展开更多
In China,moxibustion is reported to be useful and has few side effects for chronic fatigue syndrome,but its mechanisms are largely unknown.More recently,the focus has been on the wealth of information supporting stres...In China,moxibustion is reported to be useful and has few side effects for chronic fatigue syndrome,but its mechanisms are largely unknown.More recently,the focus has been on the wealth of information supporting stress as a factor in chronic fatigue syndrome,and largely concerns dysregulation in the stress-related hypothalamic-pituitary-adrenal axis.In the present study,we aimed to determine the effect of moxibustion on behavioral symptoms in chronic fatigue syndrome rats and examine possible mechanisms.Rats were subjected to a combination of chronic restraint stress and forced swimming to induce chronic fatigue syndrome.The acupoints Guanyuan（CV4） and Zusanli（ST36,bilateral） were simultaneously administered moxibustion.Untreated chronic fatigue syndrome rats and normal rats were used as controls.Results from the forced swimming test,open field test,tail suspension test,real-time PCR,enzyme-linked immunosorbent assay,and western blot assay showed that moxibustion treatment decreased m RNA expression of corticotropin-releasing hormone in the hypothalamus,and adrenocorticotropic hormone and corticosterone levels in plasma,and markedly increased progranulin m RNA and protein expression in the hippocampus.These findings suggest that moxibustion may relieve the behavioral symptoms of chronic fatigue syndrome,at least in part,by modulating the hypothalamic-pituitary-adrenal axis and upregulating hippocampal progranulin.展开更多
Progranulin （PGRN） is a growth factor implicated in various pathophysiological processes, including wound healing, inflammation, tumorigenesis, and neurodegeneration. It was previously reported that PGRN binds to tu...Progranulin （PGRN） is a growth factor implicated in various pathophysiological processes, including wound healing, inflammation, tumorigenesis, and neurodegeneration. It was previously reported that PGRN binds to tumor necrosis factor receptors （TNFR） and has thera- peutic effects in inflammatory arthritis （Tang et. al, in Science 332：478-484, 2011）; however, Chen et al. reported their inability to demonstrate the PGRN-TNFR interactions under their own conditions （Chert et. al, in J Neurosci 33：9202-9213, 2013）. A letter-to-editor was then published by the original group in response to the Chen et al. paper that discussed the reasons for the latter＇s inability to recapitulate the interactions. In addition, the group published follow-up studies that further reinforced and dissected the interactions of PGRN- TNFR. Recently, the dispute about the legitimacy of PGRN-TNFR interactions appears to be finally settled with independent confirmations of these interactions in various conditions by numerous laboratories. This review presents a chronological update on the story of PGRN-TNFR interactions, highlighting the independent confirmations of these interactions in various diseases and conditions.展开更多
文摘目的:探究稳定期慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)频繁急性加重的影响因素及其与血清分泌型卷曲相关蛋白1(secreted frizzled-related protein 1,SFRP1)、颗粒蛋白前体(progranulin,PGRN)水平的相关性。方法:选取2020年4月至2021年2月于汕头市大峰医院呼吸内科就诊的稳定期COPD患者为研究对象,根据患者既往12个月内症状急性加重的情况,将其分为频繁急性加重组和非频繁急性加重组。采集所有患者一般资料,进行肺功能检查、常规生化指标及血清SFRP1、PGRN水平测定,并行改良英国医学研究委员会呼吸困难量表(modified Medical Research Council Dyspnea Scale,mMRC)、临床慢性阻塞性肺病问卷(Clinical COPD Questionnaire,CCQ)、Charlson合并症指数(Charlson Comorbidity Index,CCI)、汉密尔顿焦虑量表(Hamilton Anxiety Scale,HAMA)评估。采用logistic回归模型分析相关指标与COPD频繁急性加重的关系。结果:共入组160例,其中频繁急性加重组为92例,非频繁急性加重组68例。与非频繁急性加重组相比,频繁急性加重组的体重指数(body mass index,BMI)、第1秒用力呼气容积(forced expiratory volume in one second,FEV_(1))、FEV_(1)/用力肺活量(forced vital capacity,F VC)及血清肾小球滤过率(glomerular filtration rate,GFR)、血红蛋白(haemoglobin,Hb)水平均明显更低(P<0.05),血清总免疫球蛋白E(immunoglobulin E,IgE)、SFRP1、PGRN水平及慢性阻塞性肺病评估测试(COPD assessment test,CAT)、HAMA评分则均明显更高(P<0.05)。Logistic回归分析显示:BMI[优势比(odds ratio,OR)=0.973]、FEV_(1)(OR=0.963)、GFR(OR=0.865)、血清总IgE(OR=3.473)、SFRP1(OR=2.081)、PGRN(OR=2.612)及CAT评分(OR=1.101)、HAMA评分(OR=1.134)均为稳定期COPD频繁急性加重的独立影响因素(P<0.05)。结论:相比非频繁急性加重患者,COPD频繁急性加重患者存在BMI较低、肺功能较差等特点,且血清SFRP1、PGRN水平与频繁急性加重具有明显�
基金funded by the National Natural Science Foundation (No.81303034 and 81303031)China Postdoctoral Science Foundation (No.KLF501004)Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine
文摘In China,moxibustion is reported to be useful and has few side effects for chronic fatigue syndrome,but its mechanisms are largely unknown.More recently,the focus has been on the wealth of information supporting stress as a factor in chronic fatigue syndrome,and largely concerns dysregulation in the stress-related hypothalamic-pituitary-adrenal axis.In the present study,we aimed to determine the effect of moxibustion on behavioral symptoms in chronic fatigue syndrome rats and examine possible mechanisms.Rats were subjected to a combination of chronic restraint stress and forced swimming to induce chronic fatigue syndrome.The acupoints Guanyuan（CV4） and Zusanli（ST36,bilateral） were simultaneously administered moxibustion.Untreated chronic fatigue syndrome rats and normal rats were used as controls.Results from the forced swimming test,open field test,tail suspension test,real-time PCR,enzyme-linked immunosorbent assay,and western blot assay showed that moxibustion treatment decreased m RNA expression of corticotropin-releasing hormone in the hypothalamus,and adrenocorticotropic hormone and corticosterone levels in plasma,and markedly increased progranulin m RNA and protein expression in the hippocampus.These findings suggest that moxibustion may relieve the behavioral symptoms of chronic fatigue syndrome,at least in part,by modulating the hypothalamic-pituitary-adrenal axis and upregulating hippocampal progranulin.
文摘Progranulin （PGRN） is a growth factor implicated in various pathophysiological processes, including wound healing, inflammation, tumorigenesis, and neurodegeneration. It was previously reported that PGRN binds to tumor necrosis factor receptors （TNFR） and has thera- peutic effects in inflammatory arthritis （Tang et. al, in Science 332：478-484, 2011）; however, Chen et al. reported their inability to demonstrate the PGRN-TNFR interactions under their own conditions （Chert et. al, in J Neurosci 33：9202-9213, 2013）. A letter-to-editor was then published by the original group in response to the Chen et al. paper that discussed the reasons for the latter＇s inability to recapitulate the interactions. In addition, the group published follow-up studies that further reinforced and dissected the interactions of PGRN- TNFR. Recently, the dispute about the legitimacy of PGRN-TNFR interactions appears to be finally settled with independent confirmations of these interactions in various conditions by numerous laboratories. This review presents a chronological update on the story of PGRN-TNFR interactions, highlighting the independent confirmations of these interactions in various diseases and conditions.
